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  4. PUM2 Antibody

PUM2 Antibody

  • 货号：
    CSB-PA019082GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    Q8TB72
  • 基因名：
  • 别名：
    FLJ36528 antibody; KIAA0235 antibody; MGC138251 antibody; MGC138253 antibody; PUM 2 antibody; PUM2 antibody; PUM2_HUMAN antibody; PUMH 2 antibody; PUMH2 antibody; Pumilio (Drosphila) homolog 2 antibody; Pumilio homolog 2 (Drosophila) antibody; Pumilio homolog 2 antibody; Pumilio-2 antibody; Pumilio2 antibody; PUML2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Human PUM2
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Plays a role in cytoplasmic sensing of viral infection. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. May regulate DCUN1D3 mRNA levels. May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level.
  • 基因功能参考文献：
    1. PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3'UTR with miRNAs PMID: 30084199
    2. A recent paper by the Mendell group identifies NORAD, a novel lncRNA that is regulated in response to DNA damage and plays a key role in maintaining genome integrity by modulating the activity the RNA binding proteins PUM2 and PUM1. PMID: 27157388
    3. results reveal a novel regulatory pathway, underscoring a previously unknown and interconnected key role of PUM1/2 and FOXP1 in regulating normal hematopoietic stem/progenitor cell and leukemic cell growth. PMID: 28232582
    4. The study shows that pseudouridine and N(6)-methyladenosine weaken the binding of the human single-stranded RNA binding protein Pumilio 2 (hPUM2) to its consensus motif. PMID: 28138061
    5. Pumilios (including PUM1 and PUM2) are RNA-binding proteins with Puf domains made up of 8 poorly conserved Puf repeats, 3 helix bundles arranged in rainbow architecture, where each repeat recognizes a single base of the RNA-binding sequence. [REVIEW] PMID: 26517885
    6. Identification of NORAD-interacting proteins revealed that this lncRNA functions as a multivalent binding platform for PUM proteins, with the capacity to sequester a significant fraction of the total cellular pool of PUM1 and PUM2. Studies reveal unanticipated roles for a lncRNA and PUMILIO proteins in the maintenance of genomic stability. PMID: 26724866
    7. PUM2 does not repress differentiation of adipose-derived stem cells (ASCs) but rather is involved in the positive control of ASCs division and proliferation. PMID: 21649561
    8. human Pumilio homologs Pum 1 and Pum 2 repress the translation of E2F3 by binding to the E2F3 3' untranslated region (UTR) and also enhance the activity of multiple E2F3 targeting microRNAs (miRNAs) PMID: 22345517
    9. Mammalian PUM2-Ago-eEF1A inhibited translation of nonadenylated and polyadenylated reporter mRNAs in vitro. PMID: 22231398
    10. in mitosis, PUM2 physically associates with Aurora-A to ensure enough active Aurora-A at centrosomes for mitotic entry PMID: 21589936
    11. Three distinct modes of target RNA binding by PUM2 around the fifth mRNA base were observed. PMID: 21397187
    12. PUM2 as a component of conserved cellular machinery that may be required for germ cell development. PMID: 12511597
    13. PUM2 and DAZL, are capable of binding the same mRNA sequences in the 3'UTR of human SDAD1 mRNA. PMID: 15607425
    14. P2P-R expression may be translationally regulated by PUM2 PMID: 15617101
    15. PUMILIO mediated translational regulation may be universally used in developmental processes of the human body. PMID: 16967088
    16. Variation in the PUMILIO2 gene is very low and it seems improbable that mutations of this gene significantly contribute to male infertility in humans. PMID: 17154300
    17. Data found that PUMILIO2 interacts also with SNAPIN, a modulator of SNARE complex assembly, which is involved in vesicle trafficking. PMID: 19168546

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  • 亚细胞定位：
    Cytoplasm. Cytoplasmic granule. Cytoplasm, perinuclear region.
  • 组织特异性：
    Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach E
  • 数据库链接：

    HGNC: 14958

    OMIM: 607205

    KEGG: hsa:23369

    STRING: 9606.ENSP00000338173

    UniGene: Hs.467824