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GRHL1 Antibody

  • 货号：
    CSB-PA868368LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
      All lanes: GRHL1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 71, 50, 58 kDa
      Observed band size: 71 kDa
    • IHC image of CSB-PA868368LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA868368LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) GRHL1 Polyclonal antibody
  • Uniprot No.：
    Q9NZI5
  • 基因名：
    GRHL1
  • 别名：
    Grainyhead-like protein 1 homolog antibody; grhl1 antibody; GRHL1_HUMAN antibody; LBP32 antibody; Mammalian grainyhead antibody; MGR antibody; NH32 antibody; TFCP2L2 antibody; Transcription factor CP2-like 2 antibody; Transcription factor LBP-32 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Grainyhead-like protein 1 homologprotein (1-95AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，GRHL1 Antibody (CSB-PA868368LA01HU)，的标记方式是Non-conjugated。对于GRHL1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA868368LB01HU GRHL1 Antibody, HRP conjugated ELISA
    FITC CSB-PA868368LC01HU GRHL1 Antibody, FITC conjugated
    Biotin CSB-PA868368LD01HU GRHL1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3'. Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development.; Functions as transcription activator.; May function as a repressor in tissues where both isoform 1 and isoform 2 are expressed.
  • 基因功能参考文献：
    1. Non-melanoma skin cancer growth is accompanied by coordinated reduced expression of epidermal differentiation genes: GRHL1 and GRHL3, which may be regulated by miR-21-3p and -5p, respectively. Some potentially damaging single nucleotide polymorphisms in GRHL genes occur with altered frequencies in NMSC patients, and they may in particular impair the expression of GRHL3 gene or functioning of encoded protein PMID: 29301499
    2. All of these processes involve epithelial-mesencyhmal transition (EMT), MET or a sequence of both, suggesting that the GRHL factors((GRHL1, GRHL2 and GRHL3), could potentially affect tumor initiation and progression via EMT PMID: 28714958
    3. it was observed that ER stimulated gene expression by interacting with MEIS1 and FOXP3, and ER inhibited gene expression by interacting with THRB and GRHL1. PMID: 27035558
    4. Grhl1 deficiency affects inner ear development in zebrafish. PMID: 25896282
    5. GRHL1, GRHL2, and GRHL3 have roles in cellular proliferation, differentiation, adhesion, and polarity and may promote cancer or be tumor suppressors [review] PMID: 26069269
    6. Downregulation of miR122 by grainyhead-like 2 restricts the hepatocytic differentiation potential of adult liver progenitor cells. PMID: 25406394
    7. A significant role for HDAC3 in the MYCN-mediated repression of GRHL1. PMID: 24419085
    8. Our findings reveal important pathophysiological differences between human pulmonary fibrosis and specific mouse models of fibrosis and support a crucial role of GRHL2 in epithelial activation in lung fibrosis and perhaps also in epithelial plasticity. PMID: 24375798
    9. LBP-1b and LBP-9 both stimulate LBP-32/MGR promoter activity. PMID: 18004979
    10. Includes a comparison to TFCP2L2, the GRHL1 protein. PMID: 12393799

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Grh/CP2 family, Grainyhead subfamily
  • 组织特异性：
    Isoform 1 is highly expressed in brain, pancreas, tonsil, placenta and kidney. Isoform 2 is highly expressed in brain and liver. Expressed at very low levels in non-steroidogenic cells.
  • 数据库链接：

    HGNC: 17923

    OMIM: 609786

    KEGG: hsa:29841

    STRING: 9606.ENSP00000324693

    UniGene: Hs.418493