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HLTF Antibody

  • 货号：
    CSB-PA010520LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate
      All lanes: HLTF antibody at 3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 114, 100 kDa
      Observed band size: 114 kDa
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA010520LA01HU at dilution of 1:100
    • Immunoprecipitating HLTF in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA010520LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
      Lane 2: CSB-PA010520LA01HU (6μg) + Hela whole cell lysate (1mg)
      Lane 3: Hela whole cell lysate (20μg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HLTF Polyclonal antibody
  • Uniprot No.：
    Q14527
  • 基因名：
    HLTF
  • 别名：
    DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody; Helicase like transcription factor antibody; Helicase-like transcription factor antibody; HIP116 antibody; HIP116A antibody; HLTF 1 antibody; Hltf antibody; HLTF_HUMAN antibody; HLTF1 antibody; p113 antibody; RING finger protein 80 antibody; RNF80 antibody; SMARC A3 antibody; SMARCA 3 antibody; SMARCA3 antibody; SNF2-like 3 antibody; SNF2L3 antibody; Sucrose nonfermenting protein 2 like 3 antibody; Sucrose nonfermenting protein 2-like 3 antibody; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3 antibody; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3 antibody; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 antibody; ZBU 1 antibody; ZBU1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Helicase-like transcription factor protein (332-476AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，HLTF Antibody (CSB-PA010520LA01HU)，的标记方式是Non-conjugated。对于HLTF Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA010520LB01HU HLTF Antibody, HRP conjugated ELISA
    FITC CSB-PA010520LC01HU HLTF Antibody, FITC conjugated
    Biotin CSB-PA010520LD01HU HLTF Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters. These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
  • 基因功能参考文献：
    1. Data suggest that HIV-1 vpr mediates polyubiquitination of HLTF by directly loading it onto C-terminal WD40 domain of DCAF1 in complex the CRL4, an E3 ubiquitin ligase. (vpr = vpr gene product of Human immunodeficiency virus 1; HLTF = human helicase like transcription factor; DCAF1 = human Vpr (HIV-1) binding protein; CRL4 = human E3 ubiquitin ligase CRL4) PMID: 29079575
    2. A low WT HLTF expression with a high I21R HLTF expression is associated with a poor disease-free survival PMID: 29661164
    3. the present study does not provide any strong evidence that PAI-1 gene variants are implicated in the risk of DR or the development of DR during T2DM course. PMID: 28632032
    4. A large number of SNF2 family, DNA and ATP-dependent motor proteins are needed during transcription, DNA replication, and DNA repair to manipulate protein-DNA interactions and change DNA structure. SMARCAL1, ZRANB3, and HLTF are three related members of this family with specialized functions that maintain genome stability during DNA replication. [review] PMID: 28954549
    5. depletion of SMARCAL1, a SNF2-family DNA translocase that remodels stalled forks, restores replication fork stability and reduces the formation of replication stress-induced DNA breaks and chromosomal aberrations in BRCA1/2-deficient cells. In addition to SMARCAL1, other SNF2-family fork remodelers, including ZRANB3 and HLTF, cause nascent DNA degradation and genomic instability PMID: 29053959
    6. HIV-1 vpr reprograms CRL4(DCAF1) E3 to direct HLTF for proteasome-dependent degradation independent from previously reported Vpr interactions with base excision repair enzyme uracil DNA glycosylase (UNG2) and crossover junction endonuclease MUS81, which Vpr also directs for degradation via CRL4(DCAF1) E3. PMID: 27335459
    7. Fasudil reduced LPS-mediated TF and PAI-1 expression and activity in PBMCs. These effects may partially be relevant to the clinical benefits of fasudil in the treatment of CAPD patients. PMID: 27756191
    8. HLTF is degraded in lymphocytic cells and macrophages infected with Vpr-expressing HIV-1. Our results reveal a previously unidentified strategy for HIV-1 to antagonize DNA repair in host cells. PMID: 27114546
    9. HLTF expression is altered in various cancers via two mechanisms: gene silencing through promoter hypermethylation or alternative mRNA splicing, which leads to the expression of truncated proteins that lack DNA repair domains. [review] PMID: 26472339
