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PHPT1 Antibody

  • 货号：
    CSB-PA017942DA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: PHPT1 antibody at 2µg/ml + MCF-7 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 14 kDa
      Observed band size: 14 kDa
    • Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA017942DA01HU at dilution of 1:100
    • Immunofluorescent analysis of Hela cells using CSB-PA017942DA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PHPT1 Polyclonal antibody
  • Uniprot No.：
    Q9NRX4
  • 基因名：
    PHPT1
  • 别名：
    14 kDa phosphohistidine phosphatase antibody; CGI 202 antibody; HSPC141 antibody; Phosphohistidine phosphatase 1 antibody; PHP14 antibody; PHP14_HUMAN antibody; PHPT1 antibody; Protein janus A homolog antibody; Protein janus-A homolog antibody; Sex regulated protein janus a antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human 14 kDa phosphohistidine phosphatase protein (1-125AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PHPT1 Antibody (CSB-PA017942DA01HU)，的标记方式是Non-conjugated。对于PHPT1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA017942DB01HU PHPT1 Antibody, HRP conjugated ELISA
    FITC CSB-PA017942DC01HU PHPT1 Antibody, FITC conjugated
    Biotin CSB-PA017942DD01HU PHPT1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Exhibits phosphohistidine phosphatase activity.
  • 基因功能参考文献：
    1. In the process of assay optimization, we discovered that PHPT1 is sensitive to a reducing environment and inhibited by transition-metal ions, with one apparent Cu(II) binding site with IC50 value of 500 +/- 20 muM and two apparent Zn(II) binding sites with IC50 values of 25 +/- 1 and 490 +/- 20 muM PMID: 29630837
    2. High PHPT1 expression is associated with cancer. PMID: 29787434
    3. H2O2 exposure induces selective oxidation of hPHPT1 at Met95, a residue within the substrate binding region. Molecular dynamics simulations suggest that if Met95 oxidation alters hPHPT1 activity, then it will do so by altering the stability of an intermediate state. Mass spectrometry showed that H2O2-induced oxidation does not impact hPHPT1 function negatively. PMID: 27034094
    4. PHPT1 was expressed in the epithelium of proximal tubuli and nuclei of clear-cell renal cell carcinoma tissue samples. PMID: 26537769
    5. PHPT1 can dephosphorylate phospholysine in chemically phosphorylated histone H1 and polylysine PMID: 25574816
    6. The specific degradation of the PHPT1 splice variant indicates that the quality control and the self-guarding of the cellular system works at two levels, first at the RNA level and proteasome level. PMID: 25450458
    7. the histidine phosphatase, protein histidine phosphatase 1, inhibits NDPK-B-activated TRPV5 in inside/out patch experiments. PMID: 24523290
    8. Overexpression of PHP by the use of a plasmid vector, pIRES2-AcGFP1-PHP, induced apoptosis in HUVEC PMID: 21967643
    9. Significantly higher expression levels of PHPT1 protein were found in lung cancer samples than in normal tissues adjacent to lung cancer. PMID: 21163124
    10. these data implicate regulatory roles for PHPT1 in a G protein-sensitive step involved in nutrient-induced insulin secretion PMID: 20501872
    11. These results demonstrate for the first time that PHP14 may be functionally important in lung cancer cell migration and the invasion of lung cancer cells, mediated partly through modulation of actin cytoskeleton rearrangement. PMID: 19344975
    12. Northern blot analysis indicated that the human phosphohistidine phosphatase mRNA was present preferentially in heart and skeletal muscle. PMID: 12383260
    13. In the present work, we have searched for potential active site residues in the human phosphohistidine phosphatase by point mutations of conserved histidine and arginine residues to alanine. PMID: 16219293
    14. Overexpression of PHP results in increased dephosphorylation with concomitant inactivation of ACL, thus finally leading to cell damage. PMID: 18656514
    15. PHPT-1 functions to negatively regulate CD4 T cells PMID: 18796614
    16. Solution structure and catalytic mechanism of human protein histidine phosphatase 1. PMID: 18991813
    17. results suggest that an increased activity of protein histidine phosphatase (PHP) impairs neuron cellular function whereas downregulation of PHP does not PMID: 19138678

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Janus family
  • 组织特异性：
    Expressed abundantly in heart and skeletal muscle.
  • 数据库链接：

    HGNC: 30033

    OMIM: 610167

    KEGG: hsa:29085

    STRING: 9606.ENSP00000247665

    UniGene: Hs.409834