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NDN Antibody

  • 货号：
    CSB-PA859515LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA859515LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA859515LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) NDN Polyclonal antibody
  • Uniprot No.：
    Q99608
  • 基因名：
    NDN
  • 别名：
    HsT16328 antibody; NDN antibody; NECD_HUMAN antibody; Necdin antibody; Necdin homolog antibody; Necdin MAGE family member antibody; necdin related protein antibody; PWCR antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Necdin protein (1-98AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，NDN Antibody (CSB-PA859515LA01HU)，的标记方式是Non-conjugated。对于NDN Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA859515LB01HU NDN Antibody, HRP conjugated ELISA
    FITC CSB-PA859515LC01HU NDN Antibody, FITC conjugated
    Biotin CSB-PA859515LD01HU NDN Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.
  • 基因功能参考文献：
    1. the single-nucleotide polymorphism rs850807, which is putatively functional and linked with MAGEL2 and NDN Genetic variation in rs850807 was strongly and exclusively associated with the ideas of reference subscale of the schizophrenia spectrum, which is best typified as paranoia PMID: 29343559
    2. this study shows that hypermethylation of NDN promotes cell proliferation by activating the Wnt signaling pathway in colorectal cancer PMID: 28521288
    3. One candidate variant was located in an alpha helix of Necdin (NDN), phased to the paternally inherited allele. NDN is maternally imprinted within the 15q11.2 Prader-Willi Syndrome (PWS) region PMID: 28213671
    4. NDN is an imprinted tumor suppressor gene which affects cancer cell motility, invasion and growth and that its loss of function in ovarian cancer can be caused by both genetic and epigenetic mechanisms. PMID: 26689988
    5. Germline single nucleotide polymorphism in necdin gene is associated with breast cancer. PMID: 26384308
    6. NDN and CD1A are novel prognostic methylation markers in patients with head and neck squamous carcinomas PMID: 26518708
    7. Necdin expression declined during replicative aging of IMR90 primary human fibroblasts or after induction of premature senescence.Showed that in normal human cells, Necdin expression mimicked the effect of p53 inactivation by increasing radioresistance. PMID: 22691188
    8. Hypermethylation and mutation of necdin is associated with neoplasms. PMID: 23549060
    9. necdin exerts its pro-survival activity by counteracting the action of the pro-apoptotic protein Cell Cycle Apoptosis Regulatory Protein (CCAR1/CARP1) PMID: 22905258
    10. necdin has multiple roles within protein complexes in different subcellular compartments, and indicate that it can utilize multiple karyopherin-dependent pathways to modulate its localization. PMID: 22442722
    11. In pre-adipocytes, necdin over-expression inhibits adipogenesis, while reducing necdin levels enhances adipogenic differentiation in tissue culture cell PMID: 22305984
    12. Necdin, a negative growth regulator,identified as a novel STAT3 target gene, whose expression is down-regulated at the mRNA and protein levels when STAT3 is constitutively active. PMID: 22046235
    13. Necdin is implicated through the TNF-receptor 1 pathway in the developmental death of motoneuron PMID: 21912643
    14. rare variant of necdin (p.V318A) was described in a family with Kallmann syndrome Familial segregation and in vitro analysis suggested that this non-synonymous variant did not have a direct causative role in hypogonadism phenotype. PMID: 21543378
    15. Data suggest that the effects of necdin deletion on the developing nervous system may depend on the relative expression of p75NTR and TrkA in the cells of particular regions of the nervous system. PMID: 21150695
    16. Necdin is a key protein regulating polarization of the cytoskeleton and myosin activation during development. PMID: 20665884
    17. The necdin gene is imprinted, with preferential expression from the paternal allele in human and mouse. PMID: 9302265
    18. the constitutively up-regulated expression of pre-IL-1 alpha in the nuclei of systemic sclerosis (SSc) fibroblasts up-regulates proliferation and matrix production of SSC fibroblasts through binding necdin PMID: 12913118
    19. Tissue-specific gene expression regulation and imprinted epigenetic modifications of the human NDN gene. PMID: 15247330
    20. Necdin can be a novel negative regulator of HIF-1alpha stability via the direct interaction. PMID: 15978586
    21. Lack of Necdin expression induces perinatal serotonergic alterations that affect the maturation and function of the respiratory network, inducing breathing deficits in mice and probably in Prader-Willi patients. PMID: 18272695
    22. Data suggest that Nogo-A is a novel necdin binding protein and inhibits necdin-accelerated neuronal neurite outgrowth by sequestering necdin in the cytoplasm. PMID: 19386232
    23. regulates neuronal development. (review) PMID: 19517793
    24. Confirmation of NDN as a tumor suppressor may have implications for monitoring of PWS patients and could present a novel cancer therapeutic target PMID: 19626646

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  • 亚细胞定位：
    Perikaryon. Nucleus. Note=Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
  • 组织特异性：
    Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leuko
  • 数据库链接：

    HGNC: 7675

    OMIM: 176270

    KEGG: hsa:4692

    STRING: 9606.ENSP00000332643

    UniGene: Hs.50130