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  4. PTK6 Antibody

PTK6 Antibody

  • 中文名称：
    PTK6兔多克隆抗体
  • 货号：
    CSB-PA040089
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    Q13882
  • 基因名：
    PTK6
  • 别名：
    Breast tumor kinase antibody; Breast tumour kinase antibody; Protein tyrosine kinase 6 antibody; Protein-tyrosine kinase 6 antibody; Ptk6 antibody; PTK6_HUMAN antibody; Tyrosine protein kinase BRK antibody; Tyrosine-protein kinase BRK antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from the N-terminal region of Human Brk.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.; Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.
  • 基因功能参考文献：
    1. Using small molecule inhibitors, findings suggest that the kinase activity of PTK6 does not play a significant role in breast tumorigenesis. PMID: 29879184
    2. These results indicate that plasma-membrane-associated PTK6 phosphorylates Eps8, which promotes cell proliferation, adhesion, and migration PMID: 28214294
    3. Expression of PTK6 in established tumors may maintain the epithelial phenotype, preventing tumor progression. PMID: 26983689
    4. analysis of recombinant PTK6 kinase domain by x-ray crystallography reveals details of its binding to inhibitors PMID: 27993680
    5. The crystal structure of the kinase domain of human PTK6 has been reported. PMID: 27480927
    6. PTK6 mRNA and protein levels were elevated in cervical squamous cell cancer and the elevated cytoplasmic expression of PTK6 was associated with tumor grade and short patient survival. PMID: 27311570
    7. PELP1 interacted with GR to activate Brk expression. PMID: 26825173
    8. PTK6 prolongs S-phase and increases the ability of gemcitabine to cause DNA damage in vitro and in vivo PMID: 26013168
    9. BRK expression in a majority of CC can interact with RTK, augmenting growth and interfering with proliferation inhibitors (SAM68). Therapeutically targeting BRK function (in addition to RTK) should be of benefit for CC treatment. PMID: 25770659
    10. Additive impact of HER2-/PTK6-RNAi on interactions with HER3 or IGF-1R leads to reduced breast cancer progression PMID: 25241146
    11. Establishment of cross talk between PTK6 and CagA by functional studies may further elucidate the underlying biology of H. pylori-mediated gastric cancer PMID: 25838168
    12. PTK6 is expressed in the normal human mammary gland, but does not appear to be active and may have kinase-independent functions that are distinct from its cancer promoting activities at the membrane. PMID: 25153721
    13. Down-regulated expression of PTK6 is correlated with poor survival in esophageal squamous cell carcinoma. PMID: 25377660
    14. PTP1B inhibited BRK by dephosphorylating Tyr-342, but activated SRC by antagonizing PAG-dependent inhibition by CSK. PMID: 25897081
    15. Activating mutations in BRK in breast cancer may disrupt intramolecular interactions that normally maintain BRK in an autoinhibited conformation. PMID: 25940761
    16. This study shows that Brk phosphorylates p27KIP1, regulating the activity of cyclin D-cyclin-dependent kinase 4. PMID: 25733683
    17. Hypoxia-induced PTK6 stabilization and subcellular re-localization to the plasma membrane include increased cell motility and invasion, suggesting PTK6 targeting as a therapeutic approach to reduce hypoxia-regulated metastatic potential. PMID: 25019382
    18. PTK6 regulates cellular migration and invasion in pancreatic cancer via ERK signaling. PTK6 may be a novel therapeutic target for pancreatic cancer. PMID: 24788754
    19. BRK has a role in targeting Dok1 for ubiquitin-mediated proteasomal degradation and in promoting cell proliferation and migration PMID: 24523872
    20. Degradation of Brk involved a calpain-1-mediated proteolytic pathway and indicated an inverse relationship between the level of HER2 expression and calpain-1 activity. PMID: 23707532
    21. Protein tyrosine kinase 6 may have a role in poor prognosis and metastasis of nasopharyngeal carcinoma PMID: 23758975
    22. Highly active PTK6 promoted epithelial-mesenchymal transition by enhancing activation of AKT, thereby stimulating cancer cell migration and metastases in xenograft models of prostate cancer. PMID: 23856248
    23. High PTK6 expression is associated with hypoxia-associated breast cancer progression. PMID: 23928995
    24. an important role for PTP1B as a negative regulator of BRK and IGF-1Rbeta signaling in ovarian cancer cells. PMID: 23814047
    25. Protein tyrosine kinase 6 protects cells from anoikis by directly phosphorylating focal adhesion kinase and activating AKT. PMID: 23027128
