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KIF23 Antibody

  • 货号：
    CSB-PA23569A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA23569A0Rb diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品描述：
    KIF23 polyclonal antibody CSB-PA23569A0Rb was produced in the rabbit immunized by using the Recombinant Human Kinesin-like protein KIF23 protein (865-960AA) as the immunogen. The target protein KIF23, a member of kinesin-like protein family, is component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. It is essential for cytokinesis in Rho-mediated signaling and required for the localization of ECT2 to the central spindle. Diseases associated with KIF23 include Anemia, Congenital Dyserythropoietic, Type Iii and Congenital Dyserythropoietic Anemia.
    This Rabbit anti-Homo sapiens (Human) KIF23 Polyclonal antibody was tested in the ELISA and IHC applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the KIF23 proteins of human-origin and may be used to detect the endogenous levels of KIF23 protein.
  • 产品名称：
    Rabbit anti-Homo sapiens (Human) KIF23 Polyclonal antibody
  • Uniprot No.：
    Q02241
  • 基因名：
    KIF23
  • 别名：
    CHO 1 antibody; CHO1 antibody; KIF 23 antibody; KIF23 antibody; KIF23_HUMAN antibody; Kinesin family member 23 antibody; Kinesin like 5 antibody; Kinesin like protein 5 antibody; Kinesin like protein KIF 23 antibody; Kinesin like protein KIF23 antibody; Kinesin-like protein 5 antibody; Kinesin-like protein KIF23 antibody; KNS L5 antibody; KNSL 5 antibody; KNSL5 antibody; Mitotic kinesin like 1 antibody; Mitotic kinesin like protein 1 antibody; Mitotic kinesin-like protein 1 antibody; MKLP 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Kinesin-like protein KIF23 protein (865-960AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，KIF23 Antibody (CSB-PA23569A0Rb)，的标记方式是Non-conjugated。对于KIF23 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA23569B0Rb KIF23 Antibody, HRP conjugated ELISA
    FITC CSB-PA23569C0Rb KIF23 Antibody, FITC conjugated
    Biotin CSB-PA23569D0Rb KIF23 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
  • 基因功能参考文献：
    1. Data show that p120-catenin interacts with kinesin family member 23 (MKLP1) to regulate focused rhoA GTP-binding protein (RhoA) activity during cytokinesis. PMID: 28004812
    2. KIF23 was found highly expressed in NSCLC PMID: 29277180
    3. Results show the mitotic regulators KIF23 and MIS18BP1 to be co-modified by SUMO and ubiquitin on inhibition of the proteasome and subsequently identified as novel RNF4 targets. PMID: 28951443
    4. s found that KIF23 was regulated by TCF-4 at transcriptionally level. Therefore, this evidence indicates KIF23 over-expression is associated with glioma malignancy and conferred a worse survival time in glioma. PMID: 27013586
    5. Data show high level of KIF23 expression in the majority of primary and metastatic lung cancer tissues or cell lines and associates with poor survival. PMID: 26775597
    6. In this study we show for the first time that KIF23 V1 and V2 have different localizations in hepatocellular carcinoma cells PMID: 26674738
    7. TRAF6 mediates ubiquitination of the midbody ring localized protein KIF23/MKLP1. PMID: 24128730
    8. Transcriptional regulation of KIF23 is mediated by TP53. PMID: 23650552
    9. KIF23 mutation is associated with congenital dyserythropoietic anemia type III. PMID: 23570799
    10. It was shown that, during cytokinesis, Arf6 is first accumulated around the cleavage furrow and, prior to abscission, recruited onto the Flemming body via interaction with MKLP1. PMID: 22522702
    11. The results of this study indicated that downregulation of KIF23 decreases proliferation of glioma cells and that KIF23 may be a novel therapeutic target in malignant glioma. PMID: 21904957
    12. Study shows that 14-3-3 protein binds centralspindlin when the kinesin-6 component MKLP1 is phosphorylated at S710; 14-3-3 prevents centralspindlin from clustering in vitro, and MKLP1 mutant that is unable to bind 14-3-3 forms aberrant clusters in vivo. PMID: 20451386
    13. CHO1 and its truncated isoform MKLP1 are coexpressed in a single cell. The sequence encoded by exon 18 possesses a capability to interact with F-actin, suggesting that CHO1 can associate with both microtubule and actin cytoskeletons. PMID: 11877456
    14. Plk1 and CHO1/MKLP-1 interact to have a role in cytokinesis PMID: 15199097
    15. Recruitment of MKLP1 to the midzone/midbody by INCENP is a crucial step for the midbody formation and completion of cytokinesis in mammalian cells. PMID: 15796717
    16. Spatial restriction of Aurora B to the central spindle by MKlp2 regulates MKlp1 during cytokinesis in human cells. PMID: 16461284
    17. Cep55 directly binds to MKLP1 in vitro and associates with the MKLP1-MgcRacGAP centralspindlin complex in vivo PMID: 16790497
    18. Ectopic expression of a mutant Mklp-1 without the nuclear localization signal(s)(NLSs) leads to cell cycle arrest at cytokinesis, indicating that the NLSs are necessary for Mklp-1 to execute its normal function during cell division. PMID: 17198681
    19. study reports that mitotic complex genes Ect2, RacGAP, and MKLP1 are coordinately induced in S phase in proliferating T lymphocytes as well as in epithelial cells, depending upon activity of the CUX1 and E2F1 transcription factors PMID: 19015243
    20. MKLP1 interacts with CYK4, a GTPase activating protein for the Rho GTPase, to form the centralspindlin complex. Centralspindlin is a key regulator of Rho function and microtubule organisation during cytokinesis, and is essential for cell division. PMID: 11782313

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Midbody, Midbody ring. Note=Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family
  • 数据库链接：

    HGNC: 6392

    OMIM: 605064

    KEGG: hsa:9493

    STRING: 9606.ENSP00000260363

    UniGene: Hs.270845