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POLR1A Antibody

  • 中文名称：
    POLR1A兔多克隆抗体
  • 货号：
    CSB-PA018322GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    O95602
  • 基因名：
    POLR1A
  • 别名：
    A190 antibody; AFDCIN antibody; DNA-directed RNA polymerase I largest subunit antibody; DNA-directed RNA polymerase I subunit A antibody; DNA-directed RNA polymerase I subunit rpa1 antibody; Polr1a antibody; Polymerase (RNA) I polypeptide A 194kDa antibody; RNA polymerase I 194 kDa subunit antibody; RNA polymerase I subunit A antibody; RNA polymerase I subunit A1 antibody; RPA1 antibody; RPA1_HUMAN antibody; RPA194 antibody; RPO1 4 antibody; RPO14 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Human POLR1A
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
  • 基因功能参考文献：
    1. Data show that BRCA1 interacts directly with the basal RNA polymerase I (Pol-I) transcription factors as well as interacting with RNA Pol-I itself. PMID: 27589844
    2. Homozygous c.2801C>T (p.(Ser934Leu)) in POLR1A was identified in two brothers with unusual neurological disease that manifested with ataxia, psychomotor retardation, cerebellar and cerebral atrophy, and leukodystrophy. PMID: 28051070
    3. Consistent with this observation, we discovered that polr1a mutant zebrafish exhibited cranioskeletal anomalies mimicking the human phenotype. polr1a loss of function led to perturbed ribosome biogenesis and p53-dependent cell death PMID: 25913037
    4. SIRT7 knockdown leads to reduced levels of RNA Pol I protein, but not messenger RNA PMID: 22586326
    5. findings demonstrate that Bloom's syndrome helicase (BLM)interacts with RPA194; studies suggest that nucleolar BLM modulates rDNA structures in association with RNA polymerase I to facilitate RNA polymerase I-mediated rRNA transcription PMID: 22106380
    6. Interference of POLR1A inhibited the synthesis of rRNA and hindered cell cycle progression in cells with inactivated p53, as a consequence of downregulation of the transcription factor E2F-1. PMID: 21878508
    7. analyzed the kinetics of assembly and elongation of the RNA polymerase I complex on endogenous ribosomal genes in the nuclei of living cells with the use of in vivo microscopy [RPA43] PMID: 12446911

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  • 相关疾病：
    Acrofacial dysostosis, Cincinnati type (AFDCIN)
  • 亚细胞定位：
    Nucleus, nucleolus. Chromosome.
  • 蛋白家族：
    RNA polymerase beta' chain family
  • 数据库链接：

    HGNC: 17264

    OMIM: 616404

    KEGG: hsa:25885

    STRING: 9606.ENSP00000263857

    UniGene: Hs.531818