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HCN1 Antibody

  • 货号：
    CSB-PA010216LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA010216LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA010216LA01HU diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA010216LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HCN1 Polyclonal antibody
  • Uniprot No.：
    O60741
  • 基因名：
    HCN1
  • 别名：
    BCNG-1 antibody; BCNG1 antibody; Brain cyclic nucleotide gated channel 1 antibody; Brain cyclic nucleotide-gated channel 1 antibody; HAC 2 antibody; HAC2 antibody; HCN1 antibody; HCN1_HUMAN antibody; Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody; Potassium channel; voltage-gated; brain; 1 antibody; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 protein (784-887AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，HCN1 Antibody (CSB-PA010216LA01HU)，的标记方式是Non-conjugated。对于HCN1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA010216LB01HU HCN1 Antibody, HRP conjugated ELISA
    FITC CSB-PA010216LC01HU HCN1 Antibody, FITC conjugated
    Biotin CSB-PA010216LD01HU HCN1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli.
  • 基因功能参考文献：
    1. Polymorphism of HCN1 is associated with breast cancer. PMID: 28178648
    2. Study presents cryo-electron microscopy structures of the human HCN1 channel in the absence and presence of cAMP at 3.5 A resolution. HCN channels contain a K(+) channel selectivity filter-forming sequence from which the amino acids create a unique structure that explains Na(+) and K(+) permeability. PMID: 28086084
    3. A new mode of regulating HCN1 trafficking: through the use of a di-arginine ER retention signal that monitors processing of the channel in the early secretory pathway. PMID: 25142030
    4. de novo HCN1 point mutations cause a recognizable early-onset epileptic encephalopathy in humans PMID: 24747641
    5. acute abrogation of HCN1-FLNa interaction in neurons, with the use of decoy peptides that mimic the FLNa-binding domain of HCN1, abolishes the punctate distribution of HCN1 channels in neuronal cell bodies PMID: 24403084
    6. Studies suggest that HCN1 channels may be therapeutic targets for treatment of depressive disorders. PMID: 23033536
    7. Wild-type presynaptic HCN1 channel function is persistently decreased following seizures. PMID: 23077068
    8. HCN1 channels make an important contribution to the maintenance of spontaneous burst activity in embryonic cortical neuron cultures. PMID: 22094222
    9. Hyperpolarization-activated currents are smaller and slower, input resistances are higher, and membrane time constants are longer in HCN1-deficient than in HCN1-expressing neurons of the ventral cochlear nucleus. PMID: 21562186
    10. Genetic analysis in 48 Sudden unexpected death in epilepsy cases identified six novel and three previously reported nonsynonymous (amino acid changing) variants in HCN1 , HCN2, HCN3 and HCN4. PMID: 21615589
    11. increasing cAMP levels in cells antagonized the up-regulation of HCN1 channels mediated by a TRIP8b construct binding the CNBD exclusively. PMID: 21504900
    12. Human HCN1 hyperpolarization activated current (Ih) amplitude is rapidly enhanced after establishment of the whole-cell configuration in HEK293 cells. PMID: 20806410
    13. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20145138
    14. Helical secondary structure of the external S3-S4 linker of this pacemaker channel is examined and affects its activation PMID: 12668666
    15. HCN1 and HCN2 expression were measured using in situ hybridization and immunocytochemistry in hippocampi; the expression of HCN isoforms is dynamically regulated in human as well as in experimental hippocampal epilepsy PMID: 12890777
    16. In HCN1, the amino acid substitution A881T was identified in one idiopathic generalized epilepsy patient. PMID: 17931874
    17. The polymorphism rs10941679 near HCN1/MRPS30 was also associated with percent dense area in breast cancer. PMID: 19232126
    18. Observational study of gene-disease association. (HuGE Navigator) PMID: 19232126
    19. Observational study of gene-disease association. (HuGE Navigator) PMID: 18454440
    20. Observational study of gene-disease association. (HuGE Navigator) PMID: 17931874
    21. Observational study of gene-disease association. (HuGE Navigator) PMID: 18081024

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  • 相关疾病：
    Epileptic encephalopathy, early infantile, 24 (EIEE24)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Potassium channel HCN family
  • 组织特异性：
    Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.
  • 数据库链接：

    HGNC: 4845

    OMIM: 602780

    KEGG: hsa:348980

    STRING: 9606.ENSP00000307342

    UniGene: Hs.353176