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PPP1R13L Antibody

  • 货号：
    CSB-PA851965OA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA851965OA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA851965OA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PPP1R13L Polyclonal antibody
  • Uniprot No.：
    Q8WUF5
  • 基因名：
    PPP1R13L
  • 别名：
    PPP1R13L antibody; IASPP antibody; NKIP1 antibody; PPP1R13BL antibody; RAI antibody; RelA-associated inhibitor antibody; Inhibitor of ASPP protein antibody; Protein iASPP antibody; NFkB-interacting protein 1 antibody; PPP1R13B-like protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Peptide sequence from Human RelA-associated inhibitor protein (83-102AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PPP1R13L Antibody (CSB-PA851965OA01HU)，的标记方式是Non-conjugated。对于PPP1R13L Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA851965OB01HU PPP1R13L Antibody, HRP conjugated ELISA
    FITC CSB-PA851965OC01HU PPP1R13L Antibody, FITC conjugated
    Biotin CSB-PA851965OD01HU PPP1R13L Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.
  • 基因功能参考文献：
    1. Haplotypes consisting of PPP1R13L rs1970764 and ATM rs11212592 may be associated with lung cancer. Haplotypes consisting of PPP1R13L, CD3EAP and GLTSCR1 SNPs on Chr19q13.3 may associate with lung cancer risk in the Chinese population. PMID: 30128886
    2. The expression of CD3EAP exon 1 was demonstrated to be significantly associated with PPP1R13L exon 1, while CD3EAP exon 3 was significantly associated with ERCC1 exon 11 in normal and non-small cell lung cancer (NSCLC) tissues. It was observed that short transcripts of ERCC1, CD3EAP and PPP1R13L are co-expressed in A549 NSCLC cell line. PMID: 29620255
    3. Increased expression of miR-150 suppressed viability, proliferation, migration and invasion of SW480 cells. Furthermore, iASPP was a direct target of miR-150 and played a key role in its anti-colorectal cancer (CRC)function. miR-150 may be a promising predictor of prognosis in CRC patients. PMID: 29750311
    4. IASPP knockdown suppressed cell viability and DNA synthesis capacity; the effect of miR-340 inhibition was partially attenuated by iASPP inhibition. PMID: 29982095
    5. The expression of iASPP was higher in highgrade astrocytic gliomas compared with lowgrade astrocytic gliomas. PMID: 29257240
    6. Data showed that iASPP could promote tumor growth by increasing autophagic flux, and iASPP could serve as a poor prognostic factor and a potential therapeutic target in lung cancer. PMID: 29072696
    7. Sertad1 could antagonize iASPP function by hindering its entrance into nuclei to interact with P53 in leukemic cells when iASPP was in the stage of overproduction. PMID: 29179704
    8. The interactive modulation among miR-124 and iASPP in p53-mutant or -deleted cells may serve as a crucial pathway, which mediates therapy resistance when p53 is mutated or deleted, in the process of photodynamic therapy treatment of Colorectal cancer. PMID: 29022915
    9. These findings indicated that XIST may regulate the tumor growth and metastasis via miR-140-dependent iASPP regulation. Taken together, our data indicated that XIST may be an oncogenic lncRNA that promotes the proliferation and metastasis of lung cancer through the regulation of miR-140 and could be regarded as a therapeutic target in human lung cancer. PMID: 28656261
    10. FHL2 and iASPP interacted with each other and co-localized in both nucleus and cytoplasm. Either FHL2 or iASPP silenced could reduce cell proliferation, induce cell cycle arrest at G0/G1 phase, and increase cell apoptosis. PMID: 28402264
    11. the restoration of miR-124 reduces iASPP expression and leads to p53-dependent tumor suppression, suggesting a therapeutic strategy to treat iASPP-associated cervical cancer. PMID: 27765948
    12. miR-124 regulates p63 via iASPP, while p63 targets miR-155 via the modulation of STAT1 expression in colorectal cancer. PMID: 28418858
    13. TP73-AS1 inhibited the brain glioma growth and metastasis as a competing endogenous RNA (ceRNA) through miR-124-dependent iASPP regulation. PMID: 29412778
    14. findings revealed the detailed role of the miR-184/iASPP axis in Central nervous system lymphoma (CNSL) and this axis might modulate the proliferation and invasion of CNSL via regulating the PI3K/Akt signaling pathway. PMID: 28012196
    15. Data suggest that Keap1, rather than Nrf2, is critical for the recruitment of iASPP into the Keap1-Nrf2 complex PMID: 29033244
    16. Three htSNPs (haplotype-tagging single nucleotide polymorphism) (rs7354, rs14384, and rs3783501) covering 95% of the common haplotype diversity in 19p13.3-GADD45B and interaction of 19p13.3-GADD45B and 19q13.3-PPP1R13L and 19q13.3-CD3EAP variants and smoking-duration were explored in lung cancer risk Chinese. PMID: 28870783
    17. We describe the identification of a maternally inherited frameshift mutation in RAI1, causative for SMS. This is the first report about transmission of SMS from an affected parent to an offspring. PMID: 27683195
    18. These results determined PPP1R13L as the gene underlying a novel autosomal-recessive cardio-cutaneous syndrome in humans and strongly suggest that the fatal dilated cardiomyopathy during infancy is a consequence of failure to regulate transcriptional pathways necessary for tuning cardiac threshold response to common inflammatory stressors. PMID: 28069640
    19. UCA1 might promote proliferation and migration of glioma, to regulate the tumor growth and metastasis via miR-182 dependent iASPP regulation. PMID: 28137422
