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MPHOSPH8 Antibody

  • 货号：
    CSB-PA014752GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    Q99549
  • 基因名：
    MPHOSPH8
  • 别名：
    HSMPP8 antibody; M-phase phosphoprotein 8 antibody; M-phase phosphoprotein, mpp antibody; M-phase phosphoprotein, mpp8 antibody; MPHOSPH8 antibody; MPP8_HUMAN antibody; RP11-523H24.1 antibody; Twa3 antibody; Two hybrid-associated protein 3 with RanBPM antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Human MPHOSPH8
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression. Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2. Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene. Mediates down-regulation of CDH1 expression. Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression. The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed.
  • 基因功能参考文献：
    1. The study demonstrated that MPP8 was associated with non-small cell lung cancer cell proliferation through regulation of HOXA5. PMID: 29412790
    2. The haploid screen identified HUSH, an epigenetic heterochromatin repressor complex composed of three subunits, TASOR, MPP8 and Periphilin. (Review) PMID: 26853531
    3. findings demonstrate MPP8 and SIRT1 reciprocally regulate each other's function at multiple molecular layers through physical interaction; disruption of MPP8-SIRT1 interaction de-represses E cadherin expression and reduces cell motility and invasiveness suggesting this interplay plays critical role in MPP8- and SIRT1-mediated epithelial-mesenchymal transition PMID: 25870236
    4. this study identified the HUSH (human silencing hub) complex, comprising three poorly characterized proteins, TASOR, MPP8, and periphilin; this complex is absent from Drosophila but is conserved from fish to humans. PMID: 26022416
    5. Knockdown analysis of MPP8 in HeLa cells resulted in derepression of a set of genes that are normally expressed in spermatogonia, spermatids and mature sperm, thereby indicating a role for this molecule in silencing testis-related genes in somatic cells. PMID: 25660450
    6. interaction of HN with MPP8 may play a role in oncogenesis because MPP8 increases tumor cell motility and invasion PMID: 23532874
    7. these results indicate that the chromatin association of MPP8 is regulated by Cdk-dependent phosphorylation. PMID: 23416073
    8. These findings reveal the molecular mechanism of selective binding of human MPP8 chromodomain to methylated histone H3K9. PMID: 22022377
    9. We determined the crystal structure of MPP8 chromodomain in complex with H3K9me3 peptide. MPP8 interacts with at least six histone H3 residues from glutamine 5 to serine 10. PMID: 21419134
    10. MPP8 mediates E-cadherin gene silencing and promotes breast tumour cell motility and invasion. PMID: 20871592

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 数据库链接：

    HGNC: 29810

    OMIM: 611626

    KEGG: hsa:54737

    STRING: 9606.ENSP00000355388

    UniGene: Hs.269654