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METAP2 Antibody

  • 货号：
    CSB-PA013716LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Rat liver tissue
      All lanes: METAP2 antibody at 3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 53, 52, 51 kDa
      Observed band size: 53 kDa
    • IHC image of CSB-PA013716LA01HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA013716LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) METAP2 Polyclonal antibody
  • Uniprot No.：
    P50579
  • 基因名：
  • 别名：
    A930035J23Rik antibody; AI047573 antibody; AL024412 antibody; Amp2 antibody; AU014659; antibody; EIF 2 associated p67 homolog antibody; EIF-2-associated p67 antibody; Initiation factor 2 associated 67 kDa glycoprotein antibody; Initiation factor 2-associated 67 kDa glycoprotein antibody; MAP 2 antibody; MAP2 antibody; MAP2_HUMAN antibody; MetAP 2 antibody; Metap2 antibody; Methionine aminopeptidase 2 antibody; Methionyl aminopeptidase 2 antibody; MGC102452 antibody; MGC127390 antibody; MGC53792 antibody; MNPEP antibody; p67 antibody; p67eIF2 antibody; Peptidase M antibody; Peptidase M2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human Methionine aminopeptidase 2 protein (313-449AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，METAP2 Antibody (CSB-PA013716LA01HU)，的标记方式是Non-conjugated。对于METAP2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA013716LB01HU METAP2 Antibody, HRP conjugated ELISA
    FITC CSB-PA013716LC01HU METAP2 Antibody, FITC conjugated
    Biotin CSB-PA013716LD01HU METAP2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
  • 基因功能参考文献：
    1. MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
    2. Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
    3. Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
    4. Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
    5. The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
    6. Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
    7. This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
    8. the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
    9. Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
    10. the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
    11. High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
    12. Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
    13. MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
    14. Expressed in colon cancer cells PMID: 15336565
    15. MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
    16. A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
    17. Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
    18. MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
    19. Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
    20. Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176

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  • 亚细胞定位：
    Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.
  • 蛋白家族：
    Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily
  • 数据库链接：

    HGNC: 16672

    OMIM: 601870

    KEGG: hsa:10988

    STRING: 9606.ENSP00000325312

    UniGene: Hs.444986