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IL4I1 Antibody

  • 货号：
    CSB-PA850426LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse stomach tissue
      All lanes: IL4I1 antibody at 3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 63, 66 kDa
      Observed band size: 63 kDa
    • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA850426LA01HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA850426LA01HU at dilution of 1:100
  • 其他：

产品详情

  • 产品描述：

    The recombinant human L-amino-acid oxidase protein (139-339aa) was injected into a rabbit to trigger an immune response leading to the generation of antibodies against IL4I1. The IL4I1 polyclonal antibody was isolated and purified from the rabbit's serum through protein G affinity chromatography. Its purity exceeds 95%. This rabbit anti-human IL4I1 polyclonal antibody was tested in the ELISA, WB, and IHC. The non-conjugated IgG was purified by protein G affinity chromatography and reached up to 95% in purity. It shows reactivity with the human and mouse IL4I1 proteins and may be used to detect the endogenous levels of IL4I1 protein.

    The target protein IL4I1 is primarily responsible for the degradation of L-tryptophan, which has important implications for the immune response, tissue repair and regeneration, and tumor growth and metastasis.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) IL4I1 Polyclonal antibody
  • Uniprot No.：
    Q96RQ9
  • 基因名：
    IL4I1
  • 别名：
    IL4I1 antibody; FIG1 antibody; UNQ636/PRO1265 antibody; L-amino-acid oxidase antibody; LAAO antibody; LAO antibody; EC 1.4.3.2 antibody; Interleukin-4-induced protein 1 antibody; IL4-induced protein 1 antibody; Protein Fig-1 antibody; hFIG1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human L-amino-acid oxidase protein (139-339AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，IL4I1 Antibody (CSB-PA850426LA01HU)，的标记方式是Non-conjugated。对于IL4I1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA850426LB01HU IL4I1 Antibody, HRP conjugated ELISA
    FITC CSB-PA850426LC01HU IL4I1 Antibody, FITC conjugated
    Biotin CSB-PA850426LD01HU IL4I1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:2000-1:10000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cells, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2.
  • 基因功能参考文献：
    1. the presence of IL4I1 during T-cell activation decreases early signaling events downstream of t-cell receptor stimulation, resulting in global T-cell inhibition which is more pronounced when there is CD28 costimulation. PMID: 28891065
    2. Data showed proteome changes in alveolar epithelial cells type II exposed to an infection with A. fumigatus with IL4I1 showing the most prominent increase in abundance both, in terms of relative abundance as well as activity. Also, in the infected lungs, there was levels of IL4I1 metabolic products. PMID: 28951444
    3. reviewed SNPs of the IL4I1 isoform 1, which is expressed in lymphoid tissue; the N92D SNP leads to a hyperactive enzyme, while the R102G mutation is hypomorphic; show that IL4I1 activity is not only directed against phenylalanine, as initially described, but also at a lower level against arginine PMID: 26673964
    4. The immunosuppressive enzyme IL4I1 expressed differentially in human induced Aiolos+, but not natural Helios+, FOXP3+ Treg cells. PMID: 25446972
    5. IL4I1 upregulation in human Th17 cells limits their TCR-mediated expansion not only by blocking the molecular pathway involved in the activation of the IL-2 promoter, but also by maintaining high levels of Tob1, which impairs entry into the cell cycle. PMID: 24307243
    6. IL4I1 plays a distinct role compared to other antibacterial enzymes produced by mononuclear phagocytes. PMID: 23355881
    7. participates in the downregulation of Th1 inflammation PMID: 20683900
    8. genomic structure, cDNA sequence, chromosome location and RNA expression in immune tissues PMID: 12031486
    9. is activated in primary mediastinal large B-cell lymphoma, which might be due to a constitutive activation of a cytokine signaling pathway PMID: 12446450
    10. hIL4I1 inhibited the proliferation of CD3-stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells. In contrast, memory T cells were more strongly affected by hIL4I1 and its catabolite H(2)O(2) than naive T cells PMID: 17356132
    11. strong IL4I1 expression is associated with B-cell lymphomas. PMID: 19436310

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  • 亚细胞定位：
    Secreted. Lysosome. Cytoplasmic vesicle, secretory vesicle, acrosome.
  • 蛋白家族：
    Flavin monoamine oxidase family, FIG1 subfamily
  • 组织特异性：
    Primarily found in immune tissues, with the highest expression in lymph nodes and spleen. Present in germinal center macrophages and inflammatory myeloid cells and antigen-presenting cells (at protein level). Also present in spermatozoa (at protein level)
  • 数据库链接：

    HGNC: 19094

    OMIM: 609742

    KEGG: hsa:259307

    STRING: 9606.ENSP00000342557

    UniGene: Hs.574492