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HDAC5 (Ab-498) Antibody

  • 货号：
    CSB-PA930838
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from 293 and HepG2 cells using HDAC5(Ab-498) Antibody.
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5(Ab-498) Antibody(left) or the same antibody preincubated with blocking peptide(right).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HDAC5 Polyclonal antibody
  • Uniprot No.：
    Q9UQL6
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around aa.496~500 (T-Q-S-S-P) derived from Human HDAC5/7.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.
  • 基因功能参考文献：
    1. These results demonstrate a previously unknown negative epigenetic regulation of hematopoietic stem cells (HSC) homing and engraftment by HDAC5, and allow for a new and simple translational strategy to enhance HSC transplantation. PMID: 30013077
    2. Collectively, these data indicate that vIRF3 alters global gene expression and induces a hypersprouting formation in an HDAC5-binding-dependent and lymphatic endothelial cell-specific manner, ultimately contributing to Kaposi's sarcoma-associated herpesvirus-associated pathogenesis. PMID: 29339432
    3. High HDAC5 expression is associated with invasion of lung cancer. PMID: 30066893
    4. HO-1 plays a key role in protecting tumor cells from apoptosis, in a process that involves Smad7 and HDAC4/5 in apoptosis of B-ALL cells PMID: 29886060
    5. These findings demonstrate a novel mechanism for deregulation of HDAC5 in non-small cell lung cancer (NSCLC)and suggest that miR5895p/HDAC5 pathway may represent a new prognostic biomarker and therapeutic target against NSCLC. PMID: 28440397
    6. HDAC5 was extensively expressed in human BC tissues, and high HDAC5 expression was associated with an inferior prognosis. PMID: 27177225
    7. HDAC5 is a negative predictor of disease-free and overall survival in pancreatic neuroendocrine tumor patients. PMID: 28235630
    8. Interference with both glucose and glutamine supply in HDAC5-inhibited cancer cells significantly increases apoptotic cell death. PMID: 28414307
    9. these results suggest that HDAC5 is critical in regulating LSD1 protein stability through post-translational modification, and the HDAC5-LSD1 axis has an important role in promoting breast cancer development and progression. PMID: 27212032
    10. The expression of HDAC5 was significantly increased in endothelial cells (ECs) from patients with systemic sclerosis (SSc) compared to healthy control endothelial cells. Silencing of HDAC5 in SSc ECs restored normal angiogenesis. HDAC5 knockdown followed by ATAC-seq assay in SSc ECs identified key HDAC5-regulated genes involved in angiogenesis and fibrosis, such as CYR61, PVRL2, and FSTL1. PMID: 27482699
    11. the migration and invasion of hepatocellular carcinoma cells were impaired by knockdown of histone deacetylase 5 or hypoxia-inducible factor-1alpha but rescued when eliminating homeodomain-interacting protein kinase-2 in hepatocellular carcinoma cells, which suggested the critical role of histone deacetylase 5-homeodomain-interacting protein kinase-2-hypoxia-inducible factor-1alpha pathway in hypoxia-induced metastasis. PMID: 28653891
    12. HDAC5 inhibits hepatic lipogenic genes expression by attenuating the transcriptional activity of liver X receptor. PMID: 27614433
    13. HDAC5 promotes cellular proliferation through the upregulation of cMet, and may provide a novel therapeutic target for the treatment of patients with Wilms' tumor. PMID: 26847592
    14. Formononetin-combined therapy may enhance the therapeutic efficacy of doxorubicin in glioma cells by preventing EMT through inhibition of HDAC5. PMID: 26261519
    15. These results suggest a strong regulatory function of HDAC5 in the pro-inflammatory response of macrophages. PMID: 26059794
    16. In erythroid cells, pull down experiments identified the presence of a novel complex formed by HDAC5, GATA1, EKLF and pERK which was instead undetectable in cells of the megakaryocytic lineage. PMID: 24594363
    17. Data reveal a novel role of HDAC5 in modulating the KLF2 transcriptional activation and eNOS expression. PMID: 25096223
    18. Studied phosphorylation sites within functional HDAC5 domains, including the deacetylation domain (DAC, Ser755), nuclear export signal (NES, Ser1108), and an acidic domain (AD, Ser611). PMID: 24920159
    19. mRNA and protein levels of HDAC5 were up-regulated in human hepatocellular carcinoma. PMID: 25129440
    20. HDAC5 promoted the Six1 expression. PMID: 24706304
    21. In C2C12 myoblasts, recombinant human HDAC5 phosphorylation by PKD regulated the expression of diverse metabolic genes and glucose metabolism. PMID: 24732133
    22. findings show N-Myc upregulated HDAC5 expression in neuroblastoma cells; HDAC5 repressed NEDD4 gene expression,increased Aurora A gene expression and consequently upregulated N-Myc protein expression;data identify HDAC5 as a novel co-factor in N-Myc oncogenesis PMID: 23812427
