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HMGB3 Antibody

  • 货号：
    CSB-PA010561LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate
      All lanes: HMGB3 antibody at 7µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 23 kDa
      Observed band size: 23 kDa
    • IHC image of CSB-PA010561LA01HU diluted at 1:300 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA010561LA01HU diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HMGB3 Polyclonal antibody
  • Uniprot No.：
    O15347
  • 基因名：
    HMGB3
  • 别名：
    chromosomal protein; Nonhistone; HMG4 antibody; High mobility group (nonhistone chromosomal) protein 4 antibody; High mobility group box 3 antibody; High mobility group protein 2a antibody; High mobility group protein 4 antibody; High mobility group protein B3 antibody; High mobility group protein HMG4 antibody; HMG 4 antibody; HMG-2a antibody; HMG-4 antibody; HMG2A antibody; HMGB 3 antibody; Hmgb3 antibody; HMGB3_HUMAN antibody; MGC90319 antibody; Non histone chromosomal protein antibody; Nonhistone chromosomal protein HMG4 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human High mobility group protein B3 protein (1-103AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，HMGB3 Antibody (CSB-PA010561LA01HU)，的标记方式是Non-conjugated。对于HMGB3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA010561LB01HU HMGB3 Antibody, HRP conjugated ELISA
    FITC CSB-PA010561LC01HU HMGB3 Antibody, FITC conjugated
    Biotin CSB-PA010561LD01HU HMGB3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling.
  • 基因功能参考文献：
    1. HMGB3 is involved in malignant transformation of hepatocytes and could be a useful biomarker for diagnosis and a potential target for therapy of liver cancer. PMID: 30166860
    2. The study strongly suggests that HMGB3 promotes GBM oncogenesis through the MAPK signalling pathway while miR-200b-3p and miR-200c-3p inhibit its expression. PMID: 30232806
    3. Luciferase reporter assays showed that HMGB3 was directly regulated by miR-205-5p in prostate cancer cells. PMID: 29196733
    4. HMGB3 regulates the genes expression of WNT/beta-catenin pathway PMID: 28678825
    5. Therefore, we infer that HMGB3 is a functional target of miR-27b in modulation of tamoxifen resistance and EMT. PMID: 27363334
    6. these findings demonstrate that HMBG3 may be a potential molecular marker for predicting the prognosis of esophageal squamous cell carcinoma patients PMID: 25755721
    7. our data suggests HMGB3 may serve as an important oncoprotein and indicate that overexpression of HMGB3 in urinary bladder cancer could be used as a potential prognostic marker. PMID: 25647262
    8. High expression of HMGB3 is associated with lymph node metastases in gastric cancer. PMID: 25095979
    9. In this family, microphthalmia, microcephaly, intellectual disability, and short stature are associated with a mutation on the X chromosome in the HMGB3 gene. PMID: 24993872
    10. regulation of HMGB3 by miR-205 reduced both proliferation and invasion of breast cancer cells. PMID: 24098490
    11. Association between co-expression networks involving HMGB3 and differentially-expressed genes in gastric cancer. PMID: 24338397
    12. HMGB3 has a role in gastric cancer disease onset and progression by regulating the cell cycle PMID: 23326140
    13. HMGB3 plays an important role in non-small cell lung cancer progression. PMID: 23609034
    14. Data indicate that myomiR-206 modulates Hmgb3 expression during myogenesis. PMID: 22912879

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  • 相关疾病：
    Microphthalmia, syndromic, 13 (MCOPS13)
  • 亚细胞定位：
    Nucleus. Chromosome. Cytoplasm.
  • 蛋白家族：
    HMGB family
  • 组织特异性：
    Expressed predominantly in placenta.
  • 数据库链接：

    HGNC: 5004

    OMIM: 300193

    KEGG: hsa:3149

    STRING: 9606.ENSP00000359393

    UniGene: Hs.19114