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PACSIN2 Antibody

  • 货号：
    CSB-PA890765EA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA890765EA01HU diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA890765EA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA890765EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Western Blot
      Positive WB detected in: 293T whole cell lysate, HepG2 whole cell lysate, HL60 whole cell lysate
      All lanes: PACSIN2 antibody at 2.6μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 56, 52 kDa
      Observed band size: 56 kDa
    • Immunoprecipitating PACSIN2 in HepG2 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA890765EA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA890765EA01HU (6μg) + HepG2 whole cell lysate (500μg)
      Lane 3: HepG2 whole cell lysate (20μg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PACSIN2 Polyclonal antibody
  • Uniprot No.：
    Q9UNF0
  • 基因名：
    PACSIN2
  • 别名：
    cytoplasmic phosphoprotein PACSIN2 antibody; OTTHUMP00000198281 antibody; OTTHUMP00000198282 antibody; OTTHUMP00000198283 antibody; OTTHUMP00000198284 antibody; PACN2_HUMAN antibody; Pacsin2 antibody; protein kinase C and casein kinase substrate in neurons 2 antibody; Protein kinase C and casein kinase substrate in neurons protein 2 antibody; SDPII antibody; Syndapin II antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein kinase C and casein kinase substrate in neurons protein 2 protein (240-410AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PACSIN2 Antibody (CSB-PA890765EA01HU)，的标记方式是Non-conjugated。对于PACSIN2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA890765EB01HU PACSIN2 Antibody, HRP conjugated ELISA
    FITC CSB-PA890765EC01HU PACSIN2 Antibody, FITC conjugated
    Biotin CSB-PA890765ED01HU PACSIN2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Regulates the morphogenesis and endocytosis of caveolae. Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus.; (Microbial infection) Specifically enhances the efficiency of HIV-1 virion spread by cell-to-cell transfer. Also promotes the protrusion engulfment during cell-to-cell spread of bacterial pathogens like Listeria monocytogenes. Involved in lipid droplet formation, which is important for HCV virion assembly.
  • 基因功能参考文献：
    1. Pacsin2 recruitment is important for maintenance of cell-cell adhesion by stabilizing the VE-cadherin complex within focal adherens junctions and inhibition of its internalization. PMID: 27417273
    2. observations indicate that PACSIN2 promotes the cell-to-cell spreading of HIV-1 by connecting Gag to the actin cytoskeleton. PMID: 29891700
    3. To our knowledge, this is the first study showing PACSIN2 genotype association with hematological toxicity in ALL patients undergoing maintenance therapy. PMID: 27452984
    4. FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets. PMID: 25838348
    5. our results provide new insights into the mechanism of cellular intoxication by TcdA and the role of PACSIN2 in endocytosis. PMID: 27942025
    6. proposed a packing model for the assembly of PACSIN 2 on membrane, where the proteins are connected by tip-to-tip and wedge loop-mediated lateral interactions on the surface of membrane to generate various diameter tubules PMID: 23888307
    7. PACSIN2 phosphorylation decreases its membrane-binding activity, thereby decreasing its stabilizing effect on caveolae and triggering dynamin-mediated removal of caveolae. PMID: 26092940
    8. PC1 modulates actin cytoskeleton rearrangements and directional cell migration through the Pacsin 2/N-Wasp complex. PMID: 24385601
    9. Cooperation of MICAL-L1, pacsin 2 (syndapin2), and phosphatidic acid in tubular recycling endosome biogenesis. PMID: 23596323
    10. These data indicate that polymorphism in PACSIN2 significantly modulates TPMT activity and influences the risk of GI toxicity associated with mercaptopurine therapy. PMID: 22846425
    11. a novel role for the F-BAR-domain protein PACSIN2 in regulating EGF receptor surface levels and EGF-induced downstream signaling. PMID: 23129763
    12. important role in the formation of plasma membrane caveolae PMID: 21807942
    13. Data identify the BAR-domain protein PACSIN2 as a Rac1 interactor that regulates Rac1-mediated cell spreading and migration. PMID: 21693584
    14. PACSIN2 regulates cell spreading and cell migration, which are dependent on cyclin D1 expression. PMID: 21200149
    15. Results indicate that PACSIN2 mediates membrane sculpting by caveolin-1 in caveola morphology and recruits dynamin-2 for caveola fission. PMID: 21610094
    16. Since the curvature of the neck of the microspike and that of the tubulation share similar geometry, the pacsin2 EFC/F-BAR domain is considered to facilitate both microspike formation and tubulation. PMID: 20188097
    17. Thus, syndapin-dynamin complexes are crucial and sufficient to promote vesicle formation from the trans-Golgi network. PMID: 16551695
    18. These findings suggest a novel function for PACSIN proteins 1, 2, and 3 in dynamic microtubule nucleation. PMID: 18456257
    19. The regulated deformation of membranes and promotion of tubule constrictions by Pacsin suggests a more versatile function of these proteins in vesiculation and endocytosis. PMID: 19549836

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome. Recycling endosome membrane. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Membrane, caveola. Note=Detected at the neck of flask-shaped caveolae. Localization to tubular recycling endosomes probably requires interaction with MICALL1 and EHD1.
  • 蛋白家族：
    PACSIN family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 8571

    OMIM: 604960

    KEGG: hsa:11252

    STRING: 9606.ENSP00000263246

    UniGene: Hs.162877