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MACF1 Antibody

  • 货号：
    CSB-PA013299GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    Q9UPN3
  • 基因名：
  • 别名：
    MACF1 antibody; ABP620 antibody; ACF7 antibody; KIAA0465 antibody; KIAA1251 antibody; Microtubule-actin cross-linking factor 1 antibody; isoforms 1/2/3/5 antibody; 620 kDa actin-binding protein antibody; ABP620 antibody; Actin cross-linking family protein 7 antibody; Macrophin-1 antibody; Trabeculin-alpha antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Human MACF1
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    F-actin-binding protein which plays a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane. Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics. Interaction with CAMSAP3 at the minus ends of non-centrosomal microtubules tethers microtubules minus-ends to actin filaments, regulating focal adhesion size and cell migration. May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4. Plays a key role in wound healing and epidermal cell migration. Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate microtubule dynamics and polarize hair follicle stem cells. As a regulator of actin and microtubule arrangement and stabilization, it plays an essential role in neurite outgrowth, branching and spine formation during brain development.
  • 基因功能参考文献：
    1. Loss of ACF7 leads to aberrant microtubule organization, tight junction stabilization and impaired wound closure in vitro. ACF7 level is also correlated with development and progression of ulcerative colitis (UC) in patients. PMID: 28541346
    2. Study summarized the physiological role of MACF1 as well as its pathological one in various cancers. MACF1 comprises different isoforms, and is broadly expressed in brain, spinal cord, lung, kidney, heart, bone and skeletal muscles tissues. It plays a crucial role in cell proliferation, migration and cell signaling, and is also closely associated with many cancer. PMID: 28782898
    3. in mammalian intestinal epithelial cells, the spectraplakin ACF7 (also known as MACF1) specifically binds to CAMSAP3 and is required for the apical localization of CAMSAP3-decorated microtubule minus ends. PMID: 27802168
    4. MACF1b may contribute to the genetic etiology and mechanistic causation of Parkinson's disease. PMID: 27021023
    5. ACF7, a member of the spectraplakin family of cytoskeletal crosslinking proteins, interacts with Nezha (also called CAMSAP3) at the minus ends of noncentrosomal microtubules and anchors them to actin filaments. PMID: 27693509
    6. the present study represents the first investigation on the functional role of MACF1 in tumor cell biology, as well as demonstrates its potential as a unique biomarker that can be targeted synergistically with TMZ as part of a combinatorial therapeutic approach for the treatment of genetically multifarious glioblastomas PMID: 27959385
    7. Duplication in the microtubule-actin cross-linking factor 1 gene causes neuromuscular diseases. PMID: 24899269
    8. uncovered a role for ELMO in the recruitment of ACF7 to the membrane to promote microtubule capture and stability PMID: 23184944
    9. ACF7 targeting to the plasma membrane is both required and sufficient for microtubule capture downstream of ErbB2. PMID: 20937854
    10. p230, through its interaction with MACF1, provides the molecular link for transport of GPI-anchored proteins along the microtubule and actin cytoskeleton from the TGN to the cell periphery. PMID: 15265687
    11. In two lung cell lines, MACF1b was chiefly localized to the Golgi complex. The domain of MACF1b that targets it to the Golgi was found at the N-terminal part of the region that contains the plakin repeats. PMID: 16076900

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  • 亚细胞定位：
    [Isoform 2]: Cytoplasm, cytoskeleton. Cytoplasm. Golgi apparatus. Cell membrane. Cell projection, ruffle membrane.; [Isoform 1]: Cytoplasm. Golgi apparatus.
  • 蛋白家族：
    Plakin or cytolinker family
  • 组织特异性：
    Isoform 2: Ubiquitously expressed. Isoform 1: Expressed in cell lines NCI-H460, A-549 and HaCaT. Isoform 4: Expressed in heart, lung, pituitary and placenta, not found in brain, kidney, liver, pancreas or skeletal muscle.
  • 数据库链接：

    HGNC: 13664

    OMIM: 608271

    KEGG: hsa:23499

    STRING: 9606.ENSP00000354573

    UniGene: Hs.472475