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MED25 Antibody

  • 货号：
    CSB-PA741144HA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA741144HA01HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MED25 Polyclonal antibody
  • Uniprot No.：
    Q71SY5
  • 基因名：
    MED25
  • 别名：
    ACID1 antibody; Activator interaction domain 1 protein antibody; Activator interaction domain-containing protein 1 antibody; Activator-recruited cofactor 92 kDa component antibody; ARC/mediator transcriptional coactivator subunit antibody; ARC/mediator transcriptional coactivator subunit ARC92 antibody; ARC92 antibody; BVSYS antibody; CMT2B2 antibody; DKFZp434K0512 antibody; FLJ10193 antibody; med25 antibody; MED25 protein antibody; MED25_HUMAN antibody; Mediator complex subunit 25 antibody; Mediator of RNA polymerase II transcription subunit 25 antibody; Mediator of RNA polymerase II transcription; subunit 25 homolog (S cerevisiae) antibody; Mediator of RNA polymerase II transcription; subunit 25 homolog (yeast) antibody; MGC70671 antibody; p78 antibody; prostate-derived protein 78 kDa antibody; PTOV2 antibody; TCBAP0758 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Mediator of RNA polymerase II transcription subunit 25 protein (112-140AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MED25 Antibody (CSB-PA741144HA01HU)，的标记方式是Non-conjugated。对于MED25 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA741144HB01HU MED25 Antibody, HRP conjugated ELISA
    FITC CSB-PA741144HC01HU MED25 Antibody, FITC conjugated
    Biotin CSB-PA741144HD01HU MED25 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
  • 基因功能参考文献：
    1. FOS strongly binds to the same MED25 site as ETV4 activation domain and JUN interacts with the other two MED25 sites. PMID: 28728983
    2. Med25, a variable member of the Mediator complex, is a coactivator of ligand-activated ERalpha that interacts with ERalpha through its C-terminal LXXLL motif after BPA exposure, and is functionally involved in BPA-induced transcriptional regulation of CYP2C9 expression and enzyme activity. PMID: 27273787
    3. the critical region of chromosome 19 a homozygous variant (c.418C>T, p.Arg140Trp) in Mediator complex subunit 25 predicted as deleterious. PMID: 25527630
    4. A model is proposed in which the N-terminal transactivation domain of ERM binds the MED25 VP16 H1 binding pocket in a helical conformation. PMID: 26130716
    5. Homozygous MED25 mutation implicated in eye-intellectual disability syndrome. PMID: 25792360
    6. MED25 is important for regulating the epigenetic landscape resulting in transcriptional activation of a highly inducible gene, CYP2C9 PMID: 25391650
    7. Med25 is identified as a new coactivator of ERalpha that is required for ERalpha-mediated regulation of CYP2C9 expression PMID: 24960263
    8. Mediator subunit MED25 plays a critical role in this process and identify a MED25 domain that serves as a docking site on Mediator for the ATF6alpha transcription activation domain. PMID: 23864652
    9. the identification of MED25 as one of the HNF4alpha binding partners in pancreatic ss-cells leading to insulin secretion which is impaired in MODY patients PMID: 22952853
    10. solution NMR structure of protein MED25(391-543), comprising the activator interacting domain of subunit 25 of mediator, is presented with the measurement of polypeptide backbone heteronuclear 15N-{1H} NOEs to identify fast internal motional modes PMID: 21785987
    11. The regulatory interaction between Sox9, Wwp2 and Med25 defines the Sox9 transcriptional mechanisms of chondrogenesis in the forming palate. PMID: 21427722
    12. Preliminary NMR chemical shift mapping showed that VP16 H2 (VP16C) interacts with MED25 ACID through one face of the beta-barrel, defined by strands B4-B7-B6. PMID: 20974256
    13. A hydrophobic furrow, formed by a beta-barrel and two alpha-helices in MED25 VBD, interacts tightly with VP16 TADn PMID: 21378963
    14. report the NMR structure of the Mediator subunit Med25 (also called Arc92) activator interaction domain (ACID) and analyze the structural and functional interaction of ACID with the archetypical acidic transcription activator VP16 PMID: 21378965
    15. These results define a role for Med25 and the Mediator complex in the regulation of xenobiotic metabolism and lipid homeostasis. PMID: 21135126
    16. Lana-1-induced serum response element activation was inhibited by the dominant-negative N-terminal domain of the human MED25 mediator subunit, suggesting that this subunit mediates Lana-1-induced serum response element activation. PMID: 20089804
    17. cloned a prostate-derived cDNA encoding a novel protein with a predicted molecular weight of 78 kDa (P(78)), and precisely mapped the cDNA sequence to chromosome 19 PMID: 12163014
    18. biochemical purification and cloning of ARC92; identified as a specific cellular interaction partner of the VP16 activation domain PMID: 14983011
    19. data suggest that MED25, in cooperation with CBP and Mediators through its distinct domains, imposes a selective advantage on RAR/RXR activation PMID: 17641689
    20. Data show that Med25 is coordinately expressed with Pmp22 gene dosage and expression in transgenic mice and rats, and suggest a potential role of this protein in the molecular etiology of Charcot-Marie-Tooth disease. PMID: 19290556

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  • 相关疾病：
    Charcot-Marie-Tooth disease 2B2 (CMT2B2); Basel-Vanagaite-Smirin-Yosef syndrome (BVSYS)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Mediator complex subunit 25 family
  • 组织特异性：
    Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.
  • 数据库链接：

    HGNC: 28845

    OMIM: 605589

    KEGG: hsa:81857

    STRING: 9606.ENSP00000326767

    UniGene: Hs.656639