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HPN Antibody

  • 货号：
    CSB-PA010704LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue, Mouse liver tissue
      All lanes: HPN antibody at 4.5µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 46 kDa
      Observed band size: 46 kDa
    • IHC image of CSB-PA010704LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of MCF-7 cells with CSB-PA010704LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The HPN polyclonal antibody is a rabbit antibody that specifically recognizes HPN proteins from human, mouse, and rat samples. Its immunogen is the recombinant human serine protease hepsin protein (294-413aa). It has been validated for use in ELISA, WB, IHC, and IF assays. The antibody has been purified using protein G and has a purity level of over 95%.

    Hepsin is a type II transmembrane serine protease that plays a key role in several physiological processes, including the regulation of blood coagulation, cell growth and differentiation, and tumor progression. It is involved in the progression of several types of cancer, including prostate, breast, and ovarian cancer.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HPN Polyclonal antibody
  • Uniprot No.：
    P05981
  • 基因名：
    HPN
  • 别名：
    HPN; TMPRSS1; Serine protease hepsin; Transmembrane protease serine 1
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human Serine protease hepsin protein (294-413AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，HPN Antibody (CSB-PA010704LA01HU)，的标记方式是Non-conjugated。对于HPN Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA010704LB01HU HPN Antibody, HRP conjugated ELISA
    FITC CSB-PA010704LC01HU HPN Antibody, FITC conjugated
    Biotin CSB-PA010704LD01HU HPN Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine protease that cleaves extracellular substrates, and contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL. Plays a role in cell growth and maintenance of cell morphology. Plays a role in the proteolytic processing of ACE2. Mediates the proteolytic cleavage of urinary UMOD that is required for UMOD polymerization.
  • 基因功能参考文献：
    1. Study showed was that Hepsin was downregulated in 78% of gastric cancer. Its high expression seems to predict poor prognosis. These results predicted that hepsin protein expression was one of the significant and independent prognostic factors for overall survival of Gastric Cancer. PMID: 27841306
    2. Genetic variation in/near the gene encoding for hepsin protein may influence risk of bipolar disorder and (at least for the rs62122114-A allele) may have functional impact (i.e. differential expression) as evidenced by serum HPN protein expression. PMID: 28886448
    3. significant negative association was found between hepsin expression in endometrial carcinoma cases regarding the grade and the size of tumors as well as myometrial invasion PMID: 26990747
    4. These data demonstrate that the membrane-bound serine protease hepsin is the enzyme responsible for the physiological cleavage of uromodulin. PMID: 26673890
    5. The Hepsin pathway acts in concert with Wnt pathway to promote prostate cancer progression. PMID: 26139199
    6. Low expression levels of hepsin and TMPRSS3 are associated with poor breast cancer survival PMID: 26014348
    7. Hepsin suppressed CDK11p58 internal ribosome entry site activity in prostate cancer cells by modulating UNR expression and eIF-2alpha phosphorylation. PMID: 25576733
    8. The results of this study suggest that, in Korean men, some polymorphisms in the HPN gene might be associated with the risk of developing prostate cancer. PMID: 22665141
    9. hepsin expression is frequently up-regulated in breast cancer tissues, which is associated with tumor growth and progression PMID: 21383634
    10. These findings suggest that the MSP/RON signaling pathway may be regulated by hepsin in tissue homeostasis and in disease pathologies, such as in cancer and immune disorders. PMID: 21875933
    11. Elevated levels of hepsin interfere with cell adhesion and viability in the background of prostate cancer as well as other tissue types, the details of which depend on the microenvironment provided. PMID: 21750652
    12. Hepsin activity was inhibited by anthralin and increased by resveratrol. PMID: 20673210
    13. Germline genetic variation of HPN does not seem to contribute to risk of prostate cancer or prognosis. PMID: 20166135
    14. Hepsin activates prostasin and cleaves the extracellular domain of the epidermal growth factor receptor. PMID: 19911255
    15. Hepsin and maspin are inversely expressed in laser capture microdissectioned prostate cancer PMID: 12629351
    16. Hepsin is functionally linked to hepatocyte growth factor/MET pathway, which may contribute to prostate cancer progression. PMID: 15792801
    17. A major 11-locus haplotype is significantly associated with prostate cancer, which provides further support that HPN is a potentially important candidate gene involved in prostate cancer susceptibility. PMID: 16783571
    18. the ability of hepsin to efficiently activate pro-uPA suggests that it may initiate plasmin-mediated proteolytic pathways at the tumor/stroma interface that lead to basement membrane disruption and tumor progression PMID: 16908524
    19. Our findings suggest that the balance between hepsin and its inhibitor, HAI-2, may have prognostic value in RCC. PMID: 17309599
    20. We analyzed all the common variations using tag SNP in the HPN and LTBP4 genes for association with IA in 390 patients and 642 controls in the Dutch population. PMID: 18487557
    21. Hepsin showed potential inhibitory effects mediated by the induction of 14-3-3sigma expression which leads to both cell cycle arrest at the G2/M phase PMID: 18698500
    22. Ln-332 may be one mechanism by which hepsin promotes prostate tumor progression and metastasis, possibly by up-regulating prostate cancer cell motility. PMID: 18784072

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Detected in liver and kidney.
  • 数据库链接：

    HGNC: 5155

    OMIM: 142440

    KEGG: hsa:3249

    STRING: 9606.ENSP00000262626

    UniGene: Hs.182385