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  4. HMGB2 Antibody

HMGB2 Antibody

  • 货号：
    CSB-PA002938
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of COLO205 cells using HMG-2 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    P26583
  • 基因名：
    HMGB2
  • 别名：
    C80539 antibody; High mobility group (nonhistone chromosomal) protein 2 antibody; High mobility group box 2 antibody; High mobility group protein 2 antibody; High mobility group protein B2 antibody; HMG 2 antibody; HMG B2 antibody; HMG-2 antibody; HMG2 antibody; HMGB2 antibody; HMGB2_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human HMG-2.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:10000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity. Involved in V(D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors. In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE. Has antimicrobial activity in gastrointestinal epithelial tissues. Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity. Involved in modulation of neurogenesis probably by regulation of neural stem proliferation. Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway.
  • 基因功能参考文献：
    1. HMBG2 overexpression promotes ischemia/reperfusion-induced cell apoptosis through activating the JNK1/2-NF-kappaBp65 signaling in AC16 cardiomyocytes. PMID: 30119172
    2. s demonstrate that HMGB2 transcription is repressed by p21 during radiation-induced senescence through the ATM-p53-p21 DNA damage signaling cascade. The loss of p21 abolished the downregulation of HMGB2 caused by ionizing radiation, and the conditional induction of p21 was sufficient to repress the transcription of HMGB2. PMID: 29487276
    3. identified the oncogene HMGB2 as a downstream target of miR-130a by using luciferase and western blot assays. Knockdown of HMGB2 mimicked the effect of miR-130a in glioma cells. PMID: 28851665
    4. Serum HMGB2 levels were associated with in-stent restenosis in patients. PMID: 28183701
    5. results establish HMGB2 as a novel master regulator that orchestrates senescence-associated secretory phenotype. PMID: 27799366
    6. Data indicate the function of high mobility group box 2 (HMGB2) in glycolytic control in pancreatic cancer. PMID: 28069585
    7. data demonstrate a reciprocal relationship between Hmgb2 and Ctcf in controlling aspects of chromatin structure and gene expression. PMID: 27226577
    8. Lrp1-antisense directly binds to high-mobility group box 2 (Hmgb2) and inhibits the activity of Hmgb2 to enhance Srebp1a-dependent transcription of Lrp1. PMID: 25937287
    9. Data show that odulation of RNA helicase DDX18 directly affects growth of tamoxifen-resistant cells, suggesting that it may be a critical downstream effector of the estrogen receptors (ERs) and high mobility group box 2 (HMGB2) complex. PMID: 25284587
    10. siRNA-mediated silencing of HMGB2 increased the sensitivity of the head and neck squamous cell carcinoma cell lines to cisplatin and 5-fluorouracil. PMID: 25327479
    11. The present data suggest that HMGB2 expression is a significant prognostic factor for glioblastoma and might play an important role in cell invasion. PMID: 23828241
    12. HMGB2 is necessary to protect colorectal cancer cells from DNA damage and efficient DNA repair and p53-mediated downregulation is a critical mechanism of modulating HMGB2 expression. PMID: 23255232
    13. Transient local HMGB2-DNA contacts dominate the DNA-bending mechanism used by HMGB proteins to increase DNA flexibility. PMID: 23143110
    14. The age-related loss of HMGB2 in articular cartilage may represent a mechanism responsible for the decline in adult cartilage stem cell populations. PMID: 21890638
    15. The results can partly be explained by altered cell proliferations by HMGB2 associated with the antiapoptotic pathway. PMID: 20851854
    16. Our findings collectively suggest that HMGB2 could stabilize p53 by interfering with E6/E6AP-mediated p53 degradation in HPV-positive HeLa cells. PMID: 20036050
    17. HMGB2 potentiates GATA-1-dependent transcription of GFI1B by Oct-1 and thereby controls erythroid differentiation. PMID: 19965638
    18. HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T-lymphocyte protease granzyme A. PMID: 11909973
    19. binds specifically to the first AT-rich region flanking the hypersensitive site 2 core sequence of the human beta-globin gene locus control region PMID: 12555809
    20. biophysical analysis of HMG domain from human HMGB2 binding to DNA PMID: 15833996
    21. HMG2 was determined in humans. PMID: 17406091
    22. single and double box proteins increase DNA flexibility and stability, albeit both effects are achieved at much lower protein concentrations for the double box. PMID: 17964600
    23. In human and murine cartilage, there is an aging-related loss of HMGB2 expression, ultimately leading to its complete absence. PMID: 19139395
    24. An isolated HMG box A domain from human HMGB2 does not enhance DNA flexibility. Substitution of a small number of cationic residues from the N-terminal leader of a functional yeast box B protein, Nhp6Ap, confers the ability to enhance DNA flexibility. PMID: 19236006

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  • 亚细胞定位：
    Nucleus. Chromosome. Cytoplasm. Secreted.
  • 蛋白家族：
    HMGB family
  • 组织特异性：
    Expressed in gastric and intestinal tissues (at protein level).
  • 数据库链接：

    HGNC: 5000

    OMIM: 163906

    KEGG: hsa:3148

    STRING: 9606.ENSP00000296503

    UniGene: Hs.434953