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  4. NOTCH4 Antibody

NOTCH4 Antibody

  • 货号：
    CSB-PA446675
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA446675(NOTCH4 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human liver cancer tissue, Human kidney tissue, Primary antibody: CSB-PA446675(NOTCH4 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
  • 其他：

产品详情

  • Uniprot No.：
    Q99466
  • 基因名：
  • 别名：
    FLJ16302 antibody; hNotch 4 antibody; hNotch4 antibody; INT3 antibody; Neurogenic locus notch homolog protein 4 antibody; NOTC4_HUMAN antibody; Notch 4 antibody; Notch 4 intracellular domain antibody; Notch homolog 4 antibody; Notch4 antibody; NOTCH4 protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthetic peptide of Human NOTCH4
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:10000
    WB 1:500-1:2000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

问答及客户评论

■ 常见问题解答
Q:

我了解到抗体CSB-PA446675的免疫原是人NOTCH4的合成肽。免疫原是细胞内部分还是细胞外部分?

A:
抗体CSB-PA446675针对人NOTCH4的1969 －2003氨基酸建立，能识别NOTCH4的胞质结构域。提供的免疫原序列如下: 免疫原区域:1969-2003 amino acids of human NOTCH4 氨基酸序列:　ppcltpspergspqldcgppalqempinqgegkk

靶点详情

  • 功能：
    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
  • 基因功能参考文献：
    1. Results provide evidence that Notch4 plays an important role in vasculogenic mimicry formation and tumor invasion in hepatocellular carcinoma. PMID: 29058285
    2. The results of the present study show that RNA interference targeting against Notch-4 expression suppresses prostate cancer progression. PMID: 28374086
    3. Very rare missense variant in the regulatory NODP domain of NOTCH4 located at the 6p21 locus was identified in a single family.This family suggests a novel mechanism of systemic sclerosis pathogenesis in which a rare and penetrant coding variation can substantially elevate disease risk in contrast to the more modest non-coding variation typically found at this locus. PMID: 27829420
    4. High NOTCH4 expression is associated with preeclampsia. PMID: 27069008
    5. Data suggest that the vascular DLL4-Notch4 signaling and VEGF signaling complementing each other plays an important role in the progression of tumor angiogenesis in primary glioblastoma. PMID: 26472724
    6. findings indicate that Notch4+ melanoma cancer stem-like cells trigger epithelial-mesenchymal transition and promote the metastasis of melanoma cells PMID: 27234159
    7. the aberrant activation of Notch4 promotes oral squamous cell carcinoma metastasis. PMID: 27197026
    8. data are consistent with those obtained in previous studies that suggested that rs204993 is associated with schizophrenia and that the AA genotype of rs204993 demonstrates a higher risk PMID: 26605328
    9. High Notch4 expression is associated with Prostatic Intraepithelial Neoplasia. PMID: 26341090
    10. Anti-estrogen resistance in human breast tumors is driven by JAG1-NOTCH4-dependent cancer stem cell activity. PMID: 26387946
    11. NOTCH4 polymorphism can influence clinical symptoms in Slovenian patients with schizophrenia. PMID: 25529856
    12. Our findings provide a novel mechanism for Notch4-ICD regulation, suggesting a negative regulatory role for the PI3K-AKT pathway in Notch4 nuclear signaling. PMID: 25740432
    13. This study failed to replicate the significant association between the NOTCH4 gene rs3131296 polymorphism and the risk for schizophrenia. PMID: 25142293
    14. BITC-mediated inhibition of bCSC was augmented by knockdown of Notch4 and Nicastrin, but not by RNA interference of Notch1 or Notch2. PMID: 25663545
    15. Study shows that Notch4 activation can promote gastric cancer growth by inducing Wnt1/b-catenin signaling. PMID: 25511451
    16. A genome-wide significant association with cryoglobulin-related vasculitis was identified with SNPs near NOTCH4. PMID: 25030430
    17. This paper demonstrated that Nicastrin and Notch4 are key molecules involved in resistance to endocrine therapy. The data suggest that targeting Notch4 and Nicastrin is a potential approach to reverse endocrine resistance in breast cancer patients. PMID: 24919951
    18. The associations of rs2616984 in CSMD1 gene, putative associations of rs3131296 in NOTCH4 gene, and associations of rs2229741 of NRIP1 gene with Alzheimer's disease have been found in a Russian population. PMID: 25845235
    19. The expression of NOTCH4 in the dorsolateral prefrontal cortex and amygdala is decreased in suicide victims compared to control brain. PMID: 24151014
    20. High Notch4 expression is associated with glioblastoma multiforme. PMID: 24305720
    21. This NOTCH4 missense mutation decreased the risk for developing multiple sclerosis in Japanese, but did not affect clinical features of those who had already developed the disease PMID: 23549433
    22. A possible contribution of Notch4 signalling to the development of brain arteriovenous malformations in human. PMID: 24373503
    23. NOTCH4 overexpression is associated with triple-negative breast cancer. PMID: 24403446
    24. RNAi directed against Notch-4 markedly decreased Notch-4 gene expression. PMID: 23450325
    25. The results of this study provided stringent support for previous genetic findings reporting the association of an intronic SNP (rs3131296, chr6: 32 172 993 bp on hg19) in NOTCH4 in a European cohort. PMID: 22641179
    26. The length of (CA)n in EGFR and (CTG)n in NOTCH4 was not associated with breast cancer (OR=0.99; 95% CI 0.59-1.37; p=0.619 and OR=1.08; 95% CI 0.71-1.65; p=0.725, respectively). PMID: 23015403
    27. Our study shows that genetic variations within the NOTCH4 gene significantly modify the clinical characteristics of migraine and may have a role in disease pathogenesis. PMID: 23566281
    28. We provide the first genetic evidence suggesting the involvement of NOTCH4 polymorphisms in rheumatoid inflammatory process. PMID: 23318300
    29. Notch4-dependent antagonism of canonical TGF-beta1 signaling defines unique temporal fluctuations of SMAD3 activity in proximal tubular epithelial cells exposed to shear stress. PMID: 23576639
    30. These data suggest that N4IC promoted proliferation through modulating the ERK pathway and the cell cycle during the early stage of 3T3-L1 adipogenesis and facilitated differentiation through up-regulating adipogenic genes such as C/EBPalpha, PPARgamma, aP2, LPL and HSL during the middle and late stages of 3T3-L1 adipogenesis. PMID: 23237809
    31. strong association between expression and bipolar disorder PMID: 23212060
    32. Genome-wide association study of age-related macular degeneration identifies TNXB, FKBPL and NOTCH4 as candidate susceptibility genes. PMID: 22694956
    33. Functional interaction of Notch4(ICD) and Elongin C provides novel insight into regulation of Notch signaling in epithelial cell biology and disease. PMID: 22001063
    34. the NOTCH4 polymorphisms, the minor allele of rs422951 and the 12-repeat variation for rs72555375, can be considered to be markers for sporadic inclusion body myositis susceptibility PMID: 22732452
    35. The NOTCH4 polymorphisms most strongly associated with schizophrenia exert their effects on susceptibility by altering the efficiency and/or alternative splicing of Notch4 transcripts. PMID: 22488909
    36. arachidonic acid significantly decreased methylation of the promoters of both KDR and Notch4 genes in Human umbilical vein endothelial cells. PMID: 21864059
    37. Only rs17604492 single nucleotide polymorphism of NOTCH4 gene was found to be weakly associated with susceptibility to Kawasaki Disease (KD) in a Korean population. Results indicate NOTCH4 doesn't contribute to susceptibility to KD. PMID: 21203870
    38. This research examined the methylation status of a region surrounding the NOTCH4 -25 C/T site in leukocyte genomic DNA and human brain regions. PMID: 21085055
    39. Studies indicate that different receptors and ligands expression in breast cancer and various ways in which the pathway can be inhibited. PMID: 21045140
    40. Notch-4 may play an important role in salivary adenoid cystic carcinoma. PMID: 20596622
    41. Notch 4 receptors and their ligands play differential roles in the cytodifferentiation of squamous odontogenic tumors of the mandible. PMID: 20554499
    42. Notch4 signaling activity was 8-fold higher in breast cancer stem cell-enriched cell populations compared with differentiated cells, whereas Notch1 signaling activity was 4-fold lower in the stem cell-enriched cell populations. PMID: 20068161
    43. The Notch gene family regulates embryonic cell migration, vascular morphogenesis and remodeling. The Notch 4 locus has been implicated in schizophrenia, but this study rules out a significant role for the Notch4 gene in schizophrenia in the Han Chinese. PMID: 11803454
    44. Genetic analysis of (CTG)n polymorphism in bipolar pedigrees PMID: 11901359
    45. Activated Notch4 inhibits angiogenesis: role of beta 1-integrin activation. PMID: 11909975
    46. significant association of alopecia areata in the overall data set with the Notch4(T+1297C) polymorphism (P<0.001) but not with Notch4(A+3063G) PMID: 12589427
    47. The investigated NOTCH4 variants do not have a major influence on susceptibility to schizophrenia or related neurobiological traits. PMID: 12605097
    48. Findings may reflect the interaction of NOTCH4 with the underlying genetic and phenotypic complexity that characterizes both schizophrenia and normal cognition and brain development. PMID: 12627456
    49. NOTCH4 T-25C polymorphism has an important effect on the age of onset in schizophrenia. PMID: 12782960
    50. Notch4 signaling in vascular endothelial cells. PMID: 12814948

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.; [Notch 4 intracellular domain]: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
  • 蛋白家族：
    NOTCH family
  • 组织特异性：
    Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukoc
  • 数据库链接：

    HGNC: 7884

    OMIM: 164951

    KEGG: hsa:4855

    STRING: 9606.ENSP00000364163

    UniGene: Hs.436100