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MELTF Antibody

  • 货号：
    CSB-PA013754LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate
      All lanes: MELTF antibody at 3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 81, 33 kDa
      Observed band size: 81 kDa
    • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA013754LA01HU at dilution of 1:100
    • Immunofluorescent analysis of HepG2 cells using CSB-PA013754LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品描述：

    The polyclonal antibody CSB-PA013754LA01HU especially targets the MELTF protein. To create this antibody, a rabbit was inoculated with recombinant human Melanotransferrin protein (20-302aa), and protein G affinity chromatography was employed to purify the antibody. The purity of this MELTF polyclonal antibody is greater than 95%.

    The MELTF polyclonal antibody can detect MELTF protein in both human and mouse samples. Multiple rigorous validations have confirmed its efficacy in various applications, including ELISA, WB, IHC, and IF. Therefore, researchers can utilize this antibody to quantify MELTF protein levels, identify its presence and size, and localize the MELTF protein.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MELTF Polyclonal antibody
  • Uniprot No.：
    P08582
  • 基因名：
  • 别名：
    Antigen p97 antibody; CD228 antibody; CD228 antigen antibody; MAP97 antibody; Melanoma associated antigen p97 antibody; Melanoma-associated antigen p97 antibody; Melanotransferrin antibody; Mfi2 antibody; MTF1 antibody; TRFM_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Melanotransferrin protein (20-302AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MELTF Antibody (CSB-PA013754LA01HU)，的标记方式是Non-conjugated。对于MELTF Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA013754LB01HU MELTF Antibody, HRP conjugated ELISA
    FITC CSB-PA013754LC01HU MELTF Antibody, FITC conjugated
    Biotin CSB-PA013754LD01HU MELTF Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
  • 基因功能参考文献：
    1. Melanotransferrin is a serological marker of colorectal cancer by secretome analysis and quantitative proteomics. PMID: 25216327
    2. PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low. PMID: 21190562
    3. The human genome contains a second melanotransferrin gene (MTf2). There are two isoforms of melanotransferrin. PMID: 11852110
    4. its ability to donate 59Fe to SK-Mel-28 melanoma cells and other cell types PMID: 12230555
    5. membrane-bound and soluble p97 affect the migration capacity of endothelial and melanoma cells and suggest that p97 could be involved in the regulation of plasminogen activation by interacting with pro-uPA and plasminogen. PMID: 12750156
    6. Serum melanotransferrin exists in various conformations depending on the binding of bivalent cations or following post-translational modification; serum melanotransferrin levels are unchanged in subjects with Alzheimer's disease. PMID: 12809550
    7. The distribution of MTf and its splice variants may provide clues to their possible biological roles in melanoma and other tumors. PMID: 15716025
    8. melanotransferrin has a role in cell proliferation and tumorigenesis PMID: 16704991
    9. Anti-angiogenic properties of soluble melanotransferrin may result from local overstimulation of plasminogen activation by tissue plasminogen activator, thus leading to subsequent degradation of the fibronectin matrix and endothelial cell detachment. PMID: 16713448
    10. Altogether, these findings strongly suggest that MTf overexpression in melanoma cells contributes to tumor progression by stimulating plasmin generation as well as cell migration and invasion. PMID: 17196552
    11. HMB-45 (melanoma-associated antigen p97) and Melan-A combined were positive in 100% of the renal angiomyolipomas PMID: 17227112
    12. Novel molecular targets directly or indirectly regulated by MTf and the potential pathways involved in its function, including modulation of proliferation. PMID: 17449903
    13. this review discusses the studies that have demonstrated that melanotransferrin is not involved in iron metabolism, but plays a vital role in melanoma cell proliferation and tumorigenesis. PMID: 17452986
    14. Biochemical and spectroscopic studies of MTF1 are reported. PMID: 18691669

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Transferrin family
  • 组织特异性：
    Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.
  • 数据库链接：

    HGNC: 7037

    OMIM: 155750

    KEGG: hsa:4241

    STRING: 9606.ENSP00000296350

    UniGene: Hs.184727