HIST1H2AG (Ab-118) Antibody
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货号:CSB-PA010389OA118nme1HU
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规格:¥660
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促销:
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图片:
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Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
All lanes: HIST1H2AG antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa -
Immunoprecipitating HIST1H2AG in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010389OA118nme1HU in NIH/3T3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010389OA118nme1HU (5µg) + NIH/3T3 whole cell lysate (500µg)
Lane 3: NIH/3T3 whole cell lysate (20µg) -
Chromatin Immunoprecipitation Hela (106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2AG (CSB-PA010389OA118nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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其他:
产品详情
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产品名称:Rabbit anti-Homo sapiens (Human) HIST1H2AG Polyclonal antibody
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Uniprot No.:P0C0S8
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基因名:HIST1H2AG
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别名:H2AC11 antibody; H2AFP antibody; HIST1H2AG; antibody; H2AC13 antibody; H2AFC antibody; HIST1H2AI; antibody; H2AC15 antibody; H2AFD antibody; HIST1H2AK; antibody; H2AC16 antibody; H2AFI antibody; HIST1H2AL; antibody; H2AC17 antibody; H2AFN antibody; HIST1H2AMHistone H2A type 1 antibody; H2A.1 antibody; Histone H2A/ptl antibody
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宿主:Rabbit
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反应种属:Human, Mouse
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免疫原:Peptide sequence around site of Lys (118) derived from Human Histone H2A type 1
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免疫原种属:Homo sapiens (Human)
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标记方式:Non-conjugated
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克隆类型:Polyclonal
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抗体亚型:IgG
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纯化方式:Antigen Affinity Purified
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浓度:It differs from different batches. Please contact us to confirm it.
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保存缓冲液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
产品提供形式:Liquid
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应用范围:ELISA, WB, IP, ChIP
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推荐稀释比:
Application Recommended Dilution WB 1:100-1:1000 IP 1:200-1:2000 -
Protocols:
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储存条件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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靶点详情
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功能:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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亚细胞定位:Nucleus. Chromosome.
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蛋白家族:Histone H2A family
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数据库链接:
HGNC: 4737
OMIM: 602787
KEGG: hsa:8329
STRING: 9606.ENSP00000352627
UniGene: Hs.134999
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