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PCMT1 Antibody

  • 货号：
    CSB-PA017618LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA017618LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA017618LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA017618LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PCMT1 Polyclonal antibody
  • Uniprot No.：
    P22061
  • 基因名：
    PCMT1
  • 别名：
    PCMT1 antibody; Protein-L-isoaspartate(D-aspartate) O-methyltransferase antibody; PIMT antibody; EC 2.1.1.77 antibody; L-isoaspartyl protein carboxyl methyltransferase antibody; Protein L-isoaspartyl/D-aspartyl methyltransferase antibody; Protein-beta-aspartate methyltransferase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein-L-isoaspartate(D-aspartate) O-methyltransferase protein (117-228AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PCMT1 Antibody (CSB-PA017618LA01HU)，的标记方式是Non-conjugated。对于PCMT1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA017618LB01HU PCMT1 Antibody, HRP conjugated ELISA
    FITC CSB-PA017618LC01HU PCMT1 Antibody, FITC conjugated
    Biotin CSB-PA017618LD01HU PCMT1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.
  • 基因功能参考文献：
    1. decrease of PCMT1 significantly increased the proportion of D-Asp residues in PHB1 and had significant and fatal impacts on morphology and functions of the mitochondria, such as ATP production and the mitochondrial fusion-fission system PMID: 27327778
    2. PIMT heterozygosity for R36C, G175R, R17H, or R17S would be detrimental to successful aging, whereas homozygosity (should it ever occur) would produce devastating neuropathology PMID: 28100787
    3. Strong PIMT expression was a predictive marker of poor prognosis for surgically resected lung adenocarcinoma. PMID: 26997432
    4. The data of this study indicated that DA-associated PIMT downregulation is an important event contributing to neuronal cell death PMID: 25800307
    5. ERK2-mediated phosphorylation of transcriptional coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic gluconeogenesis. PMID: 24358311
    6. Overexpression of PCMT1 attentuates Mst1 kinase activation and its apoptotic effects in response to hypoxia-induced injury in cardiomyocytes. PMID: 23647599
    7. Data indicate that human PROTEIN ISOASPARTYL METHYLTRANSFERASE (PIMT) can initiate isoAsp conversion to Asp, and is able to restore Arabidopsis PRH75's complex biochemical activity provided isoAsp formation has not led to conformational alterations. PMID: 23903319
    8. Data show six differentially expressed proteins were identified as HSP70, PPIA and alpha-Enolase (up-regulated) S100-A9, PIMT and beta-5 tubulin (down-regulated), most of which had been shown to play a potential role in the pathogenesis of atherosclerosis. PMID: 21839816
    9. The results implied that maternal polymorphisms in PCMT1 might be a potential genetic risk factor for isolated anencephaly in the Chinese population of Lvliang. PMID: 22647835
    10. Study provides new insight into the molecular mechanisms by which PIMT suppresses the p53 activity through carboxyl methylation, and suggests a therapeutic target for cancers. PMID: 22735455
    11. PIMT may act as a co-activator in ERalpha-mediated transcription of TFF1 through its recruitment to the promoter via interacting with ERalpha. PMID: 22382029
    12. Control of PCMT1 expression by microRNA 15a/16-1 may thus represent a late checkpoint in apoptosis regulation PMID: 22033921
    13. A tight cross-regulation exists between ERK and PIMT in regards to their activation and expression during the epithelial mesenchymal transition. PMID: 21841813
    14. study demonstrates a novel role for PIMT as a negative regulator of Abeta peptide formation and a potential protective factor in the pathogenesis of Alzheimer disease PMID: 21372823
    15. Protein L-Isoaspartyl Methyltransferase PMID: 11792715
    16. crystal structure complexed with adenosyl homocysteine (AdoHcy) to 1.6-A resolution PMID: 11847284
    17. Our results showed that the Ile120Val polymorphism of PCMT1 gene is a genetic modifier for the risk of spina bifida. Val/Val genotype was associated with a reduction in risk for spina bifida. PMID: 16256389
    18. A potential role for PIMT in biological processes such as wound healing, cell migration, and tumor metastasis dissemination. PMID: 17167531
    19. These results suggest that PIMT repair of abnormal proteins is necessary to maintain normal MAPK signaling. PMID: 18381200
    20. Four polymorphisms in the protein L-isoaspartyl-O-methyltransferase (PCMT1) gene, encoding a protein repair enzyme, are associated with premature ovarian failure (POF). PMID: 18582870

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Methyltransferase superfamily, L-isoaspartyl/D-aspartyl protein methyltransferase family
  • 数据库链接：

    HGNC: 8728

    OMIM: 176851

    KEGG: hsa:5110

    STRING: 9606.ENSP00000356348

    UniGene: Hs.279257