    23. we show that Stat3 binds to the promoter region of PTPN13 and promotes its activity through recruiting HDAC5. Thus, our results suggest a previously unknown Stat3-PTPN13 molecular network controlling squamous cell lung carcinoma development PMID: 24191246
    24. At the molecular level, we demonstrated that HDAC5 promoted mRNA expression of twist 1, which has been reported as an oncogene PMID: 24092570
    25. These findings suggest that HDAC5 is a key determinant of p53-mediated cell fate decisions in response to genotoxic stress. PMID: 24120667
    26. Data indicate there was a link between baseline viral load, age (40 years), IL-28B (rs12979860), HDAC2 (rs3778216), HDAC3 (rs976552) and HDAC5 (rs368328) with sustained virological response (SVR). PMID: 23615070
    27. HDAC5 is essential for the length maintenance of long telomeres and its depletion is required for sensitization of cancer cells with long telomeres to chemotherapy. PMID: 23729589
    28. Loss of HDAC5 impairs memory function but has little impact in a transgenic mouse model of amyloid pathology. PMID: 22914591
    29. Nuclear calcium signaling is a regulator of nuclear export of HDAC4 and HDAC5. PMID: 23364788
    30. Dephosphorylation at a conserved SP motif governs cAMP sensitivity and nuclear localization of class IIa histone deacetylases HDAC4, 5 and 9 PMID: 23297420
    31. Data suggest that HDAC5 regulates muscle glucose metabolism and insulin action and that HDAC inhibitors can be used to modulate these parameters in muscle cells. PMID: 22991226
    32. The current study identified the class II deacetylase HDAC5 as a novel promoting factor of CTG*CAG expansions. PMID: 22941650
    33. HDAC5 in the maintenance/assembly of pericentric heterochromatin structure and demonstrate that class IIa HDAC5 can represent a potential target for anticancer therapies. PMID: 22301920
    34. The results of this study suggested that suggest that HDAC5 provides a delayed braking mechanism on gene expression programs that support the development, but not expression, of cocaine reward behaviors. PMID: 22243750
    35. Significantly increased methylation of the HDAC5 gene was associated with astrocytomas. PMID: 21508384
    36. Ser279 is a critical phosphorylation within the NLS involved in the nuclear import of HDAC5 PMID: 21081666
    37. in addition to activation of protein kinase D isozymes by phosphorylating Ser744 and Ser748 at their activation sites, PKCdelta may also play a role in the regulation of HDAC5 by phosphorylation of Ser259 PMID: 21146494
    38. differentiation-dependent GLUT4 gene expression in 3T3-L1 adipocytes is dependent on the nuclear concentration of a class II histone deacetylase (HDAC) protein, HDAC5 PMID: 21047791
    39. Findings identify HDAC5 as a substrate of PKA and reveal a cAMP/PKA-dependent pathway that controls HDAC5 nucleocytoplasmic shuttling and represses gene transcription. PMID: 20716686
    40. phosphorylation-dependent derepression of HDAC5 mediates flow-induced KLF2 and eNOS expression as well as flow anti-inflammation, and suggest that HDAC5 could be a potential therapeutic target for the prevention of atherosclerosis. PMID: 20042720
    41. Class II histone deacetylases are directly recruited by BCL6 transcriptional repressor PMID: 11929873
    42. Histone deacetylase 5 is not a p53 target gene, but its overexpression inhibits tumor cell growth and induces apoptosis. PMID: 12019172
    43. MITR, HDAC4, and HDAC5 associate with heterochromatin protein 1 (HP1), an adaptor protein that recognizes methylated lysines within histone tails and mediates transcriptional repression by recruiting histone methyltransferase PMID: 12242305
    44. HDAC5 binds to Ca(2+)/calmodulin and inhibits MEF2a binding PMID: 12626519
    45. ICP0 of herpes simplex virus Type 1 is able to overcome the HDAC5 amino-terminal- and MITR-induced MEF2A repression in gene reporter assays PMID: 15194749
    46. The HDAC5, a class II HDAC involved in myogenesis, was not detected in the tissues. PMID: 15590418
    47. G betagamma binds HDAC5 and inhibits its transcriptional co-repression activity PMID: 16221676
    48. novel transcriptional pathway under the control of class II HDACs and suggest a role for these transcriptional repressors as signal-responsive regulators of antigen presentation PMID: 16236793
    49. NO-dependent PP2A activation plays a key role in nuclear translocation of class II HDACs HDAC4 and HDAC5 PMID: 17975112
    50. AMP-activated protein kinase (AMPK) regulates GLUT4 transcription through the histone deacetylase (HDAC)5 transcriptional repressor. PMID: 18184930

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.
  • 蛋白家族：
    Histone deacetylase family, HD type 2 subfamily
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 14068

    OMIM: 605315

    KEGG: hsa:10014

    STRING: 9606.ENSP00000225983

    UniGene: Hs.438782