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  4. GIT1 Antibody

GIT1 Antibody

  • 货号：
    CSB-PA002688
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of HepG2 cells using GIT1 Polyclonal Antibody
    • Western Blot analysis of NIH-3T3 A549 cells using GIT1 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q9Y2X7
  • 基因名：
  • 别名：
    ARF GAP GIT1 antibody; ARF GTPase activating protein GIT1 antibody; ARF GTPase-activating protein GIT1 antibody; CAT 1 antibody; CAT-1 antibody; CaT1 antibody; Cool associated and tyrosine phosphorylated protein 1 antibody; Cool-associated and tyrosine-phosphorylated protein 1 antibody; G protein coupled receptor kinase interacting ArfGAP 1 antibody; G protein coupled receptor kinase interactor 1 antibody; G protein-coupled receptor kinase-interactor 1 antibody; GIT1 antibody; GIT1_HUMAN antibody; GRK interacting protein 1 antibody; GRK-interacting protein 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the C-terminal region of Human GIT1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    GTPase-activating protein for ADP ribosylation factor family members, including ARF1. Multidomain scaffold protein that interacts with numerous proteins and therefore participates in many cellular functions, including receptor internalization, focal adhesion remodeling, and signaling by both G protein-coupled receptors and tyrosine kinase receptors. Through PAK1 activation, positively regulates microtubule nucleation during interphase. Plays a role in the regulation of cytokinesis; for this function, may act in a pathway also involving ENTR1 and PTPN13. May promote cell motility both by regulating focal complex dynamics and by local activation of RAC1. May act as scaffold for MAPK1/3 signal transduction in focal adhesions. Recruits MAPK1/3/ERK1/2 to focal adhesions after EGF stimulation via a Src-dependent pathway, hence stimulating cell migration. Plays a role in brain development and function. Involved in the regulation of spine density and synaptic plasticity that is required for processes involved in learning. Plays an important role in dendritic spine morphogenesis and synapse formation. In hippocampal neurons, recruits guanine nucleotide exchange factors (GEFs), such as ARHGEF7/beta-PIX, to the synaptic membrane. These in turn locally activate RAC1, which is an essential step for spine morphogenesis and synapse formation. May contribute to the organization of presynaptic active zones through oligomerization and formation of a Piccolo/PCLO-based protein network, which includes ARHGEF7/beta-PIX and FAK1. In neurons, through its interaction with liprin-alpha family members, may be required for AMPA receptor (GRIA2/3) proper targeting to the cell membrane. In complex with GABA(A) receptors and ARHGEF7, plays a crucial role in regulating GABA(A) receptor synaptic stability, maintaining GPHN/gephyrin scaffolds and hence GABAergic inhibitory synaptic transmission, by locally coordinating RAC1 and PAK1 downstream effector activity, leading to F-actin stabilization. May also be important for RAC1 downstream signaling pathway through PAK3 and regulation of neuronal inhibitory transmission at presynaptic input. Required for successful bone regeneration during fracture healing. The function in intramembranous ossification may, at least partly, exerted by macrophages in which GIT1 is a key negative regulator of redox homeostasis, IL1B production, and glycolysis, acting through the ERK1/2/NRF2/NFE2L2 axis. May play a role in angiogenesis during fracture healing. In this process, may regulate activation of the canonical NF-kappa-B signal in bone mesenchymal stem cells by enhancing the interaction between NEMO and 'Lys-63'-ubiquitinated RIPK1/RIP1, eventually leading to enhanced production of VEGFA and others angiogenic factors. Essential for VEGF signaling through the activation of phospholipase C-gamma and ERK1/2, hence may control endothelial cell proliferation and angiogenesis.
  • 基因功能参考文献：
    1. There is significant interaction effects of the GIT1 and DRD4 gene variants on impulsivity in Attention Deficit Hyperactivity Disorder. PMID: 28948080
    2. We show that GIT1, which also contains an ANK domain, inhibits the Notch1-Dll4 signaling pathway by competing with Notch1 ANK domain for binding to RBP-J in stalk cells PMID: 27926858
    3. we discuss recent findings in key physiological systems that exemplify current understanding of the function of this important regulatory complex. Further, we draw attention to gaps in crucial information that remain to be filled to allow a better understanding of the many roles of the GIT-PIX complex in health and disease PMID: 27182061
    4. interactions between Cat-1 and its binding partner paxillin are necessary to ensure sufficient Akt activation so that cancer cells are able to grow under anchorage-independent conditions PMID: 28100775
    5. In astrocytes and neural stem cells, we identified G-protein-coupled receptor kinase-interacting protein 1 (GIT1) as a novel mTOR-binding protein, creating a unique mTOR complex lacking Raptor and Rictor PMID: 27340174
    6. miR-195 inhibits chondrocyte migration through targeted regulation of GIT1 expression. PMID: 27922692
    7. MiR-491 has a role in attenuating cancer stem cells-like properties of hepatocellular carcinoma by inhibition of GIT-1/NF-kappaB-mediated EMT PMID: 26188902
    8. Data show that G-protein-coupled receptor kinase-interacting protein 1 (GIT1) and semaphorin 4C (SEMA4C) were found to have putative microRNA miR-138 binding sites in their 3' untranslated region (3'UTRs). PMID: 26283050
    9. Data show association of G protein-coupled receptor kinase-interacting protein 1 (GIT1), p21-activated kinase interacting exchange factor (betaPIX), and p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1) with centrosomes. PMID: 27012601
    10. Results suggested that cyclic phosphorylation-dephosphorylation at Tyr- 554 of Git1 was crucial for dynamic interactions between Git1 and paxillin/Hic-5 in order to ensure coordinated cell motility. PMID: 25742295
    11. GIT1/betaPIX/Rac1/PAK pathway plays a crucial role in regulating GABA(A)R synaptic stability and hence inhibitory synaptic transmission with important implications for inhibitory plasticity and information processing in the brain. PMID: 25284783
    12. Results show that GIT1 is targeted by miR-149 to suppress breast cancer cell migration/invasion and metastasis. PMID: 24608434
    13. miRNA-491-5p and GIT1 serve as modulators and biomarkers for oral squamous cell carcinoma invasion and metastasis. PMID: 24335959
    14. GIT1-cortactin association through GIT1-Spa homology domain is required for cortactin localization to the leading edge and is essential for endothelial cell directional migration and tumor angiogenesis. PMID: 24265417
    15. GIT1 SNP rs550818 is not associated with ADHD in the Brazilian sample. PMID: 22897819
    16. novel roles for Cat-1 and its interactions with the Arf GTPases and paxillin in oncogenic transformation. PMID: 22807447
    17. Rac1 affects small GTPase Arf6 via the Arf GTPase-activating protein, GIT1, as key downstream components of the signaling pathway underlying Ras-induced non-apoptotic cell death. PMID: 20713492
    18. an intronic single-nucleotide polymorphism in GIT1, the minor allele of which causes reduced GIT1 expression, shows a strong association with ADHD susceptibility PMID: 21499268
    19. The CC domain of ERK1/2 is necessary for binding to GIT1, for ERK1/2 activation in focal adhesions, and for cell spreading and migration. PMID: 19947948
    20. MYO18A is a novel binding partner of the PAK2/betaPIX/GIT1 complex and suggest that MYO18A may play an important role in regulating epithelial cell migration via affecting multiple cell machineries. PMID: 19923322
    21. GIT1 functions in a motile, multi-molecular signaling complex that regulates membrane protrusions and cell migration. PMID: 11896197
    22. GIT1 is a novel regulator of PLCgamma function that mediates PLCgamma activation by c-Src and integrates signal transduction by GPCRs and TKRs PMID: 14523024
    23. GIT1 is a novel mediator in agonist-dependent signaling in ECs. GIT1 is involved in cell shape changes and may have a role as a negative feedback regulator that augments recovery of cell contraction. PMID: 15016733
    24. A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington disease. PMID: 15383276
    25. GIT1 has a role as a scaffold for ERK1/2 activation in focal adhesions PMID: 15923189
    26. GTI proteins regulate cytoskeletal dynamics by feedback inhibition of Rac1, they participate in receptor internalization by regulating membrane trafficking between the plasma membrane and endosomes. PMID: 16598076
    27. Mass spectrometry was to used to generate a phosphorylation map og GIT1. PMID: 16825424
    28. Results suggest that the PAK-PIX-GIT1 complex is critical for Erk-dependent myosin phosphorylation and vascular permeability. PMID: 17429073
    29. GIT1 utilizes a focal adhesion targeting-homology domain to bind paxillin. PMID: 17467235
    30. The association of PLCgamma1 with complexes containing GIT1 and beta-Pix is essential for its role in integrin-mediated cell spreading and motility. As a component of this complex, PLCgamma1 is also involved in the activation of Cdc42 and Rac1. PMID: 17562871
    31. tyrosine phosphorylation of GIT1 by Src kinases may regulate cytoskeletal reorganization downstream of alpha(IIb)beta(3) by bringing the Rac guanine nucleotide exchange factor betaPIX to the vicinity of the integrin PMID: 18211801
    32. GIT1 mediates VEGF-induced podosome formation in endothelial cells: critical role for PLCgamma. PMID: 19023093
    33. Rac3 inhibits adhesion and differentiation of neuronal cells by modifying GIT1 downstream signaling. PMID: 19494130
    34. Cloning of human GIT1 cDNA, and demonstration of GIT1 interaction with PIX and paxillin proteins. PMID: 10896954

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  • 亚细胞定位：
    Cytoplasm. Cell junction, synapse. Cell junction, synapse, presynapse. Cell junction, synapse, postsynapse. Cell junction, synapse, postsynaptic density. Cell junction, focal adhesion. Cell projection, lamellipodium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • 数据库链接：

    HGNC: 4272

    OMIM: 608434

    KEGG: hsa:28964

    STRING: 9606.ENSP00000378338

    UniGene: Hs.514051