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Egln3 Antibody

  • 货号：
    CSB-PA14797A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Rat brain tissue, Mouse brain tissue
      All lanes: Egln3 antibody at 3.6µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 28 kDa
      Observed band size: 35 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Mus musculus (Mouse) Egln3 Polyclonal antibody
  • Uniprot No.：
    Q91UZ4
  • 基因名：
  • 别名：
    Egln3Egl nine homolog 3 antibody; EC 1.14.11.29 antibody; Hypoxia-inducible factor prolyl hydroxylase 3 antibody; HIF-PH3 antibody; HIF-prolyl hydroxylase 3 antibody; HPH-3 antibody; Prolyl hydroxylase domain-containing protein 3 antibody; PHD3 antibody; SM-20 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Mouse, Rat
  • 免疫原：
    Recombinant Mouse Egl nine homolog 3 protein (2-239AA)
  • 免疫原种属：
    Mus musculus (Mouse)
  • 标记方式：
    Non-conjugated

    本页面中的产品，Egln3 Antibody (CSB-PA14797A0Rb)，的标记方式是Non-conjugated。对于Egln3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA14797B0Rb Egln3 Antibody, HRP conjugated ELISA
    FITC CSB-PA14797C0Rb Egln3 Antibody, FITC conjugated
    Biotin CSB-PA14797D0Rb Egln3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as PKM, TELO2, ATF4 and HIF1A. Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. ELGN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4.
  • 基因功能参考文献：
    1. we identified the beta2 adrenergic receptor (ADRB2) as a downstream target for PHD3 in skeletal muscle PMID: 28939592
    2. PHD3 could protect against cardiac perivascular fibrosis and improve myocardial function in an obstructive sleep apnea mouse model by inhibiting endothelial-to-mesenchymal transition. PMID: 29051216
    3. PHD3 expression induced by cytokines is NF-kappaB dependent in mesangial cells. Endogenously produced NO further augments PHD3 expression via HIF-1 alpha. PMID: 28054119
    4. Opposing regulation and roles for PHD3 in lung dendritic cells and alveolar macrophages PMID: 28716863
    5. Our observations disclose a novel role of PHD3 in the development of Tregs. PMID: 27331863
    6. PHD3 is an active participant in atherogenesis PMID: 26995088
    7. Cardiomyocyte-specific transgenic expression of PHD3 impairs the myocardial response to ischemia. PMID: 26044310
    8. PHD3 protects intestinal epithelial barrier function and reveal a hydroxylase-independent function of PHD3 in stabilizing occludin PMID: 26124271
    9. depletion of PHD3 leads to increased stabilization of HIF-1alpha and inhibition of DNA damage response, both of which may contribute to the cardioprotective effect seen with depletion of PHD3. PMID: 25633836
    10. PHD3 loss sustains cell proliferation through the control of EGFR. PMID: 25420773
    11. Using conditional PHD3-knockout mice, it was shown that PHD3 affects the production of Angptl2 and additionally influences the response toward this apoptosis-modulating factor. PMID: 24626957
    12. ablation of the PHD3 gene resulted in increased angiogenesis and cardiac function after infarction thereby offering a potential target for management of ischemic myocardial disease PMID: 23978105
    13. isoform-specific inhibition of Phd3 could be exploited to treat type 2 diabetes without the toxicity that could occur with chronic inhibition of multiple Phd isoforms. PMID: 24037093
    14. Egln3 suppresses glioma progression PMID: 22905089
    15. mice lacking PHD3 were resistant to the effects of ionizing radiation and had decreased thymic apoptosis, a biomarker of genomic integrity PMID: 22797300
    16. It is concluded that impairment of PHD3 enzyme function aggravates the clinical course of abdominal sepsis PMID: 22786772
    17. PHD3 has a role in regulating neutrophil survival in hypoxia PMID: 21317538
    18. EGLN3 has a role in the regulation of NF-kappaB and suggests that it is involved in mediating myogenic differentiation, which is HIF-independent PMID: 20089853
    19. PHD3 appears to be a tumor suppressor in colorectal cancer cells that inhibits IKKbeta/NF-kappaB signaling, independent of its hydroxylase activity. PMID: 19786027
    20. Egln1/2/3 play dual roles in chondrocyted growth, acting as oxygen sensors and mediators of late stage events in the cell cycle. PMID: 17044072
    21. the role of PHD3 in sympathoadrenal development extends beyond simple control of cell survival and organ mass, with functional PHD3 being required for proper anatomical and physiological integrity of the system PMID: 18332118
    22. Phd3 loss exacerbates the HIF activation, hepatic steatosis, dilated cardiomyopathy, and premature mortality. PMID: 19720742

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 组织特异性：
    Highly expressed in cardiac and smooth muscle. Also high expression in brain, skeletal muscle and kidney. Low levels in lung.
  • 数据库链接：

    KEGG: mmu:112407

    STRING: 10090.ENSMUSP00000041874

    UniGene: Mm.133037