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  4. GRK5 Antibody

GRK5 Antibody

  • 货号：
    CSB-PA009926LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse kidney tissue
      All lanes: GRK5 antibody at 3.4μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 68 kDa
      Observed band size: 68 kDa
    • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA009926LA01HU at dilution of 1:100
    • Immunofluorescence staining of HepG2 cells with CSB-PA009926LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) GRK5 Polyclonal antibody
  • Uniprot No.：
    P34947
  • 基因名：
  • 别名：
    FLJ39780 antibody; FP2025 antibody; G protein coupled receptor kinase 5 antibody; G protein coupled receptor kinase GRK5 antibody; G protein-coupled receptor kinase 5 antibody; G protein-coupled receptor kinase GRK5 antibody; GPRK5 antibody; GRK-pan antibody; GRK5 antibody; GRK5_HUMAN antibody; Pan-GRK antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human G protein-coupled receptor kinase 5 protein (406-590AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，GRK5 Antibody (CSB-PA009926LA01HU)，的标记方式是Non-conjugated。对于GRK5 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA009926LB01HU GRK5 Antibody, HRP conjugated ELISA
    FITC CSB-PA009926LC01HU GRK5 Antibody, FITC conjugated
    Biotin CSB-PA009926LD01HU GRK5 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
  • 基因功能参考文献：
    1. There was no association of SNPs in ADRB1, GRK5 and BAG3 genes with Takotsubo cardiomyopathy. PMID: 29514624
    2. T2DM patients with genotypes CC and CT at GRK5 rs10886471 had a significant reduction in terms of fasting plasma glucose compared with those with genotype TT (p < .01). In addition, the carriers of genotypes CC and CT at GRK5 rs10886471 had higher differential values of postprandial serum insulin compared with genotype TT carriers (p < .05). PMID: 29663513
    3. Data indicate two moderately frequent variants in G protein-coupled receptor kinase 5 (GRK5) and seven very rare polymorphisms in patients with laterality defects (heterotaxy) that were associated with a congenital heart defect (CHD). PMID: 27618959
    4. demonstrate for the first time exosomal enrichment of G-protein-coupled receptor kinase (GRK) 5 and GRK6, both of which regulate Src and IGF-IR signaling and have been implicated in cancer. PMID: 27232975
    5. Data suggest that perturbation of interface formed between large lobe of kinase domain of GRK5 and the RHD (regulator of G protein signaling homology domain) leads to higher phosphorylation activity/activation of kinase activity. PMID: 28808053
    6. The role of GRK5 single nucleotide polymorphisms in postoperative atrial fibrillation susceptibility following coronary artery bypass grafting among Chinese population PMID: 26235218
    7. Candidate gene eQTL showed a trans-acting association between variants of G protein-coupled receptor kinase 5 gene, previously linked to altered BB response, and high expression of VOPP1. PMID: 26860460
    8. Further, we will also present potential therapeutic strategies (i.e. small molecule inhibition, gene therapy) that may have potential in combating the deleterious effects of GRK5 in heart failure. PMID: 26829117
    9. Suggest a common regulatory pattern for the beta(1)-AR/GRK5 which is independent of cellular type or pathology. PMID: 26248277
    10. We demonstrated that the SNPs and the related haplotypes of GRK5 could play a central role in predisposing Parkinson disease patients to cognitive impairment. PMID: 26614013
    11. ADORA2A and GRK5 gene variants may influence the etiology of malaria infection. PMID: 26066465
    12. A significant difference in the frequency of GRK5 polymorphism was found between Takotsubo cardiomyopathy patients and controls, supporting a genetic predisposition to this cardiac syndrome. PMID: 25010510
    13. Specific patterns of altered heart GRK5 gene expression were found in models of cardiac hypertrophy. PMID: 26515328
    14. This study sought to identify the distributed characteristics of these variant genotypes in Chinese population, and influence of GRK5 and ADRB1 polymorphisms on systolic heart failure morbidity. PMID: 25638254
    15. GRK5 is found in a partially closed state with its kinase domain C-tail forming novel interactions with nucleotide and the N-lobe. PMID: 26032409
    16. G protein-coupled receptor kinase 5 gene polymorphisms may have a role in postoperative atrial fibrillation after coronary artery bypass grafting in patients receiving beta-blockers PMID: 25049040
    17. These data suggest that increased GRK5 expression may contribute to the pathophysiology of schizophrenia. PMID: 25153362
    18. Results demonstrate crosstalk among WIP1, CXCR4 and GRK5, which may be important for the aggressive phenotype of a subclass of medulloblastomas in children. PMID: 24632620
    19. GRK5 dimerization is important for its plasma membrane localization and function. PMID: 24807909
