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MKLN1 Antibody

  • 货号：
    CSB-PA890926LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Rat brain tissue
      All lanes: MKLN1 antibody at 2.7µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 85, 61 kDa
      Observed band size: 85 kDa
    • IHC image of CSB-PA890926LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA890926LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MKLN1 Polyclonal antibody
  • Uniprot No.：
    Q9UL63
  • 基因名：
    MKLN1
  • 别名：
    MKLN 1 antibody; Mkln1 antibody; MKLN1_HUMAN antibody; muskelin 1, intracellular mediator containing kelch motifs antibody; Muskelin antibody; TWA2 antibody; Two-hybrid-associated protein with Ranbpm 2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human Muskelin protein (488-614AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MKLN1 Antibody (CSB-PA890926LA01HU)，的标记方式是Non-conjugated。对于MKLN1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA890926LB01HU MKLN1 Antibody, HRP conjugated ELISA
    FITC CSB-PA890926LC01HU MKLN1 Antibody, FITC conjugated
    Biotin CSB-PA890926LD01HU MKLN1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Required for internalization of the GABA receptor GABRA1 from the cell membrane via endosomes and subsequent GABRA1 degradation. Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1.
  • 基因功能参考文献：
    1. A meta-analysis genome wide association study suggest MKLN1 is associated with early-onset bipolar disorder. PMID: 27769005
    2. RanBPM, ARMC8alpha, ARMC8beta, Muskelin, p48EMLP, and p44CTLH form complexes in cells PMID: 17467196
    3. These novel findings identify a role for muskelin-RanBP9 complex in pathways that integrate cell morphology regulation and nucleocytoplasmic communication. PMID: 18710924
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytosol. Nucleus, nucleoplasm. Cell projection, ruffle. Cytoplasm, cell cortex. Cell junction, synapse. Cell junction, synapse, postsynapse.
  • 数据库链接：

    HGNC: 7109

    OMIM: 605623

    KEGG: hsa:4289

    STRING: 9606.ENSP00000323527

    UniGene: Hs.44693