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MYO5A Antibody

  • 货号：
    CSB-PA857430LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse brain tissue
      All lanes: MYO5A antibody at 7.3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 216, 213, 219 kDa
      Observed band size: 216 kDa
    • IHC image of CSB-PA857430LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA857430LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate
      All lanes: MYO5A antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 216, 213, 219 kDa
      Observed band size: 216 kDa
  • 其他：

产品详情

  • 产品描述：
    MYO5A polyclonal antibody CSB-PA857430LA01HU was produced in the rabbit immunized by using the Recombinant Human Unconventional myosin-Va protein (1087-1220AA) as the immunogen. The target protein MYO5A is processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. This protein is involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane, and may also be required for some polarization process involved in dendrite formation. Diseases associated with MYO5A include Griscelli Syndrome, Type 1 and Griscelli Syndrome, Type 3.
    This Rabbit anti-Homo sapiens (Human) MYO5A Polyclonal antibody was tested in the ELISA, WB, IHC and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It reacts with the MYO5A proteins of human or mouse-origin and may be used to detect the endogenous levels of MYO5A protein.
  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MYO5A Polyclonal antibody
  • Uniprot No.：
    Q9Y4I1
  • 基因名：
    MYO5A
  • 别名：
    non-muscle antibody; Dilute myosin heavy chain antibody; GS1 antibody; MYH12 antibody; MYO5 antibody; Myo5a antibody; MYO5A_HUMAN antibody; Myosin heavy chain 12 antibody; Myosin heavy polypeptide kinase antibody; Myosin V antibody; Myosin VA (heavy polypeptide 12 myoxin) antibody; Myosin VA (heavy polypeptide 12 myoxin) antibody; Myosin-12 antibody; Myoxin antibody; MYR12 antibody; Unconventional myosin-Va antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Unconventional myosin-Va protein (1087-1220AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MYO5A Antibody (CSB-PA857430LA01HU)，的标记方式是Non-conjugated。对于MYO5A Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA857430LB01HU MYO5A Antibody, HRP conjugated ELISA
    FITC CSB-PA857430LC01HU MYO5A Antibody, FITC conjugated
    Biotin CSB-PA857430LD01HU MYO5A Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.
  • 基因功能参考文献：
    1. our studies revealed RPGRIP1L as a novel MyoVa-binding protein - the first to be demonstrated to interact with MyoVa at the centrosome - and uncover an unprecedented link between MyoVa and ciliogenesis, providing new perspectives for studies aiming to better understand why defects in MyoVa cause neurological disorders in Griscelli syndrome patients. PMID: 28266547
    2. MYO5A, having rare amino acid mutations p.R849Q and p.V1601G, was involved in the biological network of known MODY genes. PMID: 29670293
    3. the essential melanocyte-specific transcription factor MITF regulates expression of the MYO5A gene, which encodes the molecular motor myosin-Va. PMID: 27939378
    4. human cytomegalovirus capsids associate with nuclear myosin Va and F-actin and that antagonism of myosin Va impairs capsid localization toward the nuclear rim and nuclear egress. PMID: 29298889
    5. Mechanochemical cycle of myosin-V has been reported. PMID: 28193897
    6. Data suggest that membrane tethering mediated by endosomal RAB11A is drastically and selectively stimulated by its cognate Rab effectors, class V myosins (MYO5A and MYO5B), in a GTP-dependent manner. (RAB11A = ras-related GTPase Rab-11A; MYO5 = myosin class V) PMID: 28939769
    7. ETV6-NTRK3, MYO5A-NTRK3 and MYH9-NTRK3 fusions are identified in Spitz tumours and demonstrated that NTRK3 fusions constitutively activate the mitogen-activated protein kinase, phosphoinositide 3-kinase and phospholipase Cgamma1 pathways in melanocytes. PMID: 27477320
    8. the inhibited Myo5a is equilibrated between the folded state, in which the Mlph-binding site is buried, and the preactivated state, in which the Mlph-binding site is exposed, and that Mlph is able to bind to the Myo5a in preactivated state and activates its motor function. PMID: 27129208
    9. These findings reveal a new fast-acting energy conservation strategy halting growth by immobilizing myosin V in a newly described state on selectively stabilized actin cables. PMID: 25308080
    10. Structural insights into the globular tails of the human type v myosins Myo5a, Myo5b, And Myo5c. PMID: 24339992