    26. Brk/PTK6 cooperates with HER2 and Src in regulating breast cancer cell survival and epithelial-to-mesenchymal transition. PMID: 23291984
    27. present findings indicated that cytoplasmic PTK6 expression is a potential prognostic factor for survival in LSCC patients. High expression of PTK6 was associated with favorable OS and DFS in LSCC patients PMID: 23497344
    28. These results provide evidence for a novel mechanism demonstrating the oncogenic potential of PTK6 through degradation of c-Cbl, which is an E3 ligase important in down-regulation of oncoproteins. PMID: 23352614
    29. Knockdown of PTK6 enhances apoptosis in HCT116 cells with wild-type p53. PMID: 22989419
    30. breast tumor kinase (Brk)/protein-tyrosine kinase 6 (PTK6), a nonreceptor protein-tyrosine kinase highly expressed in most human breast tumors, interacted with EGFR and sustained ligand-induced EGFR signaling. PMID: 22231447
    31. Brk expression in human breast tumors may contribute to progression by inducing p38-driven pro-survival signaling pathways. PMID: 21923922
    32. results demonstrate that Hsp90 plays an essential role in regulating PTK6 stability and suggest that Hsp90 inhibitors may be useful as therapeutic drugs for PTK6-positive cancers, including breast cancer PMID: 22849407
    33. PTK6 was identified as a transcript that is down-regulated in human esophageal squamous cell carcinoma via epigenetic modification at the PTK6 locus. PMID: 22705009
    34. Suppressor of cytokine signaling 3 inhibits breast tumor kinase activation of STAT3. PMID: 22547065
    35. a novel function for PTK6 at the plasma membrane PMID: 22084245
    36. show that as primary human keratinocytes are induced to differentiate in vitro, Brk levels decrease PMID: 21240512
    37. present findings indicate an increase of cytoplasmic Brk expression in non-small cell lung cancer PMID: 21603980
    38. Data suggest that since PTK6 plays an important role in HER receptor signal transduction, its down-regulation might be suitable for future therapy approaches in breast cancer. PMID: 21380407
    39. Alternative transcript-PTK6 is able to negatively regulate growth and modulate PTK6 activity, protein-protein associations and/or subcellular localization. PMID: 21479203
    40. Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells. PMID: 20929863
    41. Brk antagonizes the transcriptional activity of the transcription factor FoxO family of proteins by inhibiting its nuclear localization. As a result, the cell cycle inhibitor p27, a FoxO target gene, is down-regulated. PMID: 20162673
    42. PTK6 enhances EGFR signaling by inhibition of EGFR down-regulation through phosphorylation of ARAP1 in breast cancer cells. PMID: 20554524
    43. AKT is a direct substrate of PTK6 and AKT tyrosine residues 315 and 326 are phosphorylated by PTK6. PMID: 20606012
    44. PTK6 associates with nuclear and cytoplasmic beta-catenin and inhibits beta-catenin- and T-cell factor (TCF)-mediated transcription. PMID: 20026641
    45. the effect of Sam68 was inhibited in a dose-dependent manner by coexpression of an activated form of the nuclear kinase Sik/BRK that hyperphosphorylated Sam68 PMID: 12482964
    46. The tyrosine kinases Brk/PTK6/Sik, Srm, Frk/Rak/Gtk/Iyk/Bsk, and Src42A/Dsrc41 have a low degree of sequence homology to other known kinases. The exon structure of these kinases, called the Brk family, is highly conserved and distinct. PMID: 12725532
    47. BRK plays a role in differentiation of prostate epithelial cells PMID: 12833144
    48. Study suggests that SH3 interactions will govern phosphorylation of many substrates by BRK. PMID: 14676834
    49. Results describe the solution structure and backbone dynamics of the human protein-tyrosine kinase-6 Src homology 2 (SH2) domain using multidimensional NMR spectroscopy. PMID: 15056653
    50. in breast cancer cells breast tumor kinase (Brk) behaves similarly to a constitutively active Brk mutant (YF-Brk) and associates with tyrosine-phosphorylated proteins in deregulated signaling complexes PMID: 15539407

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell projection, ruffle. Membrane. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. Nuclear localization in epithelial cells of normal prostate but cytoplasmic localization in cancer prostate.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, BRK/PTK6/SIK subfamily
  • 组织特异性：
    Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Not expressed in breast or ovarian tissue but expressed in high percentage of breast and ovarian cancers. Also overexpresse
  • 数据库链接：

    HGNC: 9617

    OMIM: 602004

    KEGG: hsa:5753

    STRING: 9606.ENSP00000217185

    UniGene: Hs.51133