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FUNDC1 Antibody

  • 货号：
    CSB-PA816886LA01HU
  • 规格：
    ￥3,900
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate
      All lanes: FUNDC1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 18 kDa
      Observed band size: 18 kDa
    • Western Blot
      Positive WB detected in: Mouse Brain tissue lysate, Mouse Liver tissue lysate, Rat Brain tissue lysate, Rat Liver tissue lysate
      All lanes: FUNDC1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 18 kDa
      Observed band size: 18 kDa
    • IHC image of CSB-PA816886LA01HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.
    • Immunofluorescence staining of A549 cell with CSB-PA816886LA01HU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The FUNDC1 antibody was derived from the rabbits immunized with the recombinant human FUNDC1 protein (1-46aa). This rabbit anti-human FUNDC1 polyclonal antibody was demonstrated to recognize human, mouse, and rat FUNDC1 proteins in ELISA and IHC applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity.

    The target protein FUNDC1 mainly regulates mitophagy by serving as a receptor that recruits damaged or dysfunctional mitochondria to the autophagosome for degradation. It is also involved in regulating mitochondrial morphology and dynamics, as well as various physiological and pathological processes.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) FUNDC1 Polyclonal antibody
  • Uniprot No.：
    Q8IVP5
  • 基因名：
  • 别名：
    FUNDC1; FUN14 domain-containing protein 1
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human FUN14 domain-containing protein 1 protein (96-155AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，FUNDC1 Antibody (CSB-PA816886LA01HU)，的标记方式是Non-conjugated。对于FUNDC1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA816886LB01HU FUNDC1 Antibody, HRP conjugated ELISA
    FITC CSB-PA816886LC01HU FUNDC1 Antibody, FITC conjugated
    Biotin CSB-PA816886LD01HU FUNDC1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.02% sodium azide Constituents: 50% Glycerol, PBS, pH 7.3
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IF 1:10-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.
  • 基因功能参考文献：
    1. Our data suggested that FUNDC1 can be used as a prognostic biomarker in patients with cervical cancer, and may be a new therapeutic target to improve the antitumor effects of chemoradiotherapy. PMID: 28719148
    2. phosphorylation of Tyr18 of FUNDC1 serves as a molecular switch for mitophagy. PMID: 27653272
    3. FUNDC1 integrates mitochondrial fission and mitophagy at the interface of the endoplasmic reticulum-mitochondrial contact site by working in concert with DRP1 and calnexin under hypoxic conditions in mammalian cells. PMID: 27145933
    4. FUNDC1 regulates both mitochondrial fission or fusion and mitophagy. PMID: 27050458
    5. The BCL2L1-PGAM5-FUNDC1 axis is critical for receptor-mediated mitophagy. PMID: 25126723
    6. FUNDC1 regulates ULK1 recruitment to damaged mitochondria, where FUNDC1 phosphorylation by ULK1 is crucial for mitophagy. PMID: 24671035
    7. Data show that Knockdown of endogenous FUNDC1 significantly prevented hypoxia-induced mitophagy, which could be reversed by the expression of wild-type FUNDC1. PMID: 22267086

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  • 亚细胞定位：
    Mitochondrion outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    FUN14 family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 28746

    OMIM: 300871

    KEGG: hsa:139341

    STRING: 9606.ENSP00000367284

    UniGene: Hs.7549