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MAD2L1BP Antibody

  • 货号：
    CSB-PA246770
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA246770(MAD2L1BP Antibody) at dilution 1/65, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA246770(MAD2L1BP Antibody) at dilution 1/65, on the right is treated with fusion protein. (Original magnification: ×200)
    • Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-4: A549 cells, 231 cells, PC3 cells, hela cells, Primary antibody: CSB-PA246770(MAD2L1BP Antibody) at dilution 1/675, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
  • 其他：

产品详情

  • Uniprot No.：
    Q15013
  • 基因名：
    MAD2L1BP
  • 别名：
    Caught by MAD2 protein antibody; CMT2 antibody; dJ261G23.1 antibody; KIAA0110 antibody; MAD2L1 binding protein antibody; MAD2L1 binding protein isoform 2 antibody; MAD2L1-binding protein antibody; Mad2l1bp antibody; MD2BP_HUMAN antibody; MGC11282 antibody; OTTHUMP00000016496 antibody; RP1 261G23.6 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Fusion protein of Human MAD2L1BP
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    WB 1:500-1:2000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
  • 基因功能参考文献：
    1. MAD2-p31(comet) axis may serve as a potential therapeutic target for glioma. PMID: 29408509
    2. p31comet-induced cell death is mediated by interactions with Mad2 PMID: 26544187
    3. joint action of TRIP13 and p31(comet) also promotes the release of Mad2 from MCC, participates in the complete disassembly of MCC and abrogates checkpoint inhibition of APC/C PMID: 25092294
    4. Altered p31(Comet):Mad2 expression ratios may provide new insight into altered spindle assembly checkpoint function and the generation of chromosomal instability in tumors. PMID: 24131926
    5. attenuating the affinity of p31(Comet) for Mad2 by phosphorylation promotes SAC activity in mitosis. Specifically, phosphorylation of Ser-102 weakens p31(Comet)-Mad2 binding and enhances p31(Comet)-mediated bypass of the SAC. PMID: 24596092
    6. study found p31(comet) traffics on and off kinetochores and also present in the cytosol; Cells depleted of p31(comet) arrest in metaphase with mature bipolar kinetochore-microtubule attachments; propose that p31(comet) is an essential component of machinery that silences the checkpoint during each cell cycle PMID: 21965286
    7. the importance of p31(comet) in checkpoint silencing and its potential as a target for antimitotic therapies. PMID: 22544748
    8. The binding of p31(comet) to Mad2 in mitotic checkpoint complex may trigger a conformational change in Cdc20 that facilitates its phosphorylation by Cdk, and that the latter process may promote its dissociation from BubR1. PMID: 22566641
    9. both p31(comet) and ubiquitination of Cdc20 are critical mechanisms of checkpoint inactivation. They act redundantly to promote Mad2 dissociation from Cdc20 PMID: 21937719
    10. p31(comet) negatively regulates the spindle assembly checkpoint by extracting Mad2 from the MCC. PMID: 22100920
    11. This review presents knowledge of CMT2 and possible pathogenetic mechanisms responsible for the disease PMID: 22235654
    12. p31comet localizes to kinetochores in a C-Mad2-dependent manner. the Mad1:C-Mad2 complex undergoes regulation by p31comet-dependent 'capping'. PMID: 21772247
    13. Although p31(comet) binds to Mad2, it promotes the dissociation of Cdc20 from BubR1 in MCC. PMID: 21300909
    14. p31(comet, formerly known as Cmt2) counteracts the function of Mad2 and is required for the silencing of the spindle checkpoint PMID: 15257285
    15. p31(comet) does not have an impact on the formation of aneuploidy and tetraploidy found in human hepatocellular carcinoma. PMID: 17934339
    16. Data suggest that p31(comet)(CMT2) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2." PMID: 18022368
    17. p31(comet) induces tumor cell senescence by mediating p21(Waf1/Cip1) accumulation and Mad2 disruption and that these effects are dependent on a direct interaction of p31(comet) with Mad2. PMID: 19276188

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Note=During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
  • 蛋白家族：
    MAD2L1BP family
  • 数据库链接：

    HGNC: 21059

    KEGG: hsa:9587

    STRING: 9606.ENSP00000410818

    UniGene: Hs.122346