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NXF1 Antibody

  • 货号：
    CSB-PA004228
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of HepG2 cells using TAP Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q9UBU9
  • 基因名：
    NXF1
  • 别名：
    DKFZp667O0311 antibody; DmNXF1 antibody; MEX67 antibody; MEX67, yeast, homolog of antibody; Mex67p antibody; mRNA export factor TAP antibody; Mvb1 antibody; Nuclear RNA export factor 1 antibody; Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody; nxf 1 antibody; NXF1 antibody; NXF1_HUMAN antibody; Protein small bristles antibody; Sbr antibody; TAP antibody; Tip associating protein antibody; Tip-associated protein antibody; Tip-associating protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from the N-terminal region of Human TAP.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:40000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.
  • 基因功能参考文献：
    1. Gain or loss-of-function experiments revealed NXF1 selectively regulates TLR7-driven IRF5 transcriptional activity, suggesting a new role for NXF1 in the IRF5 signaling pathway. PMID: 28578407
    2. repeat RNA-sequestration of SRSF1 triggers the NXF1-dependent nuclear export of C9ORF72 transcripts retaining expanded hexanucleotide repeats PMID: 28677678
    3. Hepatitis B virus core protein associates with the cellular NXF1-p15 complex via the nuclear export signal motif of hepatitis B virus core protein.Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the NXF1-p15 machinery. PMID: 25360769
    4. Complementary structural, biochemical and cellular techniques indicated that the formation of a symmetric RNA binding platform generated by dimerization of NXF1:NXT1 facilitates the recognition of CTE-RNA and promotes its nuclear export PMID: 25628361
    5. Gag polyprotein synthesis was decreased by NXF1 knockdown. PMID: 25212909
    6. TREX is the major complex used to recruit Nxf1 to mRNA in human cells. PMID: 22893130
    7. the organization of the NXF1 proline-tyrosine nuclear localization signal reveal unexpected redundancy in the nuclear import pathways used by NXF1. PMID: 21965294
    8. The U2AF65 protein served as an adaptor to link expanded CAG RNA to NXF1 for RNA export. PMID: 21725067
    9. The crystal structure of the RNA recognition and leucine-rich repeat motifs of TAP bound to one symmetrical half of the CTE RNA is solved. PMID: 21822283
    10. these data revealed a redox-regulated chaperone function of PDI in delivering antigenic peptides from TAP to MHC-I. PMID: 21299467
    11. Tpr plays an important role in quality control of mRNA trafficked on the Nxf1 pathway. PMID: 21613532
    12. These results showed that the interaction between herpes simplex virus type 1 ICP27 and human TAP/NXF1 occurred in living cells upon head-to-tail intramolecular association of ICP27. PMID: 21060739
    13. Varicella-zoster virus IE4 protein interacts with SR proteins and exports mRNAs through the TAP/NXF1 pathway PMID: 19924249
    14. Depletion of NXF1 or 5,6-dichloro-1-beta-d-ribofuranosyl-benzimidazole treatment had similar effects, inhibiting the nuclear export of several of the H5N1 influenza virus mRNAs. PMID: 20071484
    15. The structural and mutational data define a novel RNA-binding site on the Tap protein PMID: 11854490
    16. Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1 PMID: 11875519
    17. Karyopherin beta2B is a nuclear export factor and mediates mRNA export in cooperation with TAP PMID: 12384575
    18. structural basis for interaction of UBA domain and FG nucleoporins PMID: 12581645
    19. nuclear export sequences regulate human TAP function in mRNA export by direct nuclear pore interactions PMID: 14963046
    20. hypophosphorylation of ASF in mRNPs coincides with its higher affinity for TAP PMID: 15184380
    21. SR proteins 9G8 and ASF/SF2 exhibit higher affinity for TAP/NXF1 when hypophosphorylated PMID: 15210956
    22. concluded that herpes simplex virus type 1 ICP27 and the viral RNAs to which it binds are exported via the TAP/NXF1 export receptor. PMID: 15767397
    23. results indicate that Tap regulates expression of its own intron-containing RNA through a CTE (constitutive transport element)-mediated mechanism PMID: 16971948
    24. RNA transport element RTE evolved as a high affinity RBM15 ligand to provide a splicing-independent link to NXF1, thereby ensuring efficient nuclear export and expression of retrotransposon transcripts PMID: 17001072
    25. evidence presented that the export factor TAP/NXF1 binds poorly to XIST RNA in comparison to exported mRNAs, suggesting that reduced TAP/NFX1 binding may contribute to nuclear retention of XIST RNA PMID: 17333237
    26. ORF57 interacts with the cellular export factor REF and with RNA, suggesting that it may provide target mRNAs with access to REF, which mediates nuclear RNA export by binding to TAP/NXF1. PMID: 17609285
    27. Homotypic Tap complex can interact with both Nxt1 and nucleoporins in vitro. PMID: 17978099
    28. The efficiency and stability of the approach are demonstrated by reconstructing the structure of a two domain region of the 31 kDa nuclear export factor TAP (TIP-associated protein). PMID: 18670889
    29. ICP27 is the major export adaptor for HSV-1 mRNA and that it links bound transcripts to the TAP/NXF1 export receptor PMID: 19019956
    30. The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1. PMID: 19586903
    31. oligomerization of Tap affects its interactions with nucleoporins PMID: 17978099

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleoplasm. Nucleus speckle. Nucleus, nuclear pore complex. Nucleus envelope. Cytoplasm. Cytoplasm, Stress granule.
  • 蛋白家族：
    NXF family
  • 组织特异性：
    Expressed ubiquitously.
  • 数据库链接：

    HGNC: 8071

    OMIM: 602647

    KEGG: hsa:10482

    STRING: 9606.ENSP00000294172

    UniGene: Hs.523739