    20. GRK5 intronic (CA)n polymorphisms associated with type 2 diabetes in Chinese Hainan Island. PMID: 24594703
    21. GRK5 regulates prostate cancer cell migration and invasion. GRK5 forms a complex with moesin, phosphorylates moesin principally on T66 residue, and regulates cellular distribution of moesin. PMID: 24755472
    22. genetic polymorophism is associated with plasma viscosity PMID: 24178511
    23. In the largest genotyped TC cohort in the literature, we have found no association of genetic variants in the ERalpha, beta1AR, beta2AR, or COMT genes, or with the previously implicated GRK5, with occurrence of the syndrome. PMID: 23794609
    24. we show, for the first time, that knocking down the expression of GRK5 decreased the proliferation rate of gliioblastoma stem cells in contrast to control. PMID: 23693024
    25. DNA-binding ability of GRK5 requires both the NLS and an N-terminal calmodulin (CaM)-binding site PMID: 23658733
    26. These data suggest cell type- and subcellular compartment-dependent differences in GRK/arrestin-mediated desensitization and signaling. PMID: 23139825
    27. A genome-wide association study identifies GRK5 and RASGRP1 as type 2 diabetes loci in Chinese Hans. PMID: 22961080
    28. GRK5 phosphorylates Ser-4 in nucleophosmin and regulates the sensitivity of cells to PLK1 inhibition. PMID: 22467873
    29. A reduced cortical concentration of GRK5 in schizophrenia (resembling that in aging) may result in altered G protein-dependent signaling, thus contributing to prefrontal deficits in schizophrenia. PMID: 21784156
    30. Increased GRK5 expression in the failing myocardium suggests a relevant role in human heart failure. PMID: 22196842
    31. GRK5 is localized in the centrosome and regulates microtubule nucleation and normal cell cycle progression. PMID: 22223642
    32. GRK5 is a transcriptional modifier of a subset of Galphaq-downregulated genes, acting in opposition to the pathological effects of Galphaq and normalizing levels of these transcripts. PMID: 21768220
    33. Hip has been identified as a novel substrate of GRK5 in vitro and in cells, and phosphorylation of Hip by GRK5 plays a role in modulating CXCR4 internalization PMID: 21728385
    34. GRK5 mediates cell growth suppression by TIG1A. Thus, TIG1 may participate in the downregulation of G-protein coupled signaling by upregulating GRK5 expression. PMID: 21575264
    35. GRK5 gene does not confer risk to sporadic Parkinson's disease in our sample from Southern Italy. PMID: 21184589
    36. The GRK5 Leu41 allele protects from adverse cardiovascular outcomes in treated hypertensives. PMID: 21127457
    37. Studies seem to indicate that mild, soluble, Beta-amyloid accumulation can lead to a reduced membrane (functional) and an elevated cytosolic GRK2/5. PMID: 20730384
    38. This study shows for the first time that GRK5 negatively regulates VEGF signaling in human coronary artery endothelial cells. PMID: 20443868
    39. GRK5 as a novel kinase of p53, as well as a negative regulator of p53-mediated signal transduction. PMID: 20124405
    40. it has been demonstrated that the GRK5 L41 variant causes a negative inotropic effect under conditions of acute catecholamine stimulationl8AAg PMID: 20023040
    41. GRK5 Gln41Leu polymorphism is not associated with sensitivity to beta(1)-adrenergic blockade in humans. PMID: 19842931
    42. This study uncovered previously unrecognized functionally important sites in the regulator of G-protein signaling homology domain of GRK5 kinase. PMID: 20038610
    43. Results identify GRK5/6 as novel kinases for the single transmembrane receptor LRP6 during Wnt signaling. PMID: 19801552
    44. Human substance P receptor undergoes agonist-dependent phosphorylation by G protein-coupled receptor kinase 5 in vitro. PMID: 12067742
    45. Data indicate that GRK5 does not regulate the sorting of human beta 2-adrenoceptors in the endocytic pathway. PMID: 14691047
    46. a group of hydrophobic amino acids within the membrane binding motif is critical to mediating the PM localization of GRK5 PMID: 14976207
    47. GRK5 has a DNA-binding nuclear localization sequence PMID: 15542828
    48. High expression was detected in septic neutrophils and control cells treated with cytokines plus LPS. PMID: 16849637
    49. Results show that GRK5 plays a distinctive role in the phosphorylation of the beta2AR. PMID: 18034461
    50. GRK5-Leu41 represents a gain-of-function polymorphism that evokes enhanced loss-of-function of beta2AR during persistent agonist exposure, and thus may contribute to beta-agonist variability in asthma treatment of African-Americans. PMID: 18622265

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily
  • 组织特异性：
    Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
  • 数据库链接：

    HGNC: 4544

    OMIM: 600870

    KEGG: hsa:2869

    STRING: 9606.ENSP00000376609

    UniGene: Hs.524625