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NQO2 Antibody

  • 货号：
    CSB-PA016040LA01HU
  • 规格：
    ￥3,900
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, A549 whole cell lysate, MCF7 whole cell lysate, ntera2 whole cell lysate, Mouse Kidney tissue lysate, Mouse Heart tissue lysate
      All lanes: NQO2 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size:26 kDa
      Observed band size:26 kDa
    • IHC image of CSB-PA016040LA01HU diluted at 1:150 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA016040LA01HU diluted at 1:150 and staining in paraffin-embedded human breast tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA016040LA01HU diluted at 1:150 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Immunofluorescence stainingof Hela cell with CSB-PA016040LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence stainingof U251 cell with CSB-PA016040LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    To prepare the NQO2 polyclonal antibody, the recombinant human NQO2 protein (1-231aa) is used to immunize a rabbit. The rabbit's immune system will recognize the immunogen as foreign and produce antibodies against it. The NQO2 polyclonal antibodies are purified from the rabbit's serum using protein G affinity chromatography. The purity of this NQO2 antibody is over 95%. This NQO2 antibody can be used to detect human and mouse NQO2 proteins in multiple applications, including ELISA, WB, IHC, IF, and IP.

    NQO2 protein mainly functions to catalyze the reduction of quinones, which are harmful compounds that can cause oxidative damage to cells. NQO2 also plays a role in the metabolism of drugs, toxins, and carcinogens, and is involved in other cellular processes such as regulation of gene expression, cell proliferation, and apoptosis. Mutations in the NQO2 gene have been linked to several diseases, such as cancer and Parkinson's disease.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) NQO2 Polyclonal antibody
  • Uniprot No.：
    P16083
  • 基因名：
    NQO2
  • 别名：
    DHQV antibody; DIA6 antibody; EC 1.10.99.2 antibody; MGC94180 antibody; NAD(P)H dehydrogenase quinone 2 antibody; NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 antibody; NAD(P)H menadione oxidoreductase 2 dioxin inducible antibody; NMOR2 antibody; NQO 2 antibody; NQO2 antibody; NQO2_HUMAN antibody; NRH dehydrogenase [quinone] 2 antibody; NRH dehydrogenase antibody; NRH:quinone oxidoreductase 2 antibody; OTTHUMP00000015948 antibody; OTTHUMP00000015949 antibody; OTTHUMP00000015953 antibody; Ox 2 antibody; Ox2 antibody; QR2 antibody; Quinone antibody; Quinone reductase 2 antibody; Ribosyldihydronicotinamide dehydrogenase [quinone] antibody; Ribosyldihydronicotinamide dehydrogenase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Ribosyldihydronicotinamide dehydrogenase [quinone] protein
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，NQO2 Antibody (CSB-PA016040LA01HU)，的标记方式是Non-conjugated。对于NQO2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA016040LB01HU NQO2 Antibody, HRP conjugated ELISA
    FITC CSB-PA016040LC01HU NQO2 Antibody, FITC conjugated
    Biotin CSB-PA016040LD01HU NQO2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.02% sodium azide
    Constituents: PBS containing 50% glycerol pH 7.3
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:4000
    IHC 1:100-1:300
    IF 1:20-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • 基因功能参考文献：
    1. The ontogeny and population variability of human hepatic dihydronicotinamide riboside:quinone oxidoreductase (NQO2). PMID: 28346733
    2. In patients with Alzheimer's disease, QR2 is overexpressed in the insular cotex. PMID: 26609153
    3. NQO2-L47 is less stable towards proteolytic digestion and thermal denaturation than NQO2-F47. PMID: 24631540
    4. NQO1 609C>T and NQO2 -3423G>A polymorphisms do not seem to play any significant role in susceptibility or prognosis of EC in north Indian population. PMID: 22770696
    5. This first structure of a reduced quinone reductase shows that reduction of the FAD cofactor and binding of a specific inhibitor lead to global changes in NQO2 structure and is consistent with a functional role for NQO2 as a flavin redox switch. PMID: 23471972
    6. both NQO1 and NQO2 modulate the efficacy of AC therapy and that NQO2 is associated with tamoxifen toxicity. PMID: 21946896
    7. results indicate a role of NQO2 in the control of AKT/GSK-3beta/cyclin D1 and highlight the involvement of NQO2 in degradation of cyclin D1, as part of mechanism of chemoprevention by resveratrol PMID: 22266466
