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KARS Antibody

  • 货号：
    CSB-PA056745
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA056745(KARS Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA056745(KARS Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q15046
  • 基因名：
    KARS
  • 别名：
    CMTRIB antibody; DFNB89 antibody; EC 6.1.1.6 antibody; KARS 1 antibody; KARS 2 antibody; KARS antibody; KARS1 antibody; KARS2 antibody; KIAA0070 antibody; KRS antibody; Lysine tRNA ligase antibody; Lysine--tRNA ligase antibody; LysRS antibody; Lysyl tRNA synthetase antibody; Lysyl-tRNA synthetase antibody; SYK_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Fusion protein of Human KARS
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.; (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
  • 基因功能参考文献：
    1. The studies showed that mutations in KARS lead to a newly defined subtype of leukoencephalopathy associated with sensorineural hearing impairment. The combined effect of reduced aminoacylation and release of LysRS from the multiple-synthetase complex (MSC) likely underlies the pathogenesis of the KARS mutations identified in this study. PMID: 28887846
    2. Using the TruSight One sequencing panel we identified two novel mutations in KARS. Both mutations, never reported previously, occur in a highly conserved region of the catalytic domain and displayed a dramatic effect on KARS stability PMID: 27891585
    3. Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from colorectal cancer cells. PMID: 28611052
    4. Results indicate that KRS can promote cell-cell and cellextracellular matrix adhesion for migration. PMID: 26891990
    5. finding show that enzymatically active Shiga toxins trigger the dissociation of lysyl-tRNA synthetase (KRS) from the multi-aminoacyl-tRNA synthetase complex in human macrophage-like differentiated THP-1 cells and its subsequent secretion. PMID: 26643967
    6. KRS at the plasma membrane plays new roles in metastatic migration as a signaling inducer, and causes intracellular signaling for cancer dissemination PMID: 26091349
    7. tRK1 forms a complex with human enolases and interacts with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2) PMID: 25918939
    8. structural characteristics of the KRS-LR interaction on the cell surface PMID: 24983501
    9. Lysyl-tRNA synthetase plays essential role in HIV replication, transcriptional regulation, cytokine-like signaling. [review] PMID: 23972532
    10. The role of preKARS2 in the tRNA mitochondrial import. PMID: 23799079
    11. The KARS variant is identified in two families affected by DFNB89-associated autosomal-recessive nonsyndromic hearing impairment. PMID: 23768514
    12. C-terminal domain of HIV-1 capsid protein as surrogate for human lysyl tRNA synthetase PMID: 23208549
    13. A single conformational change triggered by phosphorylation leads to multiple effects driving an exclusive switch of LysRS function from translation to transcription. PMID: 23159739
    14. Dual role for motif 1 residues of human lysyl-tRNA synthetase in dimerization and packaging into HIV-1. PMID: 23095741
    15. The work thus unveiled a unique function of KRS in the control of cell migration and its pathological implication in metastasis. PMID: 22751010
    16. Data are consistent with hypothesis that maturation of cytoplasmic KARS precursor is needed to reveal the potent tRNA binding properties of mitochondrial KARS. PMID: 22235746
    17. LysRS associates with the Pol domain of GagPol. PMID: 21763493
    18. The results suggest that this unique geometry, which reconfigures the LysRS tetramer from alpha(2):alpha(2) to alpha(2)beta(1):beta(1)alpha(2), is designed to control both retention and mobilization of LysRS from the multi-tRNA synthetase complex PMID: 21536907
    19. The interaction between helix 7 of LysRS and helix 4 of the capsid C-terminal domain of HIV-1 Gag (HIV-CA-CTD) was studied using circular dichromism spectroscopy and molecular dynamics simulation. PMID: 21093454
    20. These results underscore the contribution of KARS to the emission of (one of) the principal signal(s) of immunogenic cell death, CRT exposure. PMID: 20699648
    21. Loss-of-function lysyl-tRNA synthetase mutations associated with peripheral neuropathy and Charcot-Marie-Tooth disease. PMID: 20920668
    22. HIV-1 Gag interacts with human lysyl-tRNA synthetase during viral assembly PMID: 12756246
    23. packaged into human immunodeficiency virus type 1 (HIV-1) via its interaction with Gag; this enzyme facilitates the selective packaging of tRNA(3)(Lys), the primer for initiating reverse transcription, into HIV-1 PMID: 15220430
    24. analysis of the interaction between HIV-1 Gag and human lysyl-tRNA synthetase PMID: 16702215
    25. HIV-1 Vpr fulfills an essential role in the process of packaging of mitochondrial Lysyl-tRNA synthase. PMID: 17560997
    26. This work reports further characterization of the interaction between HIV-1 capsid domain of Gag and human LysRS using truncation constructs and point mutations in the putative interaction helices. PMID: 17724017
    27. present a 2.3-A crystal structure of a tetrameric form of human LysRS PMID: 18272479
    28. mitoKARS is the first described member of a group of mitochondrial proteins whose interaction with mutant SOD1 contributes to mitochondrial dysfunction in ALS PMID: 18715867

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  • 相关疾病：
    Charcot-Marie-Tooth disease, recessive, intermediate type, B (CMTRIB); Deafness, autosomal recessive, 89 (DFNB89)
  • 亚细胞定位：
    [Isoform Cytoplasmic]: Cytoplasm, cytosol. Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted.; [Isoform Mitochondrial]: Mitochondrion.
  • 蛋白家族：
    Class-II aminoacyl-tRNA synthetase family
  • 数据库链接：

    HGNC: 6215

    OMIM: 601421

    KEGG: hsa:3735

    STRING: 9606.ENSP00000325448

    UniGene: Hs.3100