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  4. GRB14 Antibody

GRB14 Antibody

  • 货号：
    CSB-PA900627
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA900627(GRB14 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA900627(GRB14 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
    • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human fetal intestines tissue, Primary antibody: CSB-PA900627(GRB14 Antibody) at dilution 1/350 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
  • 其他：

产品详情

  • Uniprot No.：
    Q14449
  • 基因名：
    GRB14
  • 别名：
    GRB14Growth factor receptor-bound protein 14 antibody; GRB14 adapter protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Fusion protein of Human GRB14
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    WB 1:200-1:1000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
  • 基因功能参考文献：
    1. We investigated the modification of air pollution and diabetes association by a genetic risk score covering 63 T2D genes. Five single variants near GRB14, UBE2E2, PTPRD, VPS26A and KCNQ1 showed nominally significant interactions with PM10 (P<0.05). Our results suggest that genetic risk for T2D may modify susceptibility to air pollution through alterations in insulin sensitivity. PMID: 27281273
    2. The N-terminus of the BPS domain plays an important role in the regulation of human Grb14 and insulin receptor complex formation through phosphorylation, in addition to other domains. PMID: 28130417
    3. Colorectal cancer patients with high GRB14 levels had a shorter survival and GRB14 was upregulated at an advanced clinical stage with enhanced tumor invasion and lymph node metastasis. PMID: 26965150
    4. Phosphorylation of Grb14 BPS domain by GSK-3 correlates with complex forming of Grb14 and insulin receptor. PMID: 24535599
    5. Genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits. Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14 PMID: 23670221
    6. Modulation of mouse rod photoreceptor responses by Grb14 protein. PMID: 24273167
    7. ANKRD55 rs459193 and GRB14 rs13389219 associate with insulin resistance. PMID: 23457408
    8. Grb14 is the first negative regulator of CEACAM3-initiated bacterial phagocytosis and might help to focus granulocyte responses to the subcellular sites of pathogen-host cell contact PMID: 22948154
    9. A new role for Grb14 in finely tuning receptor signaling and modulating thyroid cancer progression. PMID: 22158039
    10. serves as an adaptor protein to recruit 3-phosphoinositede-dependent kinase-1 to activated insulin receptor, thus promoting Akt phosphorylation and transduction of the insulin signal PMID: 15210700
    11. regulation of Grb14 expression levels in response to hormonal stimuli, and are consistent with its role as a repressor of insulin signaling where it is induced as a negative feedback mechanism. PMID: 15372466
    12. Grb14 may regulate signalling through the insulin receptor by controlling its tyrosine dephosphorylation in a site-specific manner. PMID: 16582879
    13. GRB14 is a weight-loss-responsive gene in skeletal muscle. Its observed transcriptional modulation may improve insulin signaling, with weight loss. PMID: 16849634
    14. Grb14 regulates insulin action at two levels, through insulin receptors binding and by interfering with downstream pathways. PMID: 19359342

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  • 亚细胞定位：
    Cytoplasm. Endosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    GRB7/10/14 family
  • 组织特异性：
    Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle.
  • 数据库链接：

    HGNC: 4565

    OMIM: 601524

    KEGG: hsa:2888

    STRING: 9606.ENSP00000263915

    UniGene: Hs.411881