    10. HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. PMID: 26350214
    11. Findings indicate a mechanism of helicase-like transcription factor HLTF-mediated fork reversal and suggest the requirement for distinct fork remodeling activities in the cell. PMID: 26051180
    12. These results suggest that the HIRAN domain functions as a sensor to the 3'-end of the primer strand at the stalled replication fork and that the domain facilitates fork regression. PMID: 25858588
    13. Study demonstrates a correlation between HLTF expression level and thyroid neoplastic progression where three truncated forms are detected in thyroid carcinoma. PMID: 25005870
    14. We were able to provide evidence that methylation of HLTF and especially HPP1 detected in serum is strongly correlated with cell death in CRC using LDH as surrogate marker PMID: 24708595
    15. Results demonstrate that loss of HLTF function promotes the malignant transformation of intestinal or colonic adenomas to carcinomas by inducing genomic instability. PMID: 22452792
    16. results delineate a previously unknown USP7-HLTF-PCNA molecular network controlling DNA damage response PMID: 21845734
    17. HLTF can displace a broad spectrum of proteins such as replication protein A (RPA), PCNA, and replication factor C (RFC), thereby providing the first example for a protein clearing activity at the stalled replication fork. PMID: 21795603
    18. HLTF and SHPRH suppress mutagenesis in a damage-specific manner, preventing mutations induced by UV rays and methyl methanesulfonate. PMID: 21396873
    19. results suggest that HLTF is involved in DNA repair and apoptosis in cancer cells, which might represent a target for gene therapies of human cancer PMID: 20535496
    20. this is the first report identifying the regulatory mechanism of HLTF by CHFR, suggesting that CHFR-mediated downregulation of HLTF may help protect against cancer. PMID: 20388495
    21. HLTF can promote error-free replication of damaged DNA and support a role for HLTF in preventing mutagenesis and carcinogenesis, providing thereby for its potential tumor suppressor role. PMID: 19948885
    22. These results suggest that HLTF promoter hypermethylation is frequently demonstrated in human gastric cancer, and inactivation of HLTF or the chromatin-remodeling complex may play a crucial role in gastric carcinogenesis. PMID: 12766908
    23. Acetylation of histones H3 and H4 in the 5' CpG island of the HLTF gene were inversely associated with DNA methylation status. PMID: 12901794
    24. Methylation helicase-like transcription factor is associated with advanced esophageal squamous cell carcinoma PMID: 17094449
    25. HLTF activation was recently linked to the initial steps of carcinogenesis in an experimental model of estrogen-induced kidney tumor. [REVIEW] PMID: 18034322
    26. HLTF physically interacts with the Rad6-Rad18 and Mms2-Ubc13 ubiquitin-conjugating enzyme complexes and promotes the Lys-63-linked polyubiquitination of proliferating cell nuclear antigen at its Lys-164 residue. PMID: 18316726
    27. Splice arrays and RT-PCR showed that although most splice variants in RUSH and ATP11B are conserved in human and rabbit, the RFBP isoform is specific to rabbit. PMID: 18584949
    28. HLTF and SHPRH are functional homologues of yeast Rad5 that cooperatively mediate PCNA polyubiquitination and maintain genomic stability. PMID: 18719106
    29. There is an association between the presence of helicase-like transcription factor and neoplastic progression of hypopharyngeal and laryngeal squamous cell carcinomas. PMID: 18825407
    30. Hypopharyngeal squamous cell carcinomas presenting high levels of HLTF have a worse prognosis. PMID: 19614770
    31. biochemical characterisation of cellular HLTF is described. PMID: 19723507
    32. HLTF/SMARCA3, a member of SWI/SNF family, is a common target for methylation and epigenetic gene silencing in colon cancer and a candidate colon cancer suppressor gene. PMID: 11904375
    33. SMARCA3 is the human gene for RUSH transcription factors (U66564, U66565; Mol Endocrinol, 1996, 10:1335-1349). A binding partner has been cloned and characterized (AF236061; JBC, 2001, 276:3641-3649). PMID: 11058586

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm.
  • 蛋白家族：
    SNF2/RAD54 helicase family, RAD16 subfamily
  • 组织特异性：
    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • 数据库链接：

    HGNC: 11099

    OMIM: 603257

    KEGG: hsa:6596

    STRING: 9606.ENSP00000308944

    UniGene: Hs.3068