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MRGPRX2 Antibody

  • 货号：
    CSB-PA839361LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Positive WB detected in: Hela whole cell lysate, HT29 whole cell lysate, Mouse Brain tissue
      All lanes: MRGPRX2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 38 kDa
      Observed band size: 38 kDa
    • Immunofluorescent analysis of MCF-7 cells using CSB-PA839361LA01HU at dilution of 1:200 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品描述：

    To prepare this MRGPRX2 polyclonal antibody, rabbits were first immunized with the human MRGPRX2 antigen, which stimulates the production of specific antibodies against the MRGPRX2 antigen. The antibodies are then collected and purified using protein G affinity chromatography, resulting in a purity greater than 95%.

    The purified MRGPRX2 polyclonal antibody is then tested in ELISA, WB, and IF. In ELISA, the MRGPRX2 polyclonal antibody can be used as the primary antibody to specifically recognize and bind to the MRGPRX2 antigen from human and mouse samples. This MRGPRX2 antibody can be used to detect and visualize the human and mouse MRGPRX2 proteins in WB and IF, respectively.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MRGPRX2 Polyclonal antibody
  • Uniprot No.：
    Q96LB1
  • 基因名：
  • 别名：
    MRGPRX2; MRGX2; Mas-related G-protein coupled receptor member X2
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Mas-related G-protein coupled receptor member X2 protein (286-330AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MRGPRX2 Antibody (CSB-PA839361LA01HU)，的标记方式是Non-conjugated。对于MRGPRX2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA839361LB01HU MRGPRX2 Antibody, HRP conjugated ELISA
    FITC CSB-PA839361LC01HU MRGPRX2 Antibody, FITC conjugated
    Biotin CSB-PA839361LD01HU MRGPRX2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction. Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.
  • 基因功能参考文献：
    1. Low levels of MRGPRX2 were detected in non-asthma lung mast cells, but its expression was significantly up-regulated in asthma lung mast cells. PMID: 29295703
    2. the present study demonstrates that FceRI and MRGPRX2 function can be inversely regulated by SCF in an acute scenario, suggesting that the signaling requirements differ profoundly between the routes. PMID: 28859248
    3. The s discuss the possibility that MRGPRX2 responds to various as-yet-unidentified endogenous ligands that have specific characteristics, and propose that MRGPRX2 plays an important role in regulating inflammatory responses to endogenous harmful stimuli, such as protein breakdown products released from damaged or dying cells. PMID: 29484687
    4. these peptides are identified as fragments of albumin. The isolated fragments activate MRGPRX2 and degranulate MRGPRX2 expressing LAD 2 cells in dose-dependent manner. The isolated basic peptides generated from human albumin are able to degranulate mast cells via the MRGPRX2. PMID: 28844982
    5. The results showed a kind of mechanism that sinomenine-induced anaphylactoid reactions were mediated by MRGPRX2 via activating PLC molecular signaling pathways to provoke mast cells Ca(2+) mobilization and degranulation. PMID: 28987593
    6. data suggest that mast cells play important roles in both gingival homeostasis and periodontal disease by orchestrating an interaction between gingival epithelial cells and neutrophils via MRGPRX2-mediated degranulation PMID: 28694291
    7. MRGX2 is related to nociception, adrenal gland secretion and mast cell degranulation. (Review) PMID: 26106044
    8. contributes to larger and longer lasting wheal reactions to intradermally injected neuropeptides in patients with chronic urticaria PMID: 24954276
    9. Ectopic expression of MrgX2 in rat basophilic leukemia RBL-2H3 cells and murine bone marrow mast cells renders these cells responsive to human beta-defensin for degranulation. PMID: 23698749
    10. identified MrgX2 as a novel G protein-coupled receptor for the antibacterial peptide LL-37 and demonstrated that unlike most G protein-coupled receptors it is resistant to agonist-induced receptor phosphorylation, desensitization, and internalization. PMID: 22069323
    11. cortistatin-14 shown to be a high affinity ligand for G protein coupled receptor MrgX2 which is expressed in dorsal root ganglion PMID: 12915402
    12. MRGX2 amino acid substitutions may alter the interactions between MRGX2 protein and its ligand, thus, potentially led to adaptive changes of the pain-perception-related nervous system during human evolution. PMID: 15862286

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Mas subfamily
  • 组织特异性：
    Mainly expressed in mast cells. Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion. Detected in blood vessels, scattered lymphocytes, and gastrointestinal ganglia (at protei
  • 数据库链接：

    HGNC: 17983

    OMIM: 607228

    KEGG: hsa:117194

    STRING: 9606.ENSP00000333800

    UniGene: Hs.350566