    11. Data indicate that myosin Va interacted with multiple new Rab subfamilies including Rab6, Rab14 and Rab39B. PMID: 24006491
    12. several crystal structures of the myosin Va or the myosin Vb globular tail domain that gives insights into how the motor is linked to the recycling membrane compartments via Rab11 or the melanophilin adaptor that binds to Rab27a. PMID: 24248336
    13. the cargo-binding domain (CBD) structures of the three human MyoV paralogs (Va, Vb, and Vc), revealing subtle structural changes that drive functional differentiation and a novel redox mechanism controlling the CBD dimerization process PMID: 24097982
    14. myosin-Va promotes adhesion dynamics, anchorage-independent survival, migration, and invasion in vitro PMID: 23652798
    15. Myosin Va plays a role in the transport and turnover of mRNA. [Review] PMID: 23176491
    16. Calmodulin bound to the first IQ motif is responsible for calcium-dependent regulation of myosin 5a. PMID: 22437832
    17. A Rab27a/MyRIP/myosin Va complex is involved in linking von-Willebrand factor (Vwf) to the peripheral actin cytoskeleton of endothelial cells to allow full maturation and prevent premature secretion of vWF. PMID: 21740491
    18. Myo5a and Rab3A are direct binding partners and interact on synaptic vesicles and the Myo5a/Rab3A complex is involved in transport of neuronal vesicles PMID: 21349835
    19. Myosin Va is required for P body but not stress granule formation. PMID: 21245139
    20. Myo5a is activated in cells during HSV-1 infection to help transport virion- and glycoprotein-laden vesicles from the TGN, through the cortical actin, to the plasma membrane. PMID: 20631136
    21. MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells PMID: 20203291
    22. Myosin-Va has a role in restraining Na(+)/K(+)-ATPase-containing vesicles within intracellular pools. PMID: 19808891
    23. role of function in melanosome transport PMID: 11980908
    24. MYO5A mutations in Griscelli disease PMID: 12148598
    25. Interactions of human Myosin Va isoforms in human melanocytes are tightly regulated by the tail domain. Interaction with rab27a and melanophilin. Myosin Va medial tail domain provides the globular tail domain with organelle-interacting specificity. PMID: 12603861
    26. Griscelli syndrome restricted to hypopigmentation results from a melanophilin defect (GS3) or a MYO5A F-exon deletion (GS1). PMID: 12897212
    27. the endosome-associated protein hrs is a subunit of a protein complex containing actinin-4, BERP, and myosin V that is necessary for efficient TfR recycling but not for EGFR degradation PMID: 15772161
    28. MYO5A transports dense core secretory vesicles in pancreatic MIN6 beta-cells. PMID: 15788565
    29. exon B and its associated dynein light chain have a significant effect on the structure of parts of the coiled-coil tail domains and such a way could influence the regulation and cargo-binding function of myosin Va PMID: 17029413
    30. present a kinetic model for the walking of myosin V on actin PMID: 17487986
    31. Data suggest that myosin-V makes two brownian 90 degrees rotations per 36-nm step as it processively walks on actin filaments in a hand-over-hand fashion. PMID: 17891151
    32. MyoVa directly mediates stable attachment of secretory granules at the plasma membrane. PMID: 17898234
    33. Specific knockdown of MyoVa exon F isoforms resulted in transport inhibition of melanosomes to peripheral subcortical actin network in dendrite tips; perinuclear aggregation of melanosomes. May become innovative drug to treat hyperpigmentation. PMID: 18401430
    34. During primate spermiogenesis, dynein, myosin Va, MyRIP and Rab27b that compose microtubule-based and actin-based vesicle transport systems are actually present in the manchette and might possibly be involved in intramanchette transport. PMID: 18478159
    35. related Rab protein, Rab10, can interact with myosin Va, myosin Vb, and myosin Vc PMID: 19008234
    36. data demonstrate an essential role of myosin Va in cancer cell migration and metastasis, and suggest a novel target for Snail in its regulation of cancer progression PMID: 19521958

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  • 相关疾病：
    Griscelli syndrome 1 (GS1); Griscelli syndrome 3 (GS3); Elejalde syndrome (ELEJAS)
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
  • 组织特异性：
    Detected in melanocytes.
  • 数据库链接：

    HGNC: 7602

    OMIM: 160777

    KEGG: hsa:4644

    STRING: 9606.ENSP00000382177

    UniGene: Hs.21213