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PRKAG3 Antibody

  • 货号：
    CSB-PA883379ESR1HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: 5-AMP-activated protein kinase subunit gamma-3 antibody at 5μg/ml
      Lane 1: Hela whole cell lysate
      Lane 2: HepG2 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 55, 52 kDa
      Observed band size: 55 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PRKAG3 Polyclonal antibody
  • Uniprot No.：
    Q9UGI9
  • 基因名：
  • 别名：
    5 AMP activated protein kinase subunit gamma 3 antibody; 5''-AMP-activated protein kinase subunit gamma-3 antibody; AAKG3_HUMAN antibody; AMPK gamma 3 chain antibody; AMPK gamma3 antibody; AMPK subunit gamma-3 antibody; AMPKG3 antibody; PRKAG3 antibody; Protein kinase AMP activated gamma 3 non catalytic subunit antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human 5\'-AMP-activated protein kinase subunit gamma-3 protein (1-210AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. The AMPK gamma3 subunit is a non-catalytic subunit with a regulatory role in muscle energy metabolism. It mediates binding to AMP, ADP and ATP, leading to AMPK activation or inhibition: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
  • 基因功能参考文献：
    1. PRKAG3-230 may be associated with sporadic Wolff-Parkinson-White (WPW) syndrome in a Taiwanese population. PMID: 27866917
    2. The mTOR pathway was significantly associated with overall and estrogen receptor-negative (ER-) breast cancer risk (P = 0.003 and 0.03, respectively). PRKAG3 (Padj = 0.0018) and RPS6KA3 (Padj = 0.061) were the leading genes for the associations with overall breast cancer risk and ER- breast cancer risk, respectively. PMID: 28582508
    3. Data suggest different gamma-isoforms in AMPK can have different effects on enzyme activation; here, activation of AMPK by compound 991 is greater if AMPK contains PRKAG2 versus PRKAG1 or PRKAG3. PMID: 28302767
    4. Low PRKAA2 expression is associated with follicular lymphoma. PMID: 23396962
    5. Through the enhancement of skeletal muscle glucose uptake and increased mitochondrial content, the R225W mutation of the gene encoding AMP-activated protein kinase (AMPK)gamma(3)may significantly enhance exercise performance. PMID: 20473479
    6. Purification and characterization of truncated human AMPK alpha 2 beta 2 gamma 3 heterotrimer from baculovirus-infected insect cells PMID: 19836452
    7. identified for the first time a mutation in the skeletal muscle-specific regulatory gamma(3) subunit of AMPK in humans PMID: 17878938
    8. the observation that the AMPKgamma3 isoform is expressed primarily in white skeletal muscle, in which it is the predominant gamma-isoform, strongly suggests that gamma3 has a key role in this tissue PMID: 14559719
    9. role of the AMPKgamma3 isoform in exercise-induced metabolic and gene regulatory responses in skeletal muscle PMID: 16306365
    10. data show that different mutations in gamma3 exert different effects on allosteric regulation of alpha2beta2gamma3 complex by AMP, whereas we find no evidence for their role in regulating the level of phosphorylation of alpha2 by upstream kinases. PMID: 17518971
    11. Genetic variability in PRKAG3 does not seem to have a major effect on glucose metabolism, but play a role in lipoprotein metabolism in humans. PMID: 17701023
    12. Variants in genes for AMPKalpha2 and AMPKgamma3 were not associated with PCOS or its component traits. PMID: 19574280
    13. Functional analysis of the pig counterpart. PMID: 10818001

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  • 蛋白家族：
    5'-AMP-activated protein kinase gamma subunit family
  • 组织特异性：
    Skeletal muscle, with weak expression in heart and pancreas.
  • 数据库链接：

    HGNC: 9387

    OMIM: 604976

    KEGG: hsa:53632

    STRING: 9606.ENSP00000233944

    UniGene: Hs.591634