    20. Results demonstrate that iASPP is overexpressed in bladder cancer and promotes the malignancy of bladder cancer PMID: 28489738
    21. lncRNA H19 interacts with miR-140 to modulate glioma growth by targeting iASPP PMID: 27693036
    22. Increased expression of p53 and ASPP1 and downregulation of iASPP. PMID: 27177208
    23. Our study provides the first evidence that high iASPP-SV expression may be a novel prognostic factor and therapeutic target for glioma PMID: 26628298
    24. we were able to reproduce previously found associations between PPP1R13L and CD3EAP polymorphisms and lung cancer risk in an increased study group, and we found interactions between NFKB1 rs28362491-PPP1R13L rs1970764 and smoking duration and between CD3EAP rs735482 and smoking duration PMID: 26563375
    25. inhibitor of apoptosis-stimulating protein of p53 (iASPP) was identified to be a direct target of miR-140 in pancreatic duct adenocarcinoma specimens and cell lines PMID: 26787707
    26. we show that iASPP is a novel substrate of caspases in response to apoptotic stimuli PMID: 26646590
    27. one novel region within PPP1R13L is hypomethylated in all transient neonatal diabetes type 1 patients included in this study PMID: 27075368
    28. destabilization of p300/CBP by downregulation of iASPP expression levels appears to represent a molecular mechanism that contributes to chemoresistance in melanoma cells. PMID: 25675294
    29. One of the proteins identified, iASPP, showed reduced levels in the presence of GSK-3. Further, blocking iASPP activity increased cell death, particularly in p53 wild-type BC3 PEL cells. PMID: 26109723
    30. The present study showed that iASPP is overexpressed in oral cavity squamous cell carcinomas (OSCC)tissues and increased cytoplasmic iASPP is correlated with recurrence and poor survival outcomes in OSCC patients PMID: 25149434
    31. results suggest that NFKB1 common variants and smoking duration and smoking duration-PPP1R13L rs1970764 interaction could be concerned with the lung cancer development in a Chinese population PMID: 25917613
    32. iASPP expression may act as a predictive marker of prostate cancer progression. PMID: 25341046
    33. data highlight the importance of 14-3-3 proteins in antiviral responses and identify RelA-associated inhibitor and sirtuin 1 as novel regulators of antiviral innate immune responses. PMID: 24997996
    34. These data demonstrate that by interacting with desmoplakin and desmin, iASPP is an important regulator of desmosomal function both in vitro and in vivo. PMID: 25691752
    35. s have identified a novel mechanism modulating autophagy in keratinocytes that relies upon iASPP expression specifically reducing the interaction of Atg5-Atg12 with Atg16L1. PMID: 24777476
    36. MIRN124 binds to the 3'UTR of iASPP and suppressing mRNA expression and the proliferation of prostate tumor cells. PMID: 24966937
    37. Hematopoietic cells can be protected against apoptosis by iASPP. PMID: 24668753
    38. rs6966 (3' UTR of PPP1R13L, chr 19q13.32, P = 4.55 x 10(-9)) and rs414580 (intron 2 of MSR1, chr 8p22, P = 6.09 x 10(-8)) were significantly associated with ALL. PMID: 24604828
    39. Haplotype PPP1R13L rs4803817 polymorphism is associated with lung cancer risk. PMID: 24140460
    40. overexpression of iASPP and the low expression of caspase-9 in esophageal cancer are closely correlated with tumor invasion and metastasis PMID: 24405603
    41. higher rate Helicobacter pylori infection, an increased expression of inhibitor of apoptosis stimulating protein of p53 (iASPP), and decreased expression of apoptosis-stimulating of p53 protein 2(ASPP2) was present in gastric cancer PMID: 23528480
    42. This study thus demonstrates that iASPP is highly elevated in human cervical cancer, and that overexpression of nuclear iASPP is correlated with poor prognosis and chemoresistance/radioresistance PMID: 23420450
    43. Downregulation of miR-124 promotes the growth and invasiveness of glioblastoma cells involving upregulation of PPP1R13L. PMID: 23624869
    44. the miR-124/iASPP axis can regulate the proliferation of colorectal cancer cells. PMID: 23691514
    45. PPP1R13L and CD3EAP variants may be associated with lung cancer risk in nonsmoking Chinese women. PMID: 23624123
    46. results suggest iASPP may contribute to the malignant progression of head and neck squamous cell carcinoma PMID: 22815155
    47. The PPP1R13L rs1970764 variant is a possible prognostic marker for patients with rectal cancer. PMID: 23180017
    48. When the Px(T)PxR motif is deleted or mutated via insertion of a phosphorylation site mimic (T311D), PP-1c fails to bind to all three ASPP proteins, ASPP1, ASPP2 and iASPP. PMID: 23088536
    49. These findings showed that iAPSS/iASPPsv reduced the growth inhibition and apoptosis induced by Dex or VP-16, with DNA damage accumulating which might promote the pathogenesis and/or progression of cancer. PMID: 22766503
    50. iASPP inhibited apoptosis independently of p53 in tumor cells, mainly by inhibiting the transcriptional activity of p63/p73 on the promoters of proapoptotic genes PMID: 22538442

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    ASPP family
  • 组织特异性：
    Highly expressed in heart, placenta and prostate. Weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte.
  • 数据库链接：

    HGNC: 18838

    OMIM: 607463

    KEGG: hsa:10848

    STRING: 9606.ENSP00000354218

    UniGene: Hs.466937