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GPRC6A Antibody

  • 货号：
    CSB-PA722571LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, PC-3 whole cell lysate
      All lanes: GPRC6A antibody at 1:500
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 105, 85, 97 kDa
      Observed band size: 105 kDa
    • IHC image of CSB-PA722571LA01HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of PC-3 cells with CSB-PA722571LA01HU at 1:100, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cells with CSB-PA722571LA01HU at 1:100, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) GPRC6A Polyclonal antibody
  • Uniprot No.：
    Q5T6X5
  • 基因名：
  • 别名：
    bA86F4.3 antibody; G protein coupled receptor 33 antibody; G protein coupled receptor family C group 6 member A antibody; G-protein coupled receptor family C group 6 member A antibody; G-protein coupled receptor GPCR33 antibody; GPC6A_HUMAN antibody; GPCR 33 antibody; GPCR 6A antibody; GPCR antibody; GPCR33 antibody; GPCR6A antibody; GPR C6A antibody; GPRC 6A antibody; gprc6a antibody; hGPCR 33 antibody; hGPCR33 antibody; hGPRC6A antibody; OTTHUMP00000017075 antibody; Predicted with SOSUI analysis antibody; seven transmembrane helix receptor antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human G-protein coupled receptor family C group 6 member A protein
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，GPRC6A Antibody (CSB-PA722571LA01HU)，的标记方式是Non-conjugated。对于GPRC6A Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA722571LB01HU GPRC6A Antibody, HRP conjugated ELISA
    FITC CSB-PA722571LC01HU GPRC6A Antibody, FITC conjugated
    Biotin CSB-PA722571LD01HU GPRC6A Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium and osteocalcin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinate nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalent ions and its responsiveness to anabolic steroids.
  • 基因功能参考文献：
    1. If the regulatory functions of GPRC6A identified in mice translate to humans, and polymorphisms in GPRC6A are found to predict racial disparities in human diseases, GPRC6A may be a new gene target to predict, prevent, and treat MetS, PCa, and other disorders impacted by GPRC6A. PMID: 28180060
    2. Human GPRC6A is a functional osteocalcin and testosterone sensing receptor that promotes prostate cancer progression. PMID: 28659174
    3. he nonrare polymorphism rs2274911 in the GPRC6A gene was associated with insulin resistance features, independently of the metabolic phenotype and osteocalcin levels. PMID: 27696500
    4. Genetic Variations in the Human G Protein-coupled Receptor Class C, Group 6, Member A (GPRC6A) Control Cell Surface Expression and Function PMID: 27986810
    5. Osteocalcin and Sex Hormone Binding Globulin Compete on a Specific Binding Site of GPRC6A. PMID: 27673554
    6. results suggest that G protein-coupled receptor class C group 6 member A(GPRC6A) inactivation or sub-function contributes to reduced exposure to androgens, leading to cryptorchidism during fetal life and/or low sperm production in adulthood PMID: 26735260
    7. association of the THADA, FOXP4, GPRC6A/RFX6 and 8q24 genes with prostate cancer in Asian populations. PMID: 26537068
    8. The present experiments demonstrated a critical role for the GPRC6A-ERK1/2 and PI3K/Akt signaling pathway in arginine-mediated fibroblast survival. PMID: 24651445
    9. Duox1 activity is stimulated by testosterone through GPRC6A in skin keratinocytes PMID: 25164816
    10. The F464Y substitution in GPRC6A may be a cause of primary testicular failure. PMID: 23728177
    11. Extracellular Ca2+ activates the NLRP3 inflammasome via CaSR and GPRC6A. PMID: 23271661
    12. the GPRC6A receptor functions as an amino acid sensor in GLUTag cells that promotes GLP-1 secretion PMID: 23269670
    13. Our results provide further support for association of the C2orf43, FOXP4, GPRC6A and RFX6 genes with prostate cancer in Eastern Asian populations PMID: 22662242
    14. GPRC6A is a novel molecular target for regulating prostate growth and cancer progression. PMID: 21681779
    15. Observational study of gene-disease association. (HuGE Navigator) PMID: 20654748
    16. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 20676098
    17. that GPRC6A gene polymorphisms were significantly associated with human spine BMD. PMID: 19874200
    18. GPRC6A is widely expressed in brain and peripheral tissues with highest levels in kidney, skeletal muscle, testis, and leucocytes. PMID: 15194188
    19. elucidating agonist interaction with GPRC6A, a novel family C G-protein-coupled receptor. PMID: 15576628
    20. GPRC6A is a cation-, calcimimetic-, and osteocalcin-sensing receptor PMID: 16199532

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 3 family
  • 组织特异性：
    Isoform 1 is expressed at high level in brain, skeletal muscle, testis, bone, calvaria, osteoblasts and leukocytes. Expressed at intermediate level in liver, heart, kidney and spleen. Expressed at low level in lung, pancreas, placenta and ovary. Not detec
  • 数据库链接：

    HGNC: 18510

    OMIM: 613572

    KEGG: hsa:222545

    STRING: 9606.ENSP00000309493

    UniGene: Hs.266745