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LIG3 Antibody

  • 货号：
    CSB-PA012931LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA012931LA01HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA012931LA01HU at dilution of 1:100
    • Immunofluorescent analysis of Hela cells using CSB-PA012931LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) LIG3 Polyclonal antibody
  • Uniprot No.：
    P49916
  • 基因名：
  • 别名：
    D11Wsu78e antibody; DNA ligase antibody; DNA ligase 3 antibody; DNA ligase III antibody; DNLI3_HUMAN antibody; LIG 3 antibody; LIG2 antibody; LIG3 antibody; Ligase II; DNA; ATP dependent antibody; Ligase III DNA ATP dependent antibody; MGC78176 antibody; OTTMUSP00000000875 antibody; Polydeoxyribonucleotide synthase [ATP] 3 antibody; Polydeoxyribonucleotide synthase [ATP] antibody; Polydeoxyribonucleotide synthase antibody; RP23-316C11.6 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human DNA ligase 3 protein (809-928AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，LIG3 Antibody (CSB-PA012931LA01HU)，的标记方式是Non-conjugated。对于LIG3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA012931LB01HU LIG3 Antibody, HRP conjugated ELISA
    FITC CSB-PA012931LC01HU LIG3 Antibody, FITC conjugated
    Biotin CSB-PA012931LD01HU LIG3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair.
  • 基因功能参考文献：
    1. The homozygotes of rs1052536 TT were associated with an increased risk for NTDs than CC, and variants of rs1052536 T were associated with an increased risk of Neural Tube Defects. The stratified analysis showed that TT genotype of rs1052536 increased the risk of anencephaly and the T allele significantly increased the risk of cranial Neural Tube Defects. PMID: 30022792
    2. single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase I PMID: 28751376
    3. The g.29661G>A and g.29059C>T polymorphisms of LIG3 may play a role in the keratoconus and Fuchs endothelial corneal dystrophy pathogenesis and can be considered as markers in these diseases. PMID: 25817347
    4. A computational approach to determine susceptibility to cancer by evaluating the deleterious effect of nsSNP in XRCC1 gene on binding interaction of XRCC1 protein with ligase III. PMID: 26449312
    5. In the context of tyrosine kinase-activated leukemias, c-MYC contributes to aberrant DNA repair through downstream targets LIG3 and PARP1 up-regulation. PMID: 25828893
    6. Domains constituting the LigIII catalytic core collaborate and are essential for formation of a DNA-bridging intermediate by adenylated LigIII that positions a pair of blunt-ended duplex DNAs for efficient and specific intermolecular ligation. PMID: 26130724
    7. there is an absolute requirement for fully functional DNA ligase III (LIG3), but not ligase IV (LIG4), to facilitate the escape from a telomere-driven crisis. PMID: 25127141
    8. LIGIII plays a role in additional EJing repair pathway only in the absence of Ku. PMID: 24837021
    9. Results show that overexpression of DNA ligase III in mitochondria improves mitochondrial base excision repair and enhances cell survival after oxidative stress. PMID: 24674627
    10. data confirm previous work showing that Lig3 is required to maintain mtDNA integrity and function, and highlight a new function of ATM in regulating DNA Lig3 stability and consequently mtDNA repair PMID: 24342190
    11. Human Mre11/human Rad50/Nbs1 and DNA ligase IIIalpha/XRCC1 protein complexes act together in an alternative nonhomologous end joining pathway. PMID: 21816818
    12. Using our cohort of 480 breast cancer patients, we provide replicated evidence that a polymorphism near the LIG3 gene is associated with acute skin toxicity following radiotherapy. PMID: 21620500
    13. results establish a role for Lig3 in mitochondria, but distinguish it from its interacting protein Xrcc1 PMID: 21390132
    14. The collective results support a "jackknife model" in which the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. PMID: 20518483
    15. The interaction with Lig3alpha is promoted by serine 81 that is located within a putative S/TQ site in the N-terminus domain of TDP1. PMID: 20009512
    16. calpain-mediated proteolysis of DNA ligase III plays an essential role in DNA damage-induced cell death in human cells PMID: 11994275
    17. In response to DNA damage, DNA ligase IIIalpha translocate from centrosomes to chromosomes. PMID: 15653642
    18. XRCC1 stimulates Pol beta strand displacement activity and releases inhibition of Pol beta by DNA-bound Lig III if ligation is prevented. PMID: 16442856
    19. the interaction of DNA ligase III and DNA polymerase gamma is required for proper maintenance of the mammalian mitochondrial genome. PMID: 17054425
    20. Results describe the activity requirements for DNA ligases III and IV in the pathways of non-homologous DNA end joining. PMID: 17492771
    21. key role for XRCC1-DNA ligase IIIalpha in maintenance of genomic integrity by nucleotide excision repair in both dividing and nondividing cells PMID: 17643379
    22. analysis of DNA-binding and nick recognition modules in human DNA ligase III PMID: 18238776
    23. Data show that CHIP-mediated degradation and DNA damage-dependent stabilization regulate base excision repair proteins XRCC1, DNA polymerase beta, and DNA ligase III. PMID: 18313385
    24. siRNA mediated down-regulation of DNA ligase III in human HTD114 cells led to impaired end joining that was mediated by 2-, 3- or 10-bp microhomology. PMID: 18440984
    25. altered DNA double-strand break repair in CML cells is caused by the increased activity of an alternative nonhomologous end-joining repair pathway, involving DNA ligase IIIalpha and WRN PMID: 18524993
    26. PMID 10207110 showed that translation initiation at the first ATG in the cDNA can lead to a mitochondrially-targeted isoform of the protein. PMID: 10207110

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  • 亚细胞定位：
    [Isoform 1]: Mitochondrion.; [Isoform 2]: Mitochondrion.; [Isoform 3]: Nucleus.; [Isoform 4]: Nucleus.
  • 蛋白家族：
    ATP-dependent DNA ligase family
  • 组织特异性：
    Testis, thymus, prostate and heart.
  • 数据库链接：

    HGNC: 6600

    OMIM: 600940

    KEGG: hsa:3980

    STRING: 9606.ENSP00000367787

    UniGene: Hs.100299