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ESPL1 Antibody

  • 货号：
    CSB-PA004066
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of A431 cells using Separase Polyclonal Antibody
    • Western Blot analysis of HuvEc cells using Separase Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q14674
  • 基因名：
  • 别名：
    Caspase like protein ESPL1 antibody; Caspase-like protein ESPL1 antibody; ESP 1 antibody; ESP1 antibody; ESPL 1 antibody; Espl1 antibody; ESPL1_HUMAN antibody; Extra spindle poles like 1 antibody; Extra spindle poles like 1 protein antibody; Extra spindle poles-like 1 protein antibody; FLJ46492 antibody; KIAA0165 antibody; Separase antibody; Separin antibody; Similar to fission yeast cut1and gene antibody; SSE antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from Human Separase around the non-phosphorylation site of S801.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:40000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
  • 基因功能参考文献：
    1. Separase activity measurement may therefore be useful as a novel additional molecular marker for disease monitoring PMID: 29370237
    2. Studies identified and characterized the role of separase in mitosis, meiosis, non-canonical roles, its regulation, as a regulator of centriole disengagement, non proteolytic roles, diverse substrates, structural insights, and association of separase with cancer. [review] PMID: 27966791
    3. High ESP expression is associated with breast cancer. PMID: 28859055
    4. Proximity mapping of human separase has been presented. PMID: 27495871
    5. The assay was used to quantify Separase proteolytic activity in leukemic cell lines and peripheral blood samples from leukemia patients. PMID: 26267133
    6. Recruitment and activation of separase at centrosomes are two distinct steps that do not require microtubules. PMID: 25299182
    7. Separase protein levels decrease and Separase proteolytic activity increases exclusively in b3a2 p210BCR-ABL-positive cell lines under Imatinib treatment. PMID: 26087013
    8. Separase is an oncogene whose overexpression induces tumorigenesis, and indicate that Separase overexpression and aberrant nuclear localization are common in many tumor types and may predict outcome in some human malignancies. PMID: 24792645
    9. High ESPL1 mRNA expression was associated with luminal B breast cancers. PMID: 25086634
    10. Data indicate that separase is subject to native-state cis/trans isomerization by peptidyl-prolyl-isomerase Pin1. PMID: 25921067
    11. By consecutively acting as a protease and a cdk1 inhibitor, separase coordinates two key processes to achieve simultaneous and abrupt separation of sister chromatids. PMID: 22814604
    12. Kendrin is a novel and crucial substrate for separase (ESPL1) at the centrosome, protecting the engaged centrioles from premature disengagement and thereby blocking reduplication until the cell passes through mitosis. PMID: 22542101
    13. Plk1-mediated phosphorylation of Cdc6 promotes the interaction of Cdc6 and Cdk1, leading to the attenuation of Cdk1 activity, release of separase, and subsequent anaphase progression. PMID: 21041660
    14. Anaphase specific auto-cleavage of separase. PMID: 12297314
    15. Processing, localization, and requirement of separase for normal anaphase progression. PMID: 12672959
    16. Separase function is not restricted to anaphase initiation; its role in promoting loss of sister chromatid cohesion might be preferentially at arms but not centromeres. PMID: 16177575
    17. Protein phosphatase 2A and separase form a complex regulated by separase autocleavage PMID: 17604273
    18. Phosphorylation promotes complex formation indirectly, possibly by inducing a conformational change in full-length separase. PMID: 17974570
    19. The complete removal of cohesin from chromosome arms depends on separase. PMID: 18003702
    20. Separase has targets involved in regulation of G(2) to M progression after DNA damage in lung cancer cells. PMID: 18616699
    21. These results collectively suggest that Separase is an oncogene, whose overexpression alone in mammary epithelial cells is sufficient to induce aneuploidy and tumorigenesis in a p53 mutant background. PMID: 18728194
    22. Study reports that cohesin cleavage by human separase requires DNA in a sequence-nonspecific manner. PMID: 19345191
    23. Separase might be an oncogene, whose overexpression induces tumorigenesis. Separase overexpression and aberrant nuclear localization are common in many tumor types and may predict outcome in some human cancers. PMID: 19351757
    24. Plk1 and separase act at different times during M phase to license centrosome duplication, reminiscent of their roles in removing cohesin from chromosomes PMID: 19758559

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    HGNC: 16856

    OMIM: 604143

    KEGG: hsa:9700

    STRING: 9606.ENSP00000257934

    UniGene: Hs.153479