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PLCB2 Antibody

  • 货号：
    CSB-PA030070HA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA030070HA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cells with CSB-PA030070HA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PLCB2 Polyclonal antibody
  • Uniprot No.：
    Q00722
  • 基因名：
  • 别名：
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC 3.1.4.11) (Phosphoinositide phospholipase C-beta-2) (Phospholipase C-beta-2) (PLC-beta-2), PLCB2
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 protein (1031-1185aa)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PLCB2 Antibody (CSB-PA030070HA01HU)，的标记方式是Non-conjugated。对于PLCB2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA030070HB01HU PLCB2 Antibody, HRP conjugated ELISA
    FITC CSB-PA030070HC01HU PLCB2 Antibody, FITC conjugated
    Biotin CSB-PA030070HD01HU PLCB2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:100-1:500
    IF 1:100-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • 基因功能参考文献：
    1. this work adds substantial information about the role of PLC-beta2 in invasive breast tumors, demonstrating that its up-regulation in cells with a basal-B triple-negative phenotype is sufficient to down-modulate the expression of surface antigens crucial for malignancy and to reduce the number of cells with a stem-like phenotype. PMID: 28870198
    2. Hypoxia modulates the expression of PLC-beta-2 in breast tumor cells in a phenotype-related manner, since a decrease of the protein was observed in the BT-474 and MCF7 cell lines while an increase was revealed in MDA-MB-231 cells as a consequence of low oxygen availability. PMID: 26785288
    3. Gnb isoforms control a signaling pathway comprising Rac1, Plcbeta2, and Plcbeta3 leading to LFA-1 activation and neutrophil arrest in vivo PMID: 26468229
    4. Neuropeptide Y decreased PLCB2 expression in HUVECs. PMID: 24903829
    5. high expression of CD133 in triple-negative breast cancer (TNBC)-derived cells correlates with high invasive potential; aggressive properties of CD133high cells are mitigated by PLC-beta2 which down-modulates expression of CD133 and may play a role in preventing metastatic progression of CD133 positive TNBC PMID: 24330829
    6. WDR26 functions as a scaffolding protein to promote PLCbeta2 membrane translocation and interaction with Gbetagamma, thereby enhancing PLCbeta2 activation in leukocytes. PMID: 23625927
    7. In this study, we show that staphylococcal enterotoxin B activates a Galphaq and PLCbeta2-dependent pathway in human T cells PMID: 23690479
    8. PLCbeta2 is enriched in the cytosol. PMID: 23006664
    9. gamma-synuclein has a role in promoting a more robust G protein Galphaq activation of PLCbeta2 PMID: 22905097
    10. GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4. PMID: 22728827
    11. WDR36 acts as a scaffold protein tethering a G-protein-coupled receptor, Galphaq and phospholipase C beta 2 in a signalling complex PMID: 21940795
    12. Rac1 activates phospholipase Cdelta1 through phospholipase Cbeta2 PMID: 20530480
    13. Data show that activation of PLCbeta(2) by alpha(q) and beta1gamma2 differ from activation by Rac2 and from each other. PMID: 20007712
    14. up-regulated during granulocytic differenetiation of normal and leukemic hematopoietic progenitors PMID: 12050180
    15. interacts with mitogen-activated protein kinase kinase 3. PMID: 12054652
    16. Conformation determines signal transduction of PLCB2 by Gbeta PMID: 12388553
    17. the recognition site for Rac GTPases was localized to the pleckstrin homology (PH) domain of PLC-beta2, and this PH domain is fully sufficient to selectively interact with the active versions of the Rac GTPases, but not with other similar Rho GTPases. PMID: 12657629
    18. Pleckstrin homology domains of phospholipases C-beta and -delta confer activation through a common site PMID: 12761218
    19. results support a model in which PLCbeta2 suppresses the basal catalytic activity of PLCdelta1, which is relieved by binding of Gbetagamma subunits to PLCbeta2 allowing for amplified calcium signals PMID: 15509571
    20. there are two modes of PLC binding to Gbetagamma through the catalytic domain, where interactions with the amino-terminal coiled-coil domain are inhibitory, and interactions with the Galpha subunit switch II binding surface are stimulatory PMID: 15611108
    21. Fluid shear flow experiments with MG-63 lacking PLC beta2 revealed a significantly higher level of cells losing attachment to coverslips and a significantly lower number of cells increasing intracellular free calcium. PMID: 15939402
    22. Data indicate that PLC-beta2 expression correlates highly with breast cancer malignancy and suggest that it can be included, as an independent marker, among the prognostic indicators in current use. PMID: 16525635
    23. May constitute a molecular marker of breast tumor cells able to monitor the progression to invasive cancers and a target for novel therapeutic breast cancer strategies. PMID: 17429106
    24. PLCB2 activity on actin-associated polyphosphoinositides promotes migration of differentiating tumoral myeloid precursors. PMID: 17478077
    25. Both PLC-beta2 isoforms are expressed at the protein level in platelets. In transfected CV-1 cells, PLC-beta2a is more enriched in the nuclei than PLC-beta2b. The two variants may have different effects in cell proliferation and differentiation. PMID: 17497434
    26. all IGF2 actions were exerted predominantly through IGF2R-linked G(i) protein signaling and required intracellular Ca(2+) mobilization induced by the beta2 isoform of phospholipase C in endothelial progenitor cell homing PMID: 18832656
    27. associated with Libman-Sacks endocarditis, cardiac manifestation of SLE PMID: 19089657
    28. receptor stimulation leads to sequestration of recycling endosomes in a classical protein kinase C- and phospholipase D-dependent manner PMID: 19525236

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  • 数据库链接：

    HGNC: 9055

    OMIM: 604114

    KEGG: hsa:5330

    STRING: 9606.ENSP00000260402

    UniGene: Hs.355888