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PIP5K1A Antibody

  • 货号:
    CSB-PA094535
  • 规格:
    ¥1100
  • 图片:
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA094535(PIP5K1A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA094535(PIP5K1A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
    • Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: PC3 cells, 293T cells, Primary antibody: CSB-PA094535(PIP5K1A Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
  • 其他:

产品详情

  • Uniprot No.:
    Q99755
  • 基因名:
  • 别名:
    68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody; Phosphatidylinositol 4 phosphate 5 kinase type I alpha antibody; Phosphatidylinositol-4-phosphate 5-kinase type I alpha antibody; Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha antibody; PI4P5K Ia antibody; PI51A_HUMAN antibody; PIP5K A antibody; PIP5K1-alpha antibody; Pip5k1a antibody; PIP5KIalpha antibody; PtdIns(4)P-5-kinase 1 alpha antibody
  • 宿主:
    Rabbit
  • 反应种属:
    Human
  • 免疫原:
    Fusion protein of Human PIP5K1A
  • 免疫原种属:
    Homo sapiens (Human)
  • 标记方式:
    Non-conjugated
  • 抗体亚型:
    IgG
  • 纯化方式:
    Antigen affinity purification
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA,WB,IHC
  • 推荐稀释比:
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    WB 1:200-1:1000
    IHC 1:25-1:100
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能:
    Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility. PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3). PIP5K1...显示更多
  • 基因功能参考文献:
    1. KRAS-specific interactor that mediates oncogenic KRAS signaling and proliferation PMID: 30194290
    2. Blockade of IQGAP1 interaction with PIPKIalpha or PI(3)K inhibited PtdIns(3,4,5)P3 generation and signalling, and selectively diminished cancer cell survival. PMID: 27870828
    3. These results indicate that PP1 is recruited to the extracellular calcium-dependent E-cadherin-catenin-PIP5K1a complex in the plasma membrane to activate PIP5K1a, which is required for PLC-g1 activation leading to keratinocyte differentiation. PMID: 27340655
    4. PIP5K1A is modified by polySUMO-2 only during apoptosis. PMID: 23994136
    5. CD28 regulates phosphatidylinositol 4,5-biphosphate turnover by recruiting and activating phosphatidylinositol 4-phosphate 5-kinases alpha in human primary CD4(+) T lymphocytes. PMID: 23589613
    6. Results define the role of PIPKI-alpha in cell migration and describes a new mechanism for the spatial regulation of Rac1 activity that is critical for cell migration. PMID: 20660631
    7. Localized production of Phosphatidylinositol 4,5-bisphosphate by PIP5K1A is required for invadopodia formation by breast cancer cells. PMID: 20426790
    8. Membrane ruffling requires coordination between this enzyme and Rac signaling. PMID: 12682053
    9. in addition to its effects upon Rac activity, Ajuba can also influence cell migration through regulation of PI(4,5)P2 synthesis through direct activation of PIPKIalpha enzyme activity PMID: 15870270
    10. identified apoptotic stimuli that initiate a signaling pathway during cell death that leads to caspase-independent downregulation of PIP5Kalpha. PMID: 16979564
    11. Type I phosphatidylinositol-4-phosphate-5-kinase (PI5KI) alpha and gamma isoforms were identified as the enzymes responsible for PIP2 synthesis in natural killer cells. PMID: 18073347
    12. beta-arrestins direct the localization of PIP5K Ialpha and PIP(2) production to agonist-activated 7TMRs, thereby regulating receptor internalization PMID: 18534983
    13. PI4P5-K Ialpha-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. PMID: 18981107
    14. Extracellular calcium (Cao) stimulated PIP5K1alpha recruitment to the E-cadherin-catenin complex in the plasma membrane. PMID: 19158393
    15. The data suggest that activation of FcgammaRIIA leads to membrane rafts coalescing into signaling platforms containing PIP5-kinase Ialpha and PI(4,5)P(2). PMID: 19331818

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  • 亚细胞定位:
    Cell membrane. Cytoplasm. Nucleus. Nucleus speckle. Cell projection, ruffle. Cell projection, lamellipodium.
  • 组织特异性:
    Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
  • 数据库链接:

    HGNC: 8994

    OMIM: 603275

    KEGG: hsa:8394

    STRING: 9606.ENSP00000357883

    UniGene: Hs.655131