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PRPF19 Antibody

  • 货号：
    CSB-PA003855
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of Jurkat cells using PRP19 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q9UMS4
  • 基因名：
  • 别名：
    hPso4 antibody; NMP200 antibody; Nuclear matrix protein 200 antibody; Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody; pre mRNA processing factor 19 antibody; Pre-mRNA-processing factor 19 antibody; PRP19 antibody; PRP19/PSO4 homolog antibody; PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody; PRP19_HUMAN antibody; PRPF19 antibody; PSO4 antibody; psoralen 4 antibody; Senescence evasion factor antibody; SNEV antibody; UBOX4 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human PRP19.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:40000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome.
  • 基因功能参考文献：
    1. This work implies that the gain of Prp19 is a critical event during the progression of hepatocellular carcinoma PMID: 26959880
    2. These results demonstrate that SNEV regulates adipogenesis in hASCs and indicate that DDR capacity in general might be a pre-requisite for this process. PMID: 28041875
    3. the interactome of influenza A virus NS1 in host cells was comprehensively profiled, and our findings reveal a novel regulatory role for PRP19 in viral replication PMID: 27096427
    4. Crystal structure revealed features of the WD40 domain of human PRPF19. PMID: 28962858
    5. The results suggest that RPA phosphorylation enhances the recruitment of PRP19 to RPA-ssDNA and stimulates RPA ubiquitylation through a process requiring both PRP19 and RFWD3, thereby triggering a phosphorylation-ubiquitylation circuitry that promotes ATR activation and homologous recombination. PMID: 28666352
    6. role of Pso4 in DNA repair and DNA damage PMID: 26364595
    7. Study demonstrates that the levels of Prp19 and Cdc5L are overexpressed in hepatocellular carcinoma (HCC). Prp19 binds with Cdc5L and its downregulation results in reduction of Cdc5L. Mechanistic insights reveal that silencing Prp19 compromises translation activity of Cdc5L and facilitates lysosome-induced degradation of Cdc5L in HCC cells. PMID: 28387715
    8. We report that PRP19 expression is elevated in lung carcinoma tissues compared to non-tumor tissues PMID: 24731397
    9. These findings suggest that SKAP promotes ultraviolet rays-induced cell apoptosis by negatively regulating the anti-apoptotic protein Prp19. PMID: 24718257
    10. involvement of hPso4 in the recombinational repair of DSBs PMID: 24675077
    11. DNA damage induces down-regulation of Prp19 via impairing Prp19 stability in hepatocellular carcinoma cells. PMID: 24587161
    12. The Prp19-CDC5L complex plays a crucial role during human pre-mRNA splicing by catalytic activation of the spliceosome. PMID: 24598747
    13. Prp19 complex as the first spliceosome subcomplex that directly contributes to mitosis in vertebrates independently of its function in interphase. PMID: 24069358
    14. PRP19 directly binds RPA and localizes to DNA damage sites via RPA, promoting RPA ubiquitylation in a DNA-damage-induced manner. PMID: 24332808
    15. New insights into pre-mRNA processing factor 19 PMID: 22967061
    16. PRP19 over-expression is associated with hepatocellular carcinoma recurrence. PMID: 23220010
    17. SNEV phosphorylation at S149 is essential for mediating the cytoprotective effect of SNEV upon DNA damage/oxidative stress and partially contributes to the life span extension caused by SNEV overexpression. PMID: 22529335
    18. hPso4, once it forms a complex with Metnase, negatively regulates Metnase's TIR binding activity PMID: 20416268
    19. Purified hPrp19/CDC5L complexes exhibit an elongated, asymmetric shape with a maximum dimension of approximately 20 nm. PMID: 20176811
    20. concluded that hPso4 plays a major and previously undefined role in mammalian DNA DSB repair[Pso4] PMID: 12960389
    21. required prior to catalytic step 1 of splicing, but not for stable integration of U4/U6.U5 tri-snRNPs PMID: 15175653
    22. report the identification of two additional factors required in the DNA interstrand cross-links repair process, a previously characterized pre-mRNA splicing complex composed of Pso4/Prp19 and Werner syndrome protein(WRN) PMID: 16223718
    23. SNEV, the human orthologue of yeast PRP19, functions in splicing and homo-oligomerization of SNEV in HeLa nuclear extract is essential for spliceosome assembly. PMID: 16332694
    24. The high levels of SNEV might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells. PMID: 16388800
    25. ubiquitylated hPrp19 fails to interact with either Cdc5L or Plrg1 indicating that DNA damage can induce profound alterations to hPrp19 core complex. PMID: 17276391
    26. hPso4 is necessary to bring Metnase to the DSB sites for its function(s) in DNA repair PMID: 18263876
    27. Blom7alpha is a novel splicing factor of the K homology domain family that might be implicated in alternative splicing by helping to position the CDC5L-SNEV(Prp19-Pso4) complex at the splice sites [Blom7alpha] PMID: 19641227

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Lipid droplet.
  • 蛋白家族：
    WD repeat PRP19 family
  • 组织特异性：
    Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
  • 数据库链接：

    HGNC: 17896

    OMIM: 608330

    KEGG: hsa:27339

    STRING: 9606.ENSP00000227524

    UniGene: Hs.502705