    8. Results suggest that NQO2, SOD2 and SOD3 may significantly modify prognosis of breast cancer patients. PMID: 21351093
    9. results suggest that the increase in hippocampal QR2 might be a cause of AD or might promote the progression of AD by causing an increase in the toxic quinone levels and consequent loss of cognitive function. PMID: 21803122
    10. Selective inhibition of NQO2 over NQO1 can be attributed to the bulky aminoalkylamino substituent at the 5-position, which blocks entrance of the indolequinones into the NQO1 active site. PMID: 21718050
    11. NQO2 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
    12. The NQO1 609C>T TT genotype and T allele were significantly associated with increased risk for gastric cancer (GC), whereas NQO2 -3423G>A polymorphism did not show any association with GC. PMID: 21294640
    13. The first European study of the SOD2, SOD3, NQO1, and NQO2 roles in pancreatic cancer etiology did not find significant associations. PMID: 20966810
    14. We observed a nominal significant association between the rs1143684 NQO2 polymorphism and the trajectory of delayed memory recall over time (p=0.029). No other associations were seen with the decline of other cognitive abilities. PMID: 18538895
    15. Our findings challenge the previous assumption that the NQo2 I-16 allele is stable only when paired with another I-16 allele PMID: 19495956
    16. polymorphisms associated with idiopathic Parkinson's disease PMID: 11688992
    17. A significant difference was found between alcoholic patients and controls in genotype frequency at an insertion/deletion site in the promoter region of the NQO2 gene (p = 0.0014). PMID: 12960511
    18. the level of NQO2 mRNA is low in AGR patients compared with the control group. Such a reduction in message suggests that the NQO2 gene may be involved in the development of clozapine-induced AGR. PMID: 14617031
    19. NQO2 gene may confer susceptibility to a certain form of schizophrenia. PMID: 14639047
    20. the NQO2 gene is differentially expressed by the polymorphic promoters in human cells transfected with NQO2 gene reporter constructs. Alterations in NQO2 activity might be an important factor in susceptibility to Parkinson's disease. PMID: 15451063
    21. We propose that induction of NQO2 may relate to the observed increased expression of p53 that, in turn, contributes to the observed suppression of cell growth in both melanoma cell lines. PMID: 15993843
    22. the nicotinamide adenine dinucleotide phosphate reduced-diaphorase interneurons in the human striatum. PMID: 16025450
    23. The results combined demonstrated that Nrf2 associates with JunD, binds to ARE at nucleotide -1433, and regulates human NQO2 gene expression and induction in response to antioxidants. PMID: 16545679
    24. an apparent association between an NQO2 exon 3 single-nucleotide polymorphism and lower enzymatic activity PMID: 17332305
    25. The crystal structure of melatonin and 2-iodomelatonin in complex with QR2 provide a detailed description of the enzyme active site; the design of inhibitors of the enzyme might be instrumental in discriminating the role of MT1, MT2 and QR2/MT3 PMID: 18254726
    26. Parkinson's disease patients were analyzed for NQO2 gene promoter polymorphisms. Three allelic variants were I-29, I-16 and D. The association of the D promoter with Parkinson's disease may be due to an increase in expression of the NQO2 gene. PMID: 18314446
    27. This first structural analysis of hQR2 should enable to better understand the biological role of melatonin on this enzyme . PMID: 18502195
    28. Quinone reductase 2 is a catechol quinone reductase. quinone reductase 2 could play important roles in the regulation of catecholamine oxidation processes that may be involved in the etiology of Parkinson disease. PMID: 18579530
    29. nmr studies of QR2 catalytic reaction were performed; results led to conclusion that melatonin is not cleaved to form N1-acetyl-N2-formyl-5-methoxykynurenine by a catalytically active QR2, indicating melatonin is neither a substrate nor a co-substrate PMID: 18826489
    30. NQO2 is a susceptibility gene for breast carcinogenesis. PMID: 19351655

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    NAD(P)H dehydrogenase (quinone) family
  • 数据库链接：

    HGNC: 7856

    OMIM: 160998

    KEGG: hsa:4835

    STRING: 9606.ENSP00000337773

    UniGene: Hs.533050