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POLA1 Antibody

  • 货号：
    CSB-PA156369
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from Jurkat cells, 293 cells and HepG2 cells, using DNA Polymerase α antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) POLA1 Polyclonal antibody
  • Uniprot No.：
    P09884
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from N-terminal of Human DNA Polymerase α.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses.
  • 基因功能参考文献：
    1. High POLA1 expression is associated with bladder cancer. PMID: 28320388
    2. Divalent ions attenuate DNA synthesis by human DNA polymerase alpha by changing the structure of the template/primer or by perturbing the polymerase reaction. PMID: 27235627
    3. We propose that completely skewed XCI favoring the normal X chromosome resulted from death of cells with an active derivative X that was caused by a non-functional POLA1 gene. In summary, we conclude that functional monosomy of 6q27-qter and functional disomy of Xpter-p22.11 are responsible for the clinical phenotype of the patient PMID: 28371302
    4. The first crystal structure of human Polalpha polymerase subunit in complex with a DNA:DNA helix shows that portion of the DNA:DNA helix in contact with the polymerase is not in a B-form but in a hybrid A-B form. The free energy cost of distorting DNA from B- to hybrid A-B form may augur the termination of primer synthesis. PMID: 27032819
    5. Data indicate that X-linked reticulate pigmentary disorder (XLPDR) is caused by an intronic mutation that disrupts the expression of POLA1, which encodes the catalytic subunit of DNA polymerase-alpha. PMID: 27019227
    6. Mutation in POLA1 is responsible for XLPDR (MIM: 312040) and demonstrates a role for this gene in interferon regulation PMID: 27019227
    7. Inhibition of DNA polymerases a, delra and e by AFP promoter-driven artificial microRNAs may lead to effective growth arrest of AFP-positive HCC cells,as novel strategy for gene therapy PMID: 25924900
    8. To understand the regulatory mechanisms and to reveal the details of DNA polymerase alpha organization, the study determined the crystal structure of p70 in complex with C terminus of the POLA catalytic subunit (p180C). PMID: 25847248
    9. the N-terminal domain of the large subunit of primase (p58N) directly interacts with the C-terminal domain of the catalytic subunit of polalpha (p180C) PMID: 24962573
    10. The Pol alpha-primase complex. PMID: 22918585
    11. Pol epsilon and Pol alpha/delta seem to pursue their functions at least in part independently in late S phase PMID: 22887995
    12. Findings indicate that tethering of primase to the replisome by DNA polymerase alpha (pol alpha) is critical for the normal action of DNA replication forks in eukaryotic cells. PMID: 22593576
    13. Depletion of p180 in U2OS cells increases cell size. PMID: 22679391
    14. Human cell DNA replication is mediated by a discrete multiprotein complex. The peak of DNA polymerase alpha activity co-purifies with the peak of in vitro SV40 DNA replication activity. PMID: 11968016
    15. correlation between binding of CDP/Cux to the DNA pol alpha promoter and the stimulation of gene expression PMID: 12665598
    16. Data show that human DNA polymerase alpha and the Klenow fragment of Escherichia coli DNA polymerase I (KF) incorporate all four nucleotide analogues opposite all four canonical bases up to 4000-fold more efficiently than incorrect natural bases. PMID: 12950174
    17. Three-dimensional structures of the zinc finger motif in the carboxy terminus of the human DNA polymerase-alpha were determined in this study. PMID: 14499601
    18. nonphosphorylated p68 inhibited the stimulation of pol-alpha activity by hyperphosphorylated retinoblastoma protein, suggesting that p68 might impede the association of ppRb with p180 PMID: 16935576
    19. During dNTP polymerization, it uses a combination of negative (N-1 and N-3) and positive (N-1 and N-6) selectivity to differentiate between right and wrong dNTPs, while the shape of the base pair is essentially irrelevant. PMID: 17209555
    20. These results argue that cells can tolerate low levels of p180 as long as Mcm10 is present to "recycle" it. PMID: 17699597
    21. And-1/Ctf4 is therefore a new replication initiation factor that brings together the MCM2-7 helicase and the DNA pol alpha-primase complex. PMID: 17761813
    22. Results show that depletion of DNA polymerase alpha and not Polepsilon or Poldelta by siRNA induces phosphorylation of Chk1 on Ser345, thus phenocopying antimetabolite exposure. PMID: 19177015
    23. DNA and p180 binding to an Mcm10 construct that contains both the ID and CTD, provide the first mechanistic insight into how Mcm10 might use a handoff mechanism to load and stabilize pol alpha within the replication fork. PMID: 19608746

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  • 相关疾病：
    Pigmentary disorder, reticulate, with systemic manifestations, X-linked (PDR)
  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol.
  • 蛋白家族：
    DNA polymerase type-B family
  • 数据库链接：

    HGNC: 9173

    OMIM: 301220

    KEGG: hsa:5422

    STRING: 9606.ENSP00000368349

    UniGene: Hs.567319