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  4. Phospho-ARAF (Y302) Antibody

Phospho-ARAF (Y302) Antibody

  • 货号：
    CSB-PA011270
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    P10398
  • 基因名：
  • 别名：
    A raf 1 antibody; A Raf proto oncogene serine/threonine protein kinase antibody; ARAF 1 antibody; Araf antibody; ARaf proto oncogene serine/threonine protein kinase antibody; ARAF_HUMAN antibody; ARAF1 antibody; Oncogene Araf1 antibody; Oncogene PKS2 antibody; PKS 2 antibody; PKS antibody; PKS2 antibody; Proto oncogene Pks antibody; Proto-oncogene A-Raf antibody; Proto-oncogene A-Raf-1 antibody; Proto-oncogene Pks antibody; RAFA 1 antibody; RAFA1 antibody; Ras binding protein DA Raf antibody; Serine/threonine-protein kinase A-Raf antibody; v raf murine sarcoma 3611 viral oncogene homolog antibody; v raf murine sarcoma 3611 viral oncogene homolog 1 antibody; v raf oncogene homolog 1 (murine sarcoma 3611 virus) antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human A-Raf around the phosphorylation site of Y302.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.; Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
  • 基因功能参考文献：
    1. The constitutive or induced re-localization of A-Raf to the plasma membrane compromises its ability to efficiently sequester and inactivate MST2, thus rendering cells susceptible to apoptosis PMID: 26891695
    2. This review discusses the regulation of A-Raf protein expression, and the roles of A-Raf in apoptosis and cancer, with a special focus on its role in resistance to Raf inhibitors. [review] PMID: 26508523
    3. analysis of FGFR2-PPHLN1 fusion and ARAF mutations in intrahepatic cholangiocarcinoma PMID: 25608663
    4. Aberrant expression of A-, B-, and C-RAF, and COT is frequent in PTC; increased expression of COT is correlated with recurrence of PTC. PMID: 25674762
    5. Dimerization of the kinase ARAF promotes MAPK pathway activation and cell migration. PMID: 25097033
    6. The study identifies somatic activating ARAF mutations in Langerhans cell histiocytosis. PMID: 24652991
    7. Galpha12-ARAF-ERK pathway stimulates RFFL transcription through the transcription factor c-Myc. PMID: 24114843
    8. show that Araf antagonizes mesendoderm induction and patterning activity of Nodal/Smad2 signals in vertebrate embryos by directly inhibiting Smad2 signalling PMID: 23591895
    9. study investigated role of ARAF in cancer cell signaling and examined the role of ARAF in mediating paradoxical activation of the MAPK pathway in cells treated with RAF inhibitors; ARAF seems to stabilize BRAF:CRAF complexes in cells treated with RAF inhibitors and regulate cell signaling in a subtle manner to ensure signaling efficiency PMID: 22926515
    10. Ras pathway activation via EGF treatment induced strong binding between B-Raf and C-Raf and a low level of binding between B-Raf and A-Raf. PMID: 23352452
    11. hnRNP H blocks MST2-mediated apoptosis in cancer cells by regulating A-Raf transcription. PMID: 20145135
    12. In a two-hybrid screen of human fetal liver cDNA library, TH1 was detected as a new interaction partner of A-Raf; this specific interaction may have played a critical role in the activation of A-Raf. PMID: 11952167
    13. A-Raf kinase is negatively regulated by trihydrophobin 1 PMID: 14684750
    14. mutation analysis of the conserved regions in the ARAF gene in human colorectal adenocarcinoma PMID: 14688025
    15. A-Raf residues are identified that bind to specific phosphoinositides, possibly as a mechanism to localize the enzyme to particular membrane microdomains rich in these phospholipids. PMID: 15736953
    16. These data reveal that B-RAF is an important mediator of neuronal survival, migration, and dendrite formation and that A-RAF cannot fully compensate for these functions. PMID: 16980614
    17. Positive regulation of A-RAF by phosphorylation of isoform-specific hinge segment and identification of novel phosphorylation sites. PMID: 18662992
    18. A-Raf interacts with MEK1 and activates MEK1 by phosphorylation. PMID: 8621729

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  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
  • 组织特异性：
    Predominantly in urogenital tissues.
  • 数据库链接：

    HGNC: 646

    OMIM: 311010

    KEGG: hsa:369

    STRING: 9606.ENSP00000366244

    UniGene: Hs.446641