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MADD Antibody

  • 货号：
    CSB-PA003185
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of Colo205 cells using MADD Polyclonal Antibody
    • Western Blot analysis of LOVO cells using MADD Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q8WXG6
  • 基因名：
    MADD
  • 别名：
    Differentially expressed in normal and neoplastic cells antibody; FLJ35600 antibody; IG20 antibody; Insulinoma glucagonoma clone 20 antibody; KIAA0358 antibody; MADD antibody; MADD_HUMAN antibody; MAP kinase-activating death domain protein antibody; Rab3 GDP/GTP exchange factor antibody; RAB3GEP antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human MADD.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family. Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion. Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction. Involved in upregulating a post-docking step of synaptic exocytosis in central synapses. Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals. Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2. May link TNFRSF1A with MAP kinase activation. May be involved in the regulation of TNFA-induced apoptosis.
  • 基因功能参考文献：
    1. The risk of coronary disease and ischemic stroke associated with multiple polymorphisms and haplotypes of MADD and FOLH1 in Han Chinese patients are reported in association with alcohol consumption. PMID: 27070640
    2. miR-3151 silencing by DNA methylation protected chronic lymphocytic leukemia cells from apoptosis by over-expression of its direct targets MADD and PIK3R2, constitutive activation of MEK/ERK and PI3K/AKT signaling , and over-expression of MCL1. PMID: 26517243
    3. Taken together, our data reveal that PTEN can convey the death signal by preventing MADD phosphorylation by Akt. PMID: 24038283
    4. These results suggest that MADD may be a potential therapeutic target for lung adenocarcinoma therapy involving the TRAIL-induced apoptosis pathway PMID: 23443411
    5. MADD knockdown resulted in enhanced spontaneous apoptosis in human breast cancer cell lines PMID: 23457619
    6. We analyzed DENN/MADD/IG20 (DMI), the complex of four splice variants in Alzheimer's disease. PMID: 22678883
    7. SNP rs2290149, located in a genetic cluster of MYBPC3 and MADD gene, was found to be associated with diastolic heart failure. PMID: 22529996
    8. Data show that ehe expression of MAPK-activating death domain protein (MADD) increases obviously in lung adenocarcinoma, and MADD can promote survival of lung adenocarcinoma cells by inhibiting apoptosis. PMID: 22482404
    9. The SNP in ADCY5 is implicated in defective proinsulin-to-insulin conversion and previous findings on the role of a genetic variant in MADD on proinsulin-to-insulin conversion, were confirmed. PMID: 21887289
    10. Data show that SNPs from MADD, PROX1, and SLC30A8 were associated with type 2 diabetes. PMID: 21103350
    11. Endogenous MADD is phosphorylated at three highly conserved sites by Akt, and only the phosphorylated MADD can directly interact with the TRAIL receptor DR4 thereby preventing Fas-associated death domain recruitment. PMID: 20484047
    12. Denn is intimately involved in anti-apoptotic and cell survival processes in cancer cells PMID: 12410563
    13. IG20 overexpression enhanced the combined apoptotic effects of TRAIL and gamma-irradiation on HeLa cells. PMID: 14695193
    14. IG20 suppresses tumor cell survival and enhances susceptibility to apoptosis and cancer therapy. PMID: 14716293
    15. DENN has anti-apoptotic and cell survival roles in tumor cells PMID: 14735464
    16. IG20 (MADD splice variant-5) enhances TRAIL-induced apoptosis by increasing recruitment of FADD and caspase-8 to the death-inducing signaling complex. PMID: 15208670
    17. Knockdown of MADD can render cells susceptible to spontaneous apoptosis but had no discernible effect on cell proliferation, colony size or cell cycle progression. MADD alone is sufficient and necessary for cancer cell survival. PMID: 16682944
    18. MADD has a role in the control of cancer cell survival/death as a negative regulator of caspase-8 and confers significant resistance to TRAIL-induced apoptosis PMID: 17314102
    19. data strongly indicate that germline mutations in SPI1 and MADD genes do not confer a high risk of chronic lymphocytic leukaemia and do not make a major contribution to the familial risk of the disease PMID: 17410194
    20. we have identified an essential role for splice variants of the IG20 gene in TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)resistance. PMID: 18223207
    21. Expression of KIAA0358 exerted a potent antiapoptotic effect in both the SK-N-SH and SH-SY5Y neuroblastoma cell lines, whereas expression of IG20-SV4 had proapoptotic effects directly related to the activation of caspase-8 in these cells. PMID: 18794122
    22. KIF1Bbeta- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through MADD. PMID: 18849981
    23. Loss of MADD expression resulted in reduced Grb2 and Sos1/2 recruitment to the TNFR1 complex and decreased Ras and MEKK1/2 activation PMID: 19289468

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  • 亚细胞定位：
    Cell membrane. Cytoplasm. Cell projection, axon.
  • 蛋白家族：
    MADD family
  • 组织特异性：
    Expressed in testis, ovary, brain and heart. Expressed in spleen, thymus, prostate, testis, ovary, small instestine and colon. Expressed in liver.; [Isoform 1]: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and
  • 数据库链接：

    HGNC: 6766

    OMIM: 603584

    KEGG: hsa:8567

    STRING: 9606.ENSP00000310933

    UniGene: Hs.82548