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  4. NEK1 Antibody

NEK1 Antibody

  • 货号：
    CSB-PA444209
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human lymphoma tissue using CSB-PA444209(NEK1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA444209(NEK1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q96PY6
  • 基因名：
    NEK1
  • 别名：
    DKFZp686D06121 antibody; DKFZp686K12169 antibody; kat antibody; KIAA1901 antibody; MGC138800 antibody; MGC189817 antibody; NEK1 antibody; NEK1_HUMAN antibody; Never in mitosis A-related kinase 1 antibody; Never in mitosis gene A-related kinase 1 antibody; NIMA (never in mitosis gene a)-related kinase 1 antibody; NIMA-related kinase 1 antibody; NimA-related protein kinase 1 antibody; NY-REN-55 antibody; OTTHUMP00000219432 antibody; OTTHUMP00000219433 antibody; OTTHUMP00000219434 antibody; OTTHUMP00000219435 antibody; Protein-serine/threonine kinase antibody; Renal carcinoma antigen NY-REN-55 antibody; Serine/threonine-protein kinase Nek1 antibody; SRPS2 antibody; SRPS2A antibody; SRTD6 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthetic peptide of Human NEK1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Involved in DNA damage checkpoint control and for proper DNA damage repair. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death. May be implicated in the control of meiosis. Involved in cilium assembly.
  • 基因功能参考文献：
    1. We provide novel evidence for association of NEK1 with ALS in Chinese, reporting variants in these genes not previously found in Europeans. PMID: 29149916
    2. NEK1 variants may modify disease presentation of driving mutations. PMID: 28935222
    3. Following DNA damage, addition of the TLK1 inhibitor, THD, or overexpression of NEK1-T141A mutant impaired ATR and Chk1 activation, indicating the existence of a TLK1>NEK1>ATR>Chk1 pathway. Indeed, overexpression of the NEK1-T141A mutant resulted in an altered cell cycle response after exposure of cells to oxidative stress, including bypass of G1 arrest and implementation of an intra S-phase checkpoint. PMID: 28426283
    4. This study shown that the NEK1 is a novel in patient with familial amyotrophic lateral sclerosis. PMID: 26945885
    5. Mutation in NEK1 gene is associated with amyotrophic lateral sclerosis. PMID: 27455347
    6. Nek1 phosphorylates Rad54 and regulates Rad51 removal to orchestrate homologous recombination and replication fork stability. PMID: 27264870
    7. Compound heterozygous variants in NEK1 were identified in two brothers with Mohr syndrome. The nonsense variant c.1226G>A, p.(Trp409*), results in nonsense-associated alternative splicing. Ciliation in patient fibroblasts is drastically reduced. PMID: 27530628
    8. The skeletal phenotype of our patient was milder than those of previously reported cases with NEK1 mutations and those with axial SMD harboring C21orf2 mutations. Phenotypes associated with NEK1 mutations are variable and the phenotype-genotype corelation in skeletal ciliopathies is challenging PMID: 28123176
    9. Nek1 overexpression in gliomas was correlated with the proliferation marker (Ki-67), tumor grade, Karnofsky performance scale (KPS) and more importantly, patients' poor survival. Further studies showed that Nek1 expression level was also increased in multiple glioma cell lines (U251-MG, U87-MG, U118, H4 and U373). PMID: 27251576
    10. C21ORF2 functions in the same pathway as NEK1 in DNA damage repair. PMID: 26290490
    11. Nek1 may facilitate S-phase progression by interacting with Ku80 and regulating chromatin loading of replication factors. PMID: 23851348
    12. Nek1 may suppress cilia by phosphorylating pVHL, which is critical to microtubule stabilization and ciliary stability. PMID: 23255108
    13. as an ATR-associated kinase, Nek1 enhances the stability and activity of ATR-ATRIP before DNA damage, priming ATR-ATRIP for a robust DNA damage response PMID: 23345434
    14. The present case provides evidence for a correlation of NEK1 mutation with short rib-polydactyly syndrome type II (Majewski. PMID: 22482978
    15. This study confirms that NEK1 is one gene causing SRP type II but also reports mutations in DYNC2H1, expanding the phenotypic spectrum of DYNC2H1 mutations. PMID: 22499340
    16. results demonstrate that Nek1 is important for proper checkpoint control and characterize for the first time a DNA damage response that does not directly involve one of the known upstream mediator kinases, ATM or ATR PMID: 21301226
    17. absence of functional full-length NEK1 severely reduces cilia number and alters ciliar morphology in vivo. PMID: 21211617
    18. Nek1 is involved in the beginning of the cellular response to genotoxic stress and plays an important role in preventing cell death induced by DNA damage. PMID: 20501547
    19. Data report that NEK1 and CLASP2 colocalize with FEZ1 in a perinuclear region in mammalian cells, and observed that coiled-coil interactions occur between FEZ1/CLASP2 and FEZ1/NEK1 in vitro. PMID: 19924516
    20. these data provide a mechanism to explain how Nek1 regulates cell death by affecting the opening and closing of VDAC1. PMID: 20230784
    21. Mapping studies of NEK1 regulatory domain indicate interaction with most of the proteins known to take part in etiology of polycystic kidney disease, in double-strand DNA break repair at the G2/M cell-cycle transition phase, or in neural cell development. PMID: 14690447
    22. Nek1 may function as a kinase early in the DNA damage response pathway. PMID: 15604234
    23. These data show that Nek1 is important for efficient DNA damage checkpoint control and for proper DNA damage repair. PMID: 18843199

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  • 相关疾病：
    Short-rib thoracic dysplasia 6 with or without polydactyly (SRTD6)
  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
  • 组织特异性：
    High fetal expression in the brain and kidney.
  • 数据库链接：

    HGNC: 7744

    OMIM: 263520

    KEGG: hsa:4750

    STRING: 9606.ENSP00000424757

    UniGene: Hs.481181