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  4. Phospho-ARHGEF2 (S885) Antibody

Phospho-ARHGEF2 (S885) Antibody

  • 货号：
    CSB-PA070250
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    Q92974
  • 基因名：
    ARHGEF2
  • 别名：
    AA408978 antibody; ARHG2 antibody; ARHG2_HUMAN antibody; ARHGEF 2 antibody; ARHGEF-2 antibody; ARHGEF2 antibody; GEF antibody; GEF H1 antibody; GEF-H1 antibody; GEFH1 antibody; Guanine nucleotide exchange factor H1 antibody; KIAA0651 antibody; Lbcl1 antibody; Lfc antibody; LFP40 antibody; MGC95068 antibody; Microtubule-regulated Rho-GEF antibody; mKIAA0651 antibody; P40 antibody; Proliferating cell nucleolar antigen p40 antibody; Protein GEF-H1 antibody; Rho guanine nucleotide exchange factor 2 antibody; rho/rac guanine nucleotide exchange factor (GEF) 2 antibody; rho/rac guanine nucleotide exchange factor 2 antibody; rho/rac guanine nucleotide exchange factor antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human Lfc around the phosphorylation site of S885.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability. Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons.
  • 基因功能参考文献：
    1. s identified a regulatory switch controlled by MARK3 that couples microtubules to the actin cytoskeleton to establish epithelial cell polarity through ARHGEF2. PMID: 29089450
    2. human brain malformation is recapitulated in Arhgef2 mutant mice and identify an aberrant migration of distinct components of the precerebellar system as a pathomechanism underlying the midbrain-hindbrain phenotype. Our results highlight the crucial function of ARHGEF2 in human brain development and identify a mutation in ARHGEF2 as novel cause of a neurodevelopmental disorder. PMID: 28453519
    3. Overexpression of miR-194 downregulates the GEF-H1/RhoA pathway, inhibits melanoma cancer cell proliferation and metastasis. Furthermore, miR-194 expression is negatively associated with tumor-node-metastasis (TNM) stages PMID: 27573550
    4. data suggest that the induction of SGK1 through treatment with dexamethasone alters MT dynamics to increase Sec5-GEF-H1 interactions, which promote GEF-H1 targeting to adhesion sites. PMID: 26359301
    5. this study reports the crystal structure of human GEF-H1 PH domain to 2.45 A resolution. PMID: 26820534
    6. By stimulating cofilin/PP2A-mediated dephosphorylation of the guanine nucleotide exchange factor GEF-H1. PMID: 26759237
    7. regulation of c-Src trafficking requires both microtubules and actin polymerization, and GEF-H1 coordinates c-Src trafficking, acting as a molecular switch between these two mechanisms PMID: 26866809
    8. Results supported that miR-512-3p could inhibit tumor cell adhesion, migration, and invasion by regulating the RAC1 activity via DOCK3 in NSCLC A549 and H1299 cell lines. PMID: 25687035
    9. TGF-beta regulates LARG and GEF-H1 during epithelial-mesenchymal transition to affect stiffening response to force and cell invasion. PMID: 25143398
    10. Our findings underscore a potent oncogenic role for GEF-H1 in promoting the metastatic potentials of hepatocellular carcinoma, possibly through activation of RhoA signalling. PMID: 22847784
    11. Paxillin-GEF-H1-p42/44-MAPK module as a regulator of pathological mechanotransduction. PMID: 24706358
    12. This study investigated a novel mechanism of vascular barrier protection by ANP via modulation of GEF-H1 function. PMID: 24352660
    13. The RhoGEF GEF-H1 is required for oncogenic RAS signaling via KSR-1. PMID: 24525234
    14. CAMSAP3-anchored non-centrosomal microtubules capture GEF-H1 more efficiently than other microtubules do. PMID: 23432781
    15. ERK binds to the Rho exchange factor GEF-H1 and phosphorylates it on S959, causing inhibition of GEF-H1 activity and a consequent decrease in RhoA activity. PMID: 24043311
    16. vincristine activates GEF-H1/RhoA/ROCK/MLC signaling. PMID: 23057787
    17. extracellular matrix stiffness regulates RhoA through microtubule destabilization and the subsequent release and activation of GEF-H1. PMID: 22593214
    18. present evidence that depletion of GEF-H1, a guanine nucleotide exchange factor for Rho proteins, affects vesicle trafficking PMID: 22898781
    19. Data indicate that highly aggressive spindle-shaped 231BR3 cells changed to a round cell morphology associated with expression of the small GTPase guanine nucleotide exchange factor-H1 (GEF-H1). PMID: 22513363
    20. Study shows that hPTTG1 is a transcription factor that triggers the GEF-H1/RhoA pathway to accelerate breast cancer invasion and metastasis. In human invasive breast carcinoma, hPTTG1 is overexpressed and is correlated to GEF-H1 expression. PMID: 22002306
    21. LPS rapidly upregulates GEF-H1 expression. Activated Rho-associated kinase by GEF-H1 subsequently activates p38 and ERK1/2, thereby increasing IL-6/TNF-alpha expression in endothelial cells. PMID: 22301607
    22. Lipopolysaccharide-induced ICAM-1 synthesis in human umbilical vein endothelial cells is regulated by GEF-H1/RhoA-dependent signaling pathway via activation of p38 and NF-kappaB. PMID: 22226621
    23. Results show that LPS-induced NF-kappaB activation and IL-8 synthesis in endothelial cells are regulated by the MyD88 pathway and GEF-H1-RhoA pathway. PMID: 22226472
    24. Polarity-regulating kinase partitioning-defective 1b (PAR1b) phosphorylates guanine nucleotide exchange factor H1 (GEF-H1) to regulate RhoA-dependent actin cytoskeletal reorganization. PMID: 22072711
    25. These data suggest that Par1b-phosphorylation regulates turnover of GEF-H1 localization by regulating its interaction with microtubules, which may contribute to cell polarization. PMID: 21513698
    26. Heparin inhibits pulmonary artery smooth muscle cell proliferation through GEF-H1/RhoA/ROCK/p27 signaling pathway. PMID: 20558775
    27. GEFH1 was identified as binding partner for the BAR domain of ASAP1.GEFH1 is a negative regulator of podosomes. PMID: 21352810
    28. the TNF-alpha-induced activation of the ERK/GEF-H1/RhoA pathway in tubular cells is mediated through Src- and TACE-dependent EGFR activation. PMID: 21212278
    29. GEF-H1 is a new component of a syndecan signaling complex that is differentially expressed in brain metastatic melanoma cells compared to corresponding non-metastatic counterparts PMID: 20803552
    30. Data indicate that GEF-H1 is a target and functional effector of TGF-beta by orchestrating Rho signaling to regulate gene expression and cell migration. PMID: 20089843
    31. GEF-H1 is regulated by an interaction with microtubules. PMID: 11912491
    32. PAK4 mediates morphological changes through regulation of GEF-H1 PMID: 15827085
    33. GEF-H1 expression level strongly correlated with p53 status in a panel of 32 cancer cell lines, and GEF-H1 induction caused activation of RhoA. Growth of mutant p53 cells was dependent on GEF-H1 expression. PMID: 16778209
    34. These results identify a GEF-H1-dependent mechanism to modulate localized RhoA activation during cytokinesis under the control of mitotic kinases. PMID: 17488622
    35. These studies reveal a critical role for a GEF-H1/RhoA/ROCK/MLC signaling pathway in mediating nocodazole-induced cell contractility. PMID: 18287519
    36. This study establishes GEF-H1 as a critical organizer of key structural and signaling components of cell migration through the localized regulation of RhoA activity at the cell leading edge. PMID: 19625450
    37. Lfc is a phosphorylated protein and demonstrate that 14-3-3 interacts directly and in a phosphorylation-dependent manner with Lfc. PMID: 19667072

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  • 相关疾病：
    Neurodevelopmental disorder with midbrain and hindbrain malformations (NEDMHM)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm. Cell junction, tight junction. Golgi apparatus. Cytoplasm, cytoskeleton, spindle. Cell projection, ruffle membrane. Cytoplasmic vesicle.
  • 数据库链接：

    HGNC: 682

    OMIM: 607560

    KEGG: hsa:9181

    STRING: 9606.ENSP00000354837

    UniGene: Hs.743352