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NAE1 Antibody

  • 货号：
    CSB-PA613411ESR1HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: NAE1 antibody at 1.85µg/ml
      Lane 1: Mouse liver tissue
      Lane 2: Mouse kidney tissue
      Lane 3: Mouse skeletal muscle tissue
      Lane 4: 293T whole cell lysate
      Lane 5: A431 whole cell lysate
      Lane 6: K562 whole cell lysate
      Lane 7: Hela whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 61, 60, 51 kDa
      Observed band size: 61 kDa
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA613411ESR1HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) NAE1 Polyclonal antibody
  • Uniprot No.：
    Q13564
  • 基因名：
    NAE1
  • 别名：
    59 kDa antibody; A 116A10.1 antibody; Amyloid beta precursor protein binding protein 1 59kD antibody; Amyloid beta precursor protein binding protein 1 59kDa antibody; Amyloid beta precursor protein binding protein 1 antibody; Amyloid beta precursor protein binding protein 1, 59 kDa antibody; Amyloid beta precursor protein-binding protein 1 antibody; Amyloid protein binding protein 1 antibody; Amyloid protein-binding protein 1 antibody; APP BP1 antibody; APP-BP1 antibody; APPBP 1 antibody; APPBP1 antibody; HPP 1 antibody; HPP1 antibody; NAE 1 antibody; NAE1 antibody; NEDD8 activating enzyme E1 regulatory subunit antibody; NEDD8 activating enzyme E1 subunit 1 antibody; NEDD8 activating enzyme E1 subunit antibody; NEDD8-activating enzyme E1 regulatory subunit antibody; Proto oncogene protein 1 antibody; Proto-oncogene protein 1 antibody; Protooncogene protein 1 antibody; ula 1 antibody; ULA1_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human NEDD8-activating enzyme E1 regulatory subunit protein (1-220AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
  • 基因功能参考文献：
    1. Here we report that high expression of neddylation components, NEDD8 and NAE1, are associated with poor survival of Pancreatic ductal adenocarcinoma patients. Blockage of neddylation by MLN4924 significantly sensitizes pancreatic cancer cells to gemcitabine, reduced clonogenic survival, decreased invasion capacity, increased apoptosis and senescence PMID: 28535453
    2. E1 (NAE1 and UBA3) and E2 (UBC12) enzymes, as well as global NEDD8 conjugation, were upregulated in over 2/3 of human intrahepatic cholangiocarcinoma PMID: 25229838
    3. analysis of a metal-based inhibitor of NEDD8-activating enzyme PMID: 23185368
    4. our results suggest that APP-BP1 modulates neuronal differentiation by altering gene expression profiles in fetal neural stem cells PMID: 22182960
    5. APP-BP1 plays an important role in the cell cycle progression of fetal neural stem cells PMID: 21151996
    6. APP-BP1 displayed decreased expression levels in HPV-positive head and neck squamous cell carcinoma PMID: 19795456
    7. structure and mutational analysis of human APPBP1-UBA3, the heterodimeric E1 enzyme for NEDD8 PMID: 12646924
    8. Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. PMID: 12740388
    9. Data report the structure of the quaternary complex between human APPBP1-UBA3, a heterodimeric E1, its ubl NEDD8, and ATP. PMID: 14690597
    10. APP-BP1 is degraded by ubiquitin-dependent proteolysis. PMID: 18627766

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  • 亚细胞定位：
    Cell membrane. Note=Colocalizes with APP in lipid rafts.
  • 蛋白家族：
    Ubiquitin-activating E1 family, ULA1 subfamily
  • 组织特异性：
    Ubiquitous in fetal tissues. Expressed throughout the adult brain.
  • 数据库链接：

    HGNC: 621

    OMIM: 603385

    KEGG: hsa:8883

    STRING: 9606.ENSP00000290810

    UniGene: Hs.460978