Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. KRT12 Antibody

KRT12 Antibody

  • 货号：
    CSB-PA859510LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunofluorescence staining of Hela cells with CSB-PA859510LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) KRT12 Polyclonal antibody
  • Uniprot No.：
    Q99456
  • 基因名：
  • 别名：
    CK-12 antibody; Cytokeratin-12 antibody; K12 antibody; K1C12_HUMAN antibody; Keratin 12 antibody; Keratin antibody; Keratin; type I cytoskeletal 12 antibody; Keratin-12 antibody; KRT12 antibody; type I cytoskeletal 12 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Keratin, type I cytoskeletal 12 protein (1-178AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，KRT12 Antibody (CSB-PA859510LA01HU)，的标记方式是Non-conjugated。对于KRT12 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA859510LB01HU KRT12 Antibody, HRP conjugated ELISA
    FITC CSB-PA859510LC01HU KRT12 Antibody, FITC conjugated
    Biotin CSB-PA859510LD01HU KRT12 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in corneal epithelium organization, integrity and corneal keratin expression.
  • 基因功能参考文献：
    1. KC can co-exist with GCD. The missense mutation (c.370G > A) in the TGFBI gene and insert mutation (c.1456-1457ins GAT) in the KRT12 gene were identified in a 23-year-old male patient with concurrent KC and GCD. PMID: 28567551
    2. Combined with an effective delivery vehicle this siRNA approach represents a viable treatment option for prevention of the MECD pathology observed in K12-Leu132Pro heterozygous individuals. PMID: 24801514
    3. We identified a novel missense mutation of the KRT12 gene in Meesmann corneal dystrophy. The in vivo confocal microscopy examinations revealed previously unreported depth-dependent ultrastructural changes in the living cornea. PMID: 24099278
    4. The Leu132Pro missense mutation is within the helix-initiation motif of the keratin and is predicted to result in a significant structural change of the K12 protein. PMID: 23222558
    5. The lead siRNA, with an IC(50) of thirty picomolar, showed no keratin off-target effects or activation of TLR3 in the concentration ranges tested. PMID: 23233254
    6. Novel missense mutation within the highly conserved helix-initiation motif of KRT12 causing Meesmann's corneal dystrophy in a German family. PMID: 20577595
    7. Heterozygous Ala137Pro mutation in keratin 12 gene found in Japanese with Meesmann's corneal dystrophy. PMID: 12543196
    8. A novel missense mutation (Y429C) in KRT12 lead to MCD in 2 unrelated Taiwanese families. PMID: 16227835
    9. Mutation in the KRT12 gene is associated with Meesmann corneal dystrophy PMID: 16352477
    10. Mutation associated with symptomatic phenotype of Meesmann's corneal dystrophy. Results in substitution of proline for arginine in helix termination motif that may disrupt normal helix, leading to dramatic structural change of keratin 12 protein. PMID: 17653038
    11. This is the second family recently diagnosed with Meesmann dystrophy in Denmark. The family represents its own distinct genotype, independent of previously reported ones. All patients with microcysts were asymptomatic. PMID: 18245975
    12. The novel L433R mutation of the KRT12 gene found in two members of this Japanese family caused Meesmann corneal dystrophy (MECD). PMID: 18661274

    显示更多

    收起更多

  • 相关疾病：
    Corneal dystrophy, Meesmann (MECD)
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in the corneal epithelium (at protein level).
  • 数据库链接：

    HGNC: 6414

    OMIM: 122100

    KEGG: hsa:3859

    STRING: 9606.ENSP00000251643

    UniGene: